Characterization And In Vitro Study Of Protoporphyrin Ix (Haem) Aptamer In Reversing Drug-Resistant Malaria
The global health challenge of malaria, compounded by drug resistance, necessitates innovative approaches for effective treatment. Aptamer technology is a promising tool towards combatting drug-resistant malaria especially chloroquine (cq) resistance. Preliminary study on protoporphyrin ix (haem) dn...
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| Format: | Thesis |
| Language: | English |
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2024
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| Online Access: | http://eprints.usm.my/62688/ http://eprints.usm.my/62688/1/24%20Pages%20from%20ALIYU%20ABDULWAHAB.pdf |
| _version_ | 1848885061226594304 |
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| author | Abdulwahab, Aliyu |
| author_facet | Abdulwahab, Aliyu |
| author_sort | Abdulwahab, Aliyu |
| building | USM Institutional Repository |
| collection | Online Access |
| description | The global health challenge of malaria, compounded by drug resistance, necessitates innovative approaches for effective treatment. Aptamer technology is a promising tool towards combatting drug-resistant malaria especially chloroquine (cq) resistance. Preliminary study on protoporphyrin ix (haem) dna-aptamers (oka_24 and oka_26 ) demonstrated an anti-malarial property but lack the ability to internalise into the parasite-infected red blood cell (rbc). This research investigates the potential of cholesterol-tri ethylene glycol (col-teg) modified haem aptamers in addressing drug-resistant malaria. The research employs a multifaceted approach, including in silico techniques for predicting aptamer structures and molecular docking studies to assess binding behaviour. Additionally, reverse-phase high-performance liquid-chromatography (rp-hplc) was utilized to evaluate serum stability, while uv-absorption spectral titration and square wave voltammetry (swv) provided insights into the specificity and affinity of modified aptamers for haem. Cellular internalization assays, conducted using fluorescence-microscopy and flow cytometry, determine the efficiency of col-teg-modified aptamers in entering red blood cells. The study also examines the antimalarial activity of modified aptamers against cq-sensitive (3d7-strain) and cq-resistant (w2-strain) plasmodium falciparum. Docking analysis reveals that the transformation of oka_26 to col-teg-oka_26 does not alter binding behaviour, |
| first_indexed | 2025-11-15T19:16:36Z |
| format | Thesis |
| id | usm-62688 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T19:16:36Z |
| publishDate | 2024 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-626882025-07-28T02:58:39Z http://eprints.usm.my/62688/ Characterization And In Vitro Study Of Protoporphyrin Ix (Haem) Aptamer In Reversing Drug-Resistant Malaria Abdulwahab, Aliyu R5-920 Medicine (General) The global health challenge of malaria, compounded by drug resistance, necessitates innovative approaches for effective treatment. Aptamer technology is a promising tool towards combatting drug-resistant malaria especially chloroquine (cq) resistance. Preliminary study on protoporphyrin ix (haem) dna-aptamers (oka_24 and oka_26 ) demonstrated an anti-malarial property but lack the ability to internalise into the parasite-infected red blood cell (rbc). This research investigates the potential of cholesterol-tri ethylene glycol (col-teg) modified haem aptamers in addressing drug-resistant malaria. The research employs a multifaceted approach, including in silico techniques for predicting aptamer structures and molecular docking studies to assess binding behaviour. Additionally, reverse-phase high-performance liquid-chromatography (rp-hplc) was utilized to evaluate serum stability, while uv-absorption spectral titration and square wave voltammetry (swv) provided insights into the specificity and affinity of modified aptamers for haem. Cellular internalization assays, conducted using fluorescence-microscopy and flow cytometry, determine the efficiency of col-teg-modified aptamers in entering red blood cells. The study also examines the antimalarial activity of modified aptamers against cq-sensitive (3d7-strain) and cq-resistant (w2-strain) plasmodium falciparum. Docking analysis reveals that the transformation of oka_26 to col-teg-oka_26 does not alter binding behaviour, 2024-07 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/62688/1/24%20Pages%20from%20ALIYU%20ABDULWAHAB.pdf Abdulwahab, Aliyu (2024) Characterization And In Vitro Study Of Protoporphyrin Ix (Haem) Aptamer In Reversing Drug-Resistant Malaria. PhD thesis, Perpustakaan Hamzah Sendut. |
| spellingShingle | R5-920 Medicine (General) Abdulwahab, Aliyu Characterization And In Vitro Study Of Protoporphyrin Ix (Haem) Aptamer In Reversing Drug-Resistant Malaria |
| title | Characterization And In Vitro Study Of Protoporphyrin Ix (Haem) Aptamer In Reversing Drug-Resistant Malaria |
| title_full | Characterization And In Vitro Study Of Protoporphyrin Ix (Haem) Aptamer In Reversing Drug-Resistant Malaria |
| title_fullStr | Characterization And In Vitro Study Of Protoporphyrin Ix (Haem) Aptamer In Reversing Drug-Resistant Malaria |
| title_full_unstemmed | Characterization And In Vitro Study Of Protoporphyrin Ix (Haem) Aptamer In Reversing Drug-Resistant Malaria |
| title_short | Characterization And In Vitro Study Of Protoporphyrin Ix (Haem) Aptamer In Reversing Drug-Resistant Malaria |
| title_sort | characterization and in vitro study of protoporphyrin ix (haem) aptamer in reversing drug-resistant malaria |
| topic | R5-920 Medicine (General) |
| url | http://eprints.usm.my/62688/ http://eprints.usm.my/62688/1/24%20Pages%20from%20ALIYU%20ABDULWAHAB.pdf |