Antimicrobial activity of aqueous Quercus infectoria gall extract against pathogenic Leptospira
Leptospirosis is a bacterial zoonotic infectious disease that occurs worldwide caused by Leptospira and transmitted to human through direct or indirect contact with the urine of infected animal. Quercus infectoria gall extract is known to have a broad spectrum antimicrobial activity in-vitro. Thi...
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| Format: | Monograph |
| Language: | English |
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Universiti Sains Malaysia
2016
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| Online Access: | http://eprints.usm.my/62590/ http://eprints.usm.my/62590/1/HUSNA%20BINTI%20MUSTAFA%20-%20e.pdf |
| Summary: | Leptospirosis is a bacterial zoonotic infectious disease that occurs worldwide caused by
Leptospira and transmitted to human through direct or indirect contact with the urine of
infected animal. Quercus infectoria gall extract is known to have a broad spectrum antimicrobial
activity in-vitro. This study was conducted to determine the antimicrobial
activity of Q. infectoria gall extract against pathogenic Leptospira and to evaluate the
morphological changes of extract-treated cells using scanning electron microscope
(SEM). Two-fold serial microdilution broth assay was used to determine the minimum
inhibitory concentration (MIC) of aqueous Q. infectoria gall extract against L.
interrogans serovar Javanica dan L. interrogans serovar Icterohaemorhagiae at
concentration ranging from 4.00 mg/mL to 0.0078 mg/mL. Minimum bactericidal
concentration (MBC) was determined by sub-culturing the broth from microtitre plate
well showing no apparent growth or turbidity into the freshly prepared EMJH media
and observed under dark field microscope after 3 weeks incubation for growth detection. The cell morphology of extract-treated and untreated L. interrogans serovar Icterohaemorhagiae were analyzed using SEM. The results of broth microdilution assay
demonstrated that aqueous Q. infectoria gall extract possessed antimicrobial activity
against both L. interrogans serovar with MIC values of 0.125 mg/mL. The MBC values
for L. interrogans serovar Javanica dan L. interrogans serovar Icterohaemorhagiae were
0.125 mg/mL and 0.25 mg/mL respectively. The SEM micrograph showed changes of shape and size of the L. interrogans serovar Icterohaemorrhagiae extract-treated cells
(at 8x MIC) as compared to the untreated cells. In conclusion, aqueous Q. infectoria gall
extract has the potential to be explored for its efficacy and use in the treatment of
leptospirosis. |
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