Enhancement Of Cellulase-Poor Xylanase Production By Native Fungi Via Solid State Fermentation Process
High cost of cellulases remains the most significant barrier to the economical production of bio-ethanol from lignocellulosic biomass (LB). The present study aims at developing a local cellulolytic fungal strain with enhanced cellulolytic ability through random mutagenesis coupled with the feasib...
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| Format: | Thesis |
| Language: | English |
| Published: |
2016
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| Subjects: | |
| Online Access: | http://eprints.usm.my/62547/ http://eprints.usm.my/62547/1/24%20Pages%20from%2000001808069.pdf |
| _version_ | 1848885017796673536 |
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| author | Tai, Wan Yi |
| author_facet | Tai, Wan Yi |
| author_sort | Tai, Wan Yi |
| building | USM Institutional Repository |
| collection | Online Access |
| description | High cost of cellulases remains the most significant barrier to the economical
production of bio-ethanol from lignocellulosic biomass (LB). The present study aims at
developing a local cellulolytic fungal strain with enhanced cellulolytic ability through
random mutagenesis coupled with the feasibility of solid-state fermentation (SSF) by
utilizing oil palm frond (OPF) as the substrate. Out of 95 wild isolates isolated from
agricultural sites and tested both qualitatively and quantitatively, a native filamentous
fungal strain designated DWA8 was found to be the top enzymatic secretor. During
qualitative screening, 38 isolates were found to produce distinctive halo but only 23
were chosen to proceed with quantitative screening. For quantitative enzyme analysis,
enzyme supernatant was extracted from the SSF process which was conducted using
IxlO6 spore/mL inoculated onto 5 g of ground OPF, incubated at room temperature for 7
days. DWA8 was found to be the best candidate for further studies, as it produced the
highest amount of fpase and xylanase with a considerably high amount of cmcase, which
Aspergillus niger (Genebank accession no. KP299287) based on colony morphology and
the best cultivation period where the cmcase, fpase and xylanase activities of A. niger
DWA8 were 1.13 U/g, 2.55 U/g and 2.38 U/g respectively. |
| first_indexed | 2025-11-15T19:15:55Z |
| format | Thesis |
| id | usm-62547 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T19:15:55Z |
| publishDate | 2016 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-625472025-06-23T04:44:29Z http://eprints.usm.my/62547/ Enhancement Of Cellulase-Poor Xylanase Production By Native Fungi Via Solid State Fermentation Process Tai, Wan Yi T1-995 Technology(General) High cost of cellulases remains the most significant barrier to the economical production of bio-ethanol from lignocellulosic biomass (LB). The present study aims at developing a local cellulolytic fungal strain with enhanced cellulolytic ability through random mutagenesis coupled with the feasibility of solid-state fermentation (SSF) by utilizing oil palm frond (OPF) as the substrate. Out of 95 wild isolates isolated from agricultural sites and tested both qualitatively and quantitatively, a native filamentous fungal strain designated DWA8 was found to be the top enzymatic secretor. During qualitative screening, 38 isolates were found to produce distinctive halo but only 23 were chosen to proceed with quantitative screening. For quantitative enzyme analysis, enzyme supernatant was extracted from the SSF process which was conducted using IxlO6 spore/mL inoculated onto 5 g of ground OPF, incubated at room temperature for 7 days. DWA8 was found to be the best candidate for further studies, as it produced the highest amount of fpase and xylanase with a considerably high amount of cmcase, which Aspergillus niger (Genebank accession no. KP299287) based on colony morphology and the best cultivation period where the cmcase, fpase and xylanase activities of A. niger DWA8 were 1.13 U/g, 2.55 U/g and 2.38 U/g respectively. 2016-09 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/62547/1/24%20Pages%20from%2000001808069.pdf Tai, Wan Yi (2016) Enhancement Of Cellulase-Poor Xylanase Production By Native Fungi Via Solid State Fermentation Process. Masters thesis, Perpustakaan Hamzah Sendut. |
| spellingShingle | T1-995 Technology(General) Tai, Wan Yi Enhancement Of Cellulase-Poor Xylanase Production By Native Fungi Via Solid State Fermentation Process |
| title | Enhancement Of Cellulase-Poor
Xylanase Production By Native Fungi
Via Solid State Fermentation Process |
| title_full | Enhancement Of Cellulase-Poor
Xylanase Production By Native Fungi
Via Solid State Fermentation Process |
| title_fullStr | Enhancement Of Cellulase-Poor
Xylanase Production By Native Fungi
Via Solid State Fermentation Process |
| title_full_unstemmed | Enhancement Of Cellulase-Poor
Xylanase Production By Native Fungi
Via Solid State Fermentation Process |
| title_short | Enhancement Of Cellulase-Poor
Xylanase Production By Native Fungi
Via Solid State Fermentation Process |
| title_sort | enhancement of cellulase-poor
xylanase production by native fungi
via solid state fermentation process |
| topic | T1-995 Technology(General) |
| url | http://eprints.usm.my/62547/ http://eprints.usm.my/62547/1/24%20Pages%20from%2000001808069.pdf |