Screening Of Α-Glucosidase Inhibitors From Black Soybean Extract And Study On Their Inhibitory Mechanisms
Various studies have documented the beneficial effects of black soybean extract against diabetes. However, the specific active compounds responsible for these effects remain elusive. This study aimed at identifying the α-glucosidase inhibitors present in black soybeans using affinity ultrafiltration...
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| Format: | Thesis |
| Language: | English |
| Published: |
2024
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| Online Access: | http://eprints.usm.my/62343/ http://eprints.usm.my/62343/1/ZHENG%20YU%20-%20TESIS%20cut.pdf |
| _version_ | 1848884956764307456 |
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| author | Zheng, Yu |
| author_facet | Zheng, Yu |
| author_sort | Zheng, Yu |
| building | USM Institutional Repository |
| collection | Online Access |
| description | Various studies have documented the beneficial effects of black soybean extract against diabetes. However, the specific active compounds responsible for these effects remain elusive. This study aimed at identifying the α-glucosidase inhibitors present in black soybeans using affinity ultrafiltration. Seven isoflavones with α-glucosidase inhibitory activity were identified, among which daidzein and genistein demonstrated remarkably superior inhibitory activity compared to that of acarbose (IC50 = 632.5 ± 70.0 μmol/mL), exhibiting IC50 values of 15.7 ± 0.3 and 3.2 ± 1.2 μmol/mL, respectively. An analysis of the structure-activity relationship revealed a negative correlation between the degree of glycosylation of isoflavone and α-glucosidase inhibitory activity. Enzyme kinetic studies revealed that both daidzein and genistein acted as uncompetitive inhibitors of α-glucosidase. Additionally, these compounds effectively suppressed the intrinsic fluorescence of α-glucosidase through the static quenching mechanism. The interaction between daidzein or genistein and α-glucosidase involves spontaneous formation of a complex, which is driven by hydrophobic interactions and hydrogen bonds. These findings provide valuable insights into the molecular mechanisms underlying the α-glucosidase inhibitory activity of black soybean isoflavones. |
| first_indexed | 2025-11-15T19:14:57Z |
| format | Thesis |
| id | usm-62343 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T19:14:57Z |
| publishDate | 2024 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-623432025-05-27T08:03:43Z http://eprints.usm.my/62343/ Screening Of Α-Glucosidase Inhibitors From Black Soybean Extract And Study On Their Inhibitory Mechanisms Zheng, Yu T11.95-12.5 Industrial directories Various studies have documented the beneficial effects of black soybean extract against diabetes. However, the specific active compounds responsible for these effects remain elusive. This study aimed at identifying the α-glucosidase inhibitors present in black soybeans using affinity ultrafiltration. Seven isoflavones with α-glucosidase inhibitory activity were identified, among which daidzein and genistein demonstrated remarkably superior inhibitory activity compared to that of acarbose (IC50 = 632.5 ± 70.0 μmol/mL), exhibiting IC50 values of 15.7 ± 0.3 and 3.2 ± 1.2 μmol/mL, respectively. An analysis of the structure-activity relationship revealed a negative correlation between the degree of glycosylation of isoflavone and α-glucosidase inhibitory activity. Enzyme kinetic studies revealed that both daidzein and genistein acted as uncompetitive inhibitors of α-glucosidase. Additionally, these compounds effectively suppressed the intrinsic fluorescence of α-glucosidase through the static quenching mechanism. The interaction between daidzein or genistein and α-glucosidase involves spontaneous formation of a complex, which is driven by hydrophobic interactions and hydrogen bonds. These findings provide valuable insights into the molecular mechanisms underlying the α-glucosidase inhibitory activity of black soybean isoflavones. 2024-07 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/62343/1/ZHENG%20YU%20-%20TESIS%20cut.pdf Zheng, Yu (2024) Screening Of Α-Glucosidase Inhibitors From Black Soybean Extract And Study On Their Inhibitory Mechanisms. Masters thesis, Universiti Sains Malaysia. |
| spellingShingle | T11.95-12.5 Industrial directories Zheng, Yu Screening Of Α-Glucosidase Inhibitors From Black Soybean Extract And Study On Their Inhibitory Mechanisms |
| title | Screening Of Α-Glucosidase Inhibitors From Black Soybean Extract And Study On Their Inhibitory Mechanisms |
| title_full | Screening Of Α-Glucosidase Inhibitors From Black Soybean Extract And Study On Their Inhibitory Mechanisms |
| title_fullStr | Screening Of Α-Glucosidase Inhibitors From Black Soybean Extract And Study On Their Inhibitory Mechanisms |
| title_full_unstemmed | Screening Of Α-Glucosidase Inhibitors From Black Soybean Extract And Study On Their Inhibitory Mechanisms |
| title_short | Screening Of Α-Glucosidase Inhibitors From Black Soybean Extract And Study On Their Inhibitory Mechanisms |
| title_sort | screening of α-glucosidase inhibitors from black soybean extract and study on their inhibitory mechanisms |
| topic | T11.95-12.5 Industrial directories |
| url | http://eprints.usm.my/62343/ http://eprints.usm.my/62343/1/ZHENG%20YU%20-%20TESIS%20cut.pdf |