In Vitro And In Vivo Effects Of Bisphenol A On Cell Growth, Lipid Accumulation And Inflammation In Liver Cell Line, Rat Adipose And Colorectal Tissues
Bisphenol A (BPA) is an exogenous endocrine disruptor that mimics hormones closely associated with health complications, such as cancer progression. BPA is also linked to an increase in the prevalence of obesity and obesity-related diseases due to its obesogenic action. The present study aimed to ev...
| Main Author: | |
|---|---|
| Format: | Thesis |
| Language: | English |
| Published: |
2024
|
| Subjects: | |
| Online Access: | http://eprints.usm.my/62273/ http://eprints.usm.my/62273/1/24%20Pages%20from%20ISMAEL%20LAYLA%20QASIM%20ISMAEL.pdf |
| _version_ | 1848884937381380096 |
|---|---|
| author | Ismael, Ismael Layla Qasim |
| author_facet | Ismael, Ismael Layla Qasim |
| author_sort | Ismael, Ismael Layla Qasim |
| building | USM Institutional Repository |
| collection | Online Access |
| description | Bisphenol A (BPA) is an exogenous endocrine disruptor that mimics hormones closely associated with health complications, such as cancer progression. BPA is also linked to an increase in the prevalence of obesity and obesity-related diseases due to its obesogenic action. The present study aimed to evaluate the effects of BPA on human liver cells and animal tissues. The BPA-treated THLE-2 cell viability was first evaluated using an MTT assay. Cytoplasmic lipid accumulation was then examined in the BPA-treated THLE-2 cells using Oil Red O staining. The expression of various adipogenesis genes in lipid metabolism, including clusterin, PPARs, CYPs, UCP1, and BRS3 in BPA-treated THLE‑2 cells, was investigated using real-time PCR. Subsequently, flow cytometry measured the DNA synthesis in BPA-treated THLE-2 cells was performed using BrdU/PI staining. The pro-inflammatory proteins IL6 and CCL2 in the conditioned media and cytoplasmic proteins of the BPA-treated THLE-2 cells were examined by ELISA. After that, various adipogenesis genes’ expression in lipid metabolism, including clusterin, PPARs, CYPs, UCP1 and BRS3, in adipose and colorectal tissues of the rats fed a diet (normal or high fat) supplemented with and without BPA was investigated using real-time PCR. |
| first_indexed | 2025-11-15T19:14:38Z |
| format | Thesis |
| id | usm-62273 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T19:14:38Z |
| publishDate | 2024 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-622732025-05-22T02:17:11Z http://eprints.usm.my/62273/ In Vitro And In Vivo Effects Of Bisphenol A On Cell Growth, Lipid Accumulation And Inflammation In Liver Cell Line, Rat Adipose And Colorectal Tissues Ismael, Ismael Layla Qasim R5-920 Medicine (General) Bisphenol A (BPA) is an exogenous endocrine disruptor that mimics hormones closely associated with health complications, such as cancer progression. BPA is also linked to an increase in the prevalence of obesity and obesity-related diseases due to its obesogenic action. The present study aimed to evaluate the effects of BPA on human liver cells and animal tissues. The BPA-treated THLE-2 cell viability was first evaluated using an MTT assay. Cytoplasmic lipid accumulation was then examined in the BPA-treated THLE-2 cells using Oil Red O staining. The expression of various adipogenesis genes in lipid metabolism, including clusterin, PPARs, CYPs, UCP1, and BRS3 in BPA-treated THLE‑2 cells, was investigated using real-time PCR. Subsequently, flow cytometry measured the DNA synthesis in BPA-treated THLE-2 cells was performed using BrdU/PI staining. The pro-inflammatory proteins IL6 and CCL2 in the conditioned media and cytoplasmic proteins of the BPA-treated THLE-2 cells were examined by ELISA. After that, various adipogenesis genes’ expression in lipid metabolism, including clusterin, PPARs, CYPs, UCP1 and BRS3, in adipose and colorectal tissues of the rats fed a diet (normal or high fat) supplemented with and without BPA was investigated using real-time PCR. 2024-03 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/62273/1/24%20Pages%20from%20ISMAEL%20LAYLA%20QASIM%20ISMAEL.pdf Ismael, Ismael Layla Qasim (2024) In Vitro And In Vivo Effects Of Bisphenol A On Cell Growth, Lipid Accumulation And Inflammation In Liver Cell Line, Rat Adipose And Colorectal Tissues. PhD thesis, Perpustakaan Hamzah Sendut. |
| spellingShingle | R5-920 Medicine (General) Ismael, Ismael Layla Qasim In Vitro And In Vivo Effects Of Bisphenol A On Cell Growth, Lipid Accumulation And Inflammation In Liver Cell Line, Rat Adipose And Colorectal Tissues |
| title | In Vitro And In Vivo Effects Of Bisphenol A On Cell Growth, Lipid Accumulation And Inflammation In Liver Cell Line, Rat Adipose And Colorectal Tissues |
| title_full | In Vitro And In Vivo Effects Of Bisphenol A On Cell Growth, Lipid Accumulation And Inflammation In Liver Cell Line, Rat Adipose And Colorectal Tissues |
| title_fullStr | In Vitro And In Vivo Effects Of Bisphenol A On Cell Growth, Lipid Accumulation And Inflammation In Liver Cell Line, Rat Adipose And Colorectal Tissues |
| title_full_unstemmed | In Vitro And In Vivo Effects Of Bisphenol A On Cell Growth, Lipid Accumulation And Inflammation In Liver Cell Line, Rat Adipose And Colorectal Tissues |
| title_short | In Vitro And In Vivo Effects Of Bisphenol A On Cell Growth, Lipid Accumulation And Inflammation In Liver Cell Line, Rat Adipose And Colorectal Tissues |
| title_sort | in vitro and in vivo effects of bisphenol a on cell growth, lipid accumulation and inflammation in liver cell line, rat adipose and colorectal tissues |
| topic | R5-920 Medicine (General) |
| url | http://eprints.usm.my/62273/ http://eprints.usm.my/62273/1/24%20Pages%20from%20ISMAEL%20LAYLA%20QASIM%20ISMAEL.pdf |