Effects Of Droplet-Vitrification Cryopreservation Method On Ludisia Discolor For Long-Term Germplasm Conservation
Ludisia belongs to a group of slow-growth terrestrial orchids cultivated for their attractive ornamental leaves, commonly referred to as ‘Jewel Orchids’. They are steadily dwindling in their natural population through habitat loss and indiscriminate collection of wild orchids. Therefore, cryopreserv...
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| Format: | Thesis |
| Language: | English |
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2024
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| Online Access: | http://eprints.usm.my/62257/ http://eprints.usm.my/62257/1/24%20Pages%20from%20HAZIRAH%20BINTI%20BURKHAN.pdf |
| _version_ | 1848884932827414528 |
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| author | Burkhan, Hazirah |
| author_facet | Burkhan, Hazirah |
| author_sort | Burkhan, Hazirah |
| building | USM Institutional Repository |
| collection | Online Access |
| description | Ludisia belongs to a group of slow-growth terrestrial orchids cultivated for their attractive ornamental leaves, commonly referred to as ‘Jewel Orchids’. They are steadily dwindling in their natural population through habitat loss and indiscriminate collection of wild orchids. Therefore, cryopreservation as a long-term storage approach offers a complementary conservation strategy that safeguards against accidental loss. Although some cryopreservation methods have been developed for several genera of the Orchidaceae family, the cryopreservation method for Ludisia species has not been documented before. Therefore, this study reported the first attempt of long-term storage of L. discolor, using a droplet-vitrification method. The present study sought to examine the effects of cryopreserving L. discolor orchid supported with qualitative analyses using microscopy, biochemical, and molecular. Critical parameters such as preculture sucrose concentrations and durations, plant vitrification solution 2 (PVS2) durations and temperatures, growth recovery medium, and different light-emitting diode (LED) spectra were assessed. The survivability of axillary bud was determined using 2,3,5-triphenyltetrazolium chloride (TTC) spectrophotometric analysis and visual inspection. |
| first_indexed | 2025-11-15T19:14:34Z |
| format | Thesis |
| id | usm-62257 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T19:14:34Z |
| publishDate | 2024 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-622572025-05-21T02:00:38Z http://eprints.usm.my/62257/ Effects Of Droplet-Vitrification Cryopreservation Method On Ludisia Discolor For Long-Term Germplasm Conservation Burkhan, Hazirah QH1-278.5 Natural history (General) Ludisia belongs to a group of slow-growth terrestrial orchids cultivated for their attractive ornamental leaves, commonly referred to as ‘Jewel Orchids’. They are steadily dwindling in their natural population through habitat loss and indiscriminate collection of wild orchids. Therefore, cryopreservation as a long-term storage approach offers a complementary conservation strategy that safeguards against accidental loss. Although some cryopreservation methods have been developed for several genera of the Orchidaceae family, the cryopreservation method for Ludisia species has not been documented before. Therefore, this study reported the first attempt of long-term storage of L. discolor, using a droplet-vitrification method. The present study sought to examine the effects of cryopreserving L. discolor orchid supported with qualitative analyses using microscopy, biochemical, and molecular. Critical parameters such as preculture sucrose concentrations and durations, plant vitrification solution 2 (PVS2) durations and temperatures, growth recovery medium, and different light-emitting diode (LED) spectra were assessed. The survivability of axillary bud was determined using 2,3,5-triphenyltetrazolium chloride (TTC) spectrophotometric analysis and visual inspection. 2024-04 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/62257/1/24%20Pages%20from%20HAZIRAH%20BINTI%20BURKHAN.pdf Burkhan, Hazirah (2024) Effects Of Droplet-Vitrification Cryopreservation Method On Ludisia Discolor For Long-Term Germplasm Conservation. PhD thesis, Perpustakaan Hamzah Sendut. |
| spellingShingle | QH1-278.5 Natural history (General) Burkhan, Hazirah Effects Of Droplet-Vitrification Cryopreservation Method On Ludisia Discolor For Long-Term Germplasm Conservation |
| title | Effects Of Droplet-Vitrification Cryopreservation Method On Ludisia Discolor For Long-Term Germplasm Conservation |
| title_full | Effects Of Droplet-Vitrification Cryopreservation Method On Ludisia Discolor For Long-Term Germplasm Conservation |
| title_fullStr | Effects Of Droplet-Vitrification Cryopreservation Method On Ludisia Discolor For Long-Term Germplasm Conservation |
| title_full_unstemmed | Effects Of Droplet-Vitrification Cryopreservation Method On Ludisia Discolor For Long-Term Germplasm Conservation |
| title_short | Effects Of Droplet-Vitrification Cryopreservation Method On Ludisia Discolor For Long-Term Germplasm Conservation |
| title_sort | effects of droplet-vitrification cryopreservation method on ludisia discolor for long-term germplasm conservation |
| topic | QH1-278.5 Natural history (General) |
| url | http://eprints.usm.my/62257/ http://eprints.usm.my/62257/1/24%20Pages%20from%20HAZIRAH%20BINTI%20BURKHAN.pdf |