Anti-bacterial activity of Oroxylum indicum methanolic leaves extract against gram negative bacteria
lndicum is used as a traditional remedy of many ailments and its leave extract has been reported to have antimicrobial properties. The purpose of this study was to evaluate the in vitro anti-bacterial activity of the methanolic leaves extract of O. indicum against selected bacteria species. Methano...
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| Format: | Monograph |
| Language: | English |
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Universiti Sains Malaysia
2017
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| Online Access: | http://eprints.usm.my/62142/ http://eprints.usm.my/62142/1/NUR%20FATIN%20JULIA%20NABILA%20BINTI%20ZULKIPLI%20-%20e.pdf |
| Summary: | lndicum is used as a traditional remedy of many ailments and its leave extract has been reported to have antimicrobial properties. The purpose of this study was to evaluate the in vitro anti-bacterial activity of the methanolic leaves extract of O. indicum
against selected bacteria species. Methanolic leaves extract of O. indicum was tested for
anti-bacterial activity against E. coli, S.flexneri, P. mirabilis, P. vulgaris, P. aeruginosa, K.
pneumoniae, and S. Typhi. Anti-bacterial activities were evaluated by well-diffusion
method and the Minimum Inhibitory Concentration (MIC) was determined using the twofold
serial broth microdilution technique of concentration ranging from 150 mg/mL to 1.17
mg/mL. After 24 hours of incubation, the Minimum Bactericidal Concentration (MBC) was
determined by sub-culturing the wells which showed no turbidity onto Nutrient agar (NA).
The presence of potential secondary metabolites in the crude extract was screened by
phytochemical qualitative test. The methanolic leaves extract of O. indicum showed good
anti-bacterial activity and P. vulgaris being the most susceptible among the tested bacteria
species. The methanolic leaves extract of O. indicum also bactericidal to P. vulgaris and S.
flexneri (MBC/MIC ratio of 4.00). Flavonoid, tannin, triterpenoid, and steroid were the
major secondary metabolites of the crude extract. The study suggested that O. indicum
methanolic leaves extract has promising anti-bacterial activity. |
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