Determination of total microorganisms count and vibrio species profile in freshwater fish
Fish consist of freshwater fish and marine fish are known as one of the main protein sources in human diet. Freshwater fish have a great taste, rich with nutritive values and many other health benefits. However, freshwater fish appealed to be a potential vector of Vibrio microorganisms such as V....
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| Format: | Monograph |
| Language: | English |
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Universiti Sains Malaysia
2017
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| Online Access: | http://eprints.usm.my/62138/ http://eprints.usm.my/62138/1/NOR%20AQILAH%20BINTI%20MOHAMED%20AZMI%20-%20e.pdf |
| _version_ | 1848884900240818176 |
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| author | Azmi, Nor Aqilah Mohamed |
| author_facet | Azmi, Nor Aqilah Mohamed |
| author_sort | Azmi, Nor Aqilah Mohamed |
| building | USM Institutional Repository |
| collection | Online Access |
| description | Fish consist of freshwater fish and marine fish are known as one of the main protein
sources in human diet. Freshwater fish have a great taste, rich with nutritive values and
many other health benefits. However, freshwater fish appealed to be a potential vector of
Vibrio microorganisms such as V. cholerae, V. parahaemolyticus and V. vulnificus. Yet,
very little information about the microorganisms risk assessments especially Vibrio in
freshwater fish as there is no standard of permissible for Vibrio microorganisms in
Malaysia. In this project, a study was conducted in order to assess the microorganisms
risk and presence of Vibrio in freshwater fish. Different species of freshwater fish (n=10)
were obtained from wet markets within Kota Bharu, Kelantan. The total microorganisms
count was determined using spread plate method and Vibrio profile used Thiosulfate
Citrate Bile Salts Sucrose (TCBS) agar, Tryptone Salt (T1N1) agar for purity and salt
tolerance test for Vibrio spp. confirmation test. The results had shown that ‘Tilapia Hitam’ (Oreochromis mossambicus') had the highest total microorganisms count at 3.34 106 cfu/g as compared to the other freshwater fish samples. The limit for total microorganisms
count in fishery products of Malaysia is at 106 per gram, and 80% of the freshwater fish
samples in this study were within the permissible limit set by Ministry of Health Malaysia.
For the Vibrio microorganisms profile, 20% of freshwater fish samples detected with both
V. cholerae and V. parahaemolyticus, while 80% from the freshwater fish samples had
only V. cholerae. In conclusion, it was suggested that Vibrio can be found in freshwater
fish. Thus, proper handling of freshwater fish must be practiced in order to decrease the
incidence of foodbome illness due to these microorganisms. Further research should be done to enhance the food safety of freshwater fish consumption, hence able to set the
standard of permissible for Vibrio microorganisms in Malaysia. |
| first_indexed | 2025-11-15T19:14:03Z |
| format | Monograph |
| id | usm-62138 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T19:14:03Z |
| publishDate | 2017 |
| publisher | Universiti Sains Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-621382025-06-29T07:47:24Z http://eprints.usm.my/62138/ Determination of total microorganisms count and vibrio species profile in freshwater fish Azmi, Nor Aqilah Mohamed R Medicine (General) RA Public aspects of medicine Fish consist of freshwater fish and marine fish are known as one of the main protein sources in human diet. Freshwater fish have a great taste, rich with nutritive values and many other health benefits. However, freshwater fish appealed to be a potential vector of Vibrio microorganisms such as V. cholerae, V. parahaemolyticus and V. vulnificus. Yet, very little information about the microorganisms risk assessments especially Vibrio in freshwater fish as there is no standard of permissible for Vibrio microorganisms in Malaysia. In this project, a study was conducted in order to assess the microorganisms risk and presence of Vibrio in freshwater fish. Different species of freshwater fish (n=10) were obtained from wet markets within Kota Bharu, Kelantan. The total microorganisms count was determined using spread plate method and Vibrio profile used Thiosulfate Citrate Bile Salts Sucrose (TCBS) agar, Tryptone Salt (T1N1) agar for purity and salt tolerance test for Vibrio spp. confirmation test. The results had shown that ‘Tilapia Hitam’ (Oreochromis mossambicus') had the highest total microorganisms count at 3.34 106 cfu/g as compared to the other freshwater fish samples. The limit for total microorganisms count in fishery products of Malaysia is at 106 per gram, and 80% of the freshwater fish samples in this study were within the permissible limit set by Ministry of Health Malaysia. For the Vibrio microorganisms profile, 20% of freshwater fish samples detected with both V. cholerae and V. parahaemolyticus, while 80% from the freshwater fish samples had only V. cholerae. In conclusion, it was suggested that Vibrio can be found in freshwater fish. Thus, proper handling of freshwater fish must be practiced in order to decrease the incidence of foodbome illness due to these microorganisms. Further research should be done to enhance the food safety of freshwater fish consumption, hence able to set the standard of permissible for Vibrio microorganisms in Malaysia. Universiti Sains Malaysia 2017 Monograph NonPeerReviewed application/pdf en http://eprints.usm.my/62138/1/NOR%20AQILAH%20BINTI%20MOHAMED%20AZMI%20-%20e.pdf Azmi, Nor Aqilah Mohamed (2017) Determination of total microorganisms count and vibrio species profile in freshwater fish. Project Report. Universiti Sains Malaysia. (Submitted) |
| spellingShingle | R Medicine (General) RA Public aspects of medicine Azmi, Nor Aqilah Mohamed Determination of total microorganisms count and vibrio species profile in freshwater fish |
| title | Determination of total
microorganisms count and vibrio
species profile in freshwater fish |
| title_full | Determination of total
microorganisms count and vibrio
species profile in freshwater fish |
| title_fullStr | Determination of total
microorganisms count and vibrio
species profile in freshwater fish |
| title_full_unstemmed | Determination of total
microorganisms count and vibrio
species profile in freshwater fish |
| title_short | Determination of total
microorganisms count and vibrio
species profile in freshwater fish |
| title_sort | determination of total
microorganisms count and vibrio
species profile in freshwater fish |
| topic | R Medicine (General) RA Public aspects of medicine |
| url | http://eprints.usm.my/62138/ http://eprints.usm.my/62138/1/NOR%20AQILAH%20BINTI%20MOHAMED%20AZMI%20-%20e.pdf |