Development Of Tcr-like Single Domain Antibody By Incorporating Immunoglobulin Isotypes For Latent Tuberculosis Diagnostics And Therapeutics Application Against Heat Shock Protein 16 Kda Peptide Presented By Hla-a2
Heat shock protein 16 kDa (HSP 16 kDa) is essential for the survival of latent Mycobacterium tuberculosis. Therefore, a peptide-MHC presentation of HSP 16 kDa could be a potential diagnostic and therapeutic target for latent tuberculosis (LTB). This study aimed to generate different immunoglobuli...
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| Format: | Thesis |
| Language: | English |
| Published: |
2024
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| Online Access: | http://eprints.usm.my/62136/ http://eprints.usm.my/62136/1/LIU%20HUAQIANG%20-%20TESIS24.pdf |
| _version_ | 1848884899303391232 |
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| author | Liu, Hua Qiang |
| author_facet | Liu, Hua Qiang |
| author_sort | Liu, Hua Qiang |
| building | USM Institutional Repository |
| collection | Online Access |
| description | Heat shock protein 16 kDa (HSP 16 kDa) is essential for the survival of latent
Mycobacterium tuberculosis. Therefore, a peptide-MHC presentation of HSP 16
kDa could be a potential diagnostic and therapeutic target for latent tuberculosis
(LTB). This study aimed to generate different immunoglobulin isotype pairings on a
TCR-like single-domain antibody (sDAb) and subsequently investigate its
diagnostic and therapeutic potential in LTB, utilizing a model cell presenting the
target peptide. A previously generated TCR-like sDAb that can bind to HSP 16 kDa
was first fused to a human IgG1, IgG2, IgG4, IgA, IgD, IgE, and IgM Fc-receptor
via a linker. The fusion product, sDAb-Fc, was expressed in HEK293-F cells and
subsequently purified. Its diagnostic potential was investigated via cell-based
ELISA utilizing MCF-7 cells peptide-pulsed with HSP 16 kDa peptides.
Antibody-dependent cell-mediated cytotoxicity (ADCC) of MCF-7 cells was also
conducted to investigate its therapeutic potential. Finally, TCR-like sDAb-IgG1,
IgG2, and IgG4 were successfully produced transiently in HEK293 F cells and
purified using protein A chromatography. |
| first_indexed | 2025-11-15T19:14:02Z |
| format | Thesis |
| id | usm-62136 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T19:14:02Z |
| publishDate | 2024 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-621362025-04-29T03:23:02Z http://eprints.usm.my/62136/ Development Of Tcr-like Single Domain Antibody By Incorporating Immunoglobulin Isotypes For Latent Tuberculosis Diagnostics And Therapeutics Application Against Heat Shock Protein 16 Kda Peptide Presented By Hla-a2 Liu, Hua Qiang R5-920 Medicine (General) Heat shock protein 16 kDa (HSP 16 kDa) is essential for the survival of latent Mycobacterium tuberculosis. Therefore, a peptide-MHC presentation of HSP 16 kDa could be a potential diagnostic and therapeutic target for latent tuberculosis (LTB). This study aimed to generate different immunoglobulin isotype pairings on a TCR-like single-domain antibody (sDAb) and subsequently investigate its diagnostic and therapeutic potential in LTB, utilizing a model cell presenting the target peptide. A previously generated TCR-like sDAb that can bind to HSP 16 kDa was first fused to a human IgG1, IgG2, IgG4, IgA, IgD, IgE, and IgM Fc-receptor via a linker. The fusion product, sDAb-Fc, was expressed in HEK293-F cells and subsequently purified. Its diagnostic potential was investigated via cell-based ELISA utilizing MCF-7 cells peptide-pulsed with HSP 16 kDa peptides. Antibody-dependent cell-mediated cytotoxicity (ADCC) of MCF-7 cells was also conducted to investigate its therapeutic potential. Finally, TCR-like sDAb-IgG1, IgG2, and IgG4 were successfully produced transiently in HEK293 F cells and purified using protein A chromatography. 2024-08 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/62136/1/LIU%20HUAQIANG%20-%20TESIS24.pdf Liu, Hua Qiang (2024) Development Of Tcr-like Single Domain Antibody By Incorporating Immunoglobulin Isotypes For Latent Tuberculosis Diagnostics And Therapeutics Application Against Heat Shock Protein 16 Kda Peptide Presented By Hla-a2. PhD thesis, Universiti Sains Malaysia. |
| spellingShingle | R5-920 Medicine (General) Liu, Hua Qiang Development Of Tcr-like Single Domain Antibody By Incorporating Immunoglobulin Isotypes For Latent Tuberculosis Diagnostics And Therapeutics Application Against Heat Shock Protein 16 Kda Peptide Presented By Hla-a2 |
| title | Development Of Tcr-like Single
Domain Antibody By Incorporating
Immunoglobulin Isotypes For Latent
Tuberculosis Diagnostics And
Therapeutics Application Against
Heat Shock Protein 16 Kda Peptide
Presented By Hla-a2 |
| title_full | Development Of Tcr-like Single
Domain Antibody By Incorporating
Immunoglobulin Isotypes For Latent
Tuberculosis Diagnostics And
Therapeutics Application Against
Heat Shock Protein 16 Kda Peptide
Presented By Hla-a2 |
| title_fullStr | Development Of Tcr-like Single
Domain Antibody By Incorporating
Immunoglobulin Isotypes For Latent
Tuberculosis Diagnostics And
Therapeutics Application Against
Heat Shock Protein 16 Kda Peptide
Presented By Hla-a2 |
| title_full_unstemmed | Development Of Tcr-like Single
Domain Antibody By Incorporating
Immunoglobulin Isotypes For Latent
Tuberculosis Diagnostics And
Therapeutics Application Against
Heat Shock Protein 16 Kda Peptide
Presented By Hla-a2 |
| title_short | Development Of Tcr-like Single
Domain Antibody By Incorporating
Immunoglobulin Isotypes For Latent
Tuberculosis Diagnostics And
Therapeutics Application Against
Heat Shock Protein 16 Kda Peptide
Presented By Hla-a2 |
| title_sort | development of tcr-like single
domain antibody by incorporating
immunoglobulin isotypes for latent
tuberculosis diagnostics and
therapeutics application against
heat shock protein 16 kda peptide
presented by hla-a2 |
| topic | R5-920 Medicine (General) |
| url | http://eprints.usm.my/62136/ http://eprints.usm.my/62136/1/LIU%20HUAQIANG%20-%20TESIS24.pdf |