Subcloning of influenza a ha-na synthetic gene in bacterial system
Avian influenza A H5N1 virus continues to cause outbreaks around the globe since its first emergence in 1997 in Hong Kong. The highly pathogenic virus is capable of undergoing antigenic drifts and antigenic shifts as mechanisms enabling it to evade any immunity induced against it raising the alar...
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| Format: | Monograph |
| Language: | English |
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Universiti Sains Malaysia
2017
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| Online Access: | http://eprints.usm.my/62037/ http://eprints.usm.my/62037/1/MARWA%20NORUDDIN%20MAHAMMAD%20HASSAN%20-%20e.pdf |
| _version_ | 1848884872512274432 |
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| author | Hassan, Marwa Noruddin Mahammad |
| author_facet | Hassan, Marwa Noruddin Mahammad |
| author_sort | Hassan, Marwa Noruddin Mahammad |
| building | USM Institutional Repository |
| collection | Online Access |
| description | Avian influenza A H5N1 virus continues to cause outbreaks around the globe since its
first emergence in 1997 in Hong Kong. The highly pathogenic virus is capable of
undergoing antigenic drifts and antigenic shifts as mechanisms enabling it to evade any
immunity induced against it raising the alarm of potential pandemics and outbreaks.
These mechanisms were found to influence two pathogenic determinants of Influenza A
H5N1, which are the surface glycoprotein heamagglutinin HA and surface protein
neuraminidase NA. These molecules are a principal target when it comes to vaccines
production to eradicate the potentially deadly virus. In this project, highly
immunogenic/hydrophilic regions of HA and NA were constructed and characterized
through cloning. The synthetic gene HA-NA was successfully cloned where the
assembling with the authentic synthetic gene sequence displayed 100 % similarity. The
successfully cloned gene can be utilized for the production of highly immunogenic
vaccine which is capable of producing sustainable immunity across generations of the
virus, thus lightening the strain of producing annual vaccine in order to combat the
newly arising variant. Additionally, it can be used as a tool to understand the
immunogenic features of the hybrid gene which can assist in further understanding of
the body mechanism in tackling the viral infections. |
| first_indexed | 2025-11-15T19:13:36Z |
| format | Monograph |
| id | usm-62037 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T19:13:36Z |
| publishDate | 2017 |
| publisher | Universiti Sains Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-620372025-03-24T07:53:03Z http://eprints.usm.my/62037/ Subcloning of influenza a ha-na synthetic gene in bacterial system Hassan, Marwa Noruddin Mahammad R Medicine (General) RC109-216 Infectious and parasitic diseases Avian influenza A H5N1 virus continues to cause outbreaks around the globe since its first emergence in 1997 in Hong Kong. The highly pathogenic virus is capable of undergoing antigenic drifts and antigenic shifts as mechanisms enabling it to evade any immunity induced against it raising the alarm of potential pandemics and outbreaks. These mechanisms were found to influence two pathogenic determinants of Influenza A H5N1, which are the surface glycoprotein heamagglutinin HA and surface protein neuraminidase NA. These molecules are a principal target when it comes to vaccines production to eradicate the potentially deadly virus. In this project, highly immunogenic/hydrophilic regions of HA and NA were constructed and characterized through cloning. The synthetic gene HA-NA was successfully cloned where the assembling with the authentic synthetic gene sequence displayed 100 % similarity. The successfully cloned gene can be utilized for the production of highly immunogenic vaccine which is capable of producing sustainable immunity across generations of the virus, thus lightening the strain of producing annual vaccine in order to combat the newly arising variant. Additionally, it can be used as a tool to understand the immunogenic features of the hybrid gene which can assist in further understanding of the body mechanism in tackling the viral infections. Universiti Sains Malaysia 2017 Monograph NonPeerReviewed application/pdf en http://eprints.usm.my/62037/1/MARWA%20NORUDDIN%20MAHAMMAD%20HASSAN%20-%20e.pdf Hassan, Marwa Noruddin Mahammad (2017) Subcloning of influenza a ha-na synthetic gene in bacterial system. Project Report. Universiti Sains Malaysia. (Submitted) |
| spellingShingle | R Medicine (General) RC109-216 Infectious and parasitic diseases Hassan, Marwa Noruddin Mahammad Subcloning of influenza a ha-na synthetic gene in bacterial system |
| title | Subcloning of influenza a ha-na synthetic gene in
bacterial system |
| title_full | Subcloning of influenza a ha-na synthetic gene in
bacterial system |
| title_fullStr | Subcloning of influenza a ha-na synthetic gene in
bacterial system |
| title_full_unstemmed | Subcloning of influenza a ha-na synthetic gene in
bacterial system |
| title_short | Subcloning of influenza a ha-na synthetic gene in
bacterial system |
| title_sort | subcloning of influenza a ha-na synthetic gene in
bacterial system |
| topic | R Medicine (General) RC109-216 Infectious and parasitic diseases |
| url | http://eprints.usm.my/62037/ http://eprints.usm.my/62037/1/MARWA%20NORUDDIN%20MAHAMMAD%20HASSAN%20-%20e.pdf |