Anti-candida activity of methanolic oroxylum indicum leaves extract against candida species
Oroxylum indicum has been used traditionally to treat common ailments and its leave extract has been reported to possess antimicrobial properties. The aim of this study was to evaluate the in-vitro anti-Candida activity of Oroxylum indicum leaves extract against selected Candida species. Methanol...
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| Format: | Monograph |
| Language: | English |
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Universiti Sains Malaysia
2024
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| Online Access: | http://eprints.usm.my/61997/ http://eprints.usm.my/61997/1/AMALINA%20BINTI%20AB%20RAHMAN%20-%20e.pdf |
| Summary: | Oroxylum indicum has been used traditionally to treat common ailments and its leave
extract has been reported to possess antimicrobial properties. The aim of this study was
to evaluate the in-vitro anti-Candida activity of Oroxylum indicum leaves extract
against selected Candida species. Methanolic leaves extract of Oroxylum indicum was
tested for anti-Candida activity against C. albicans, C. tropicalis, C. parapsilopsis, C.
krusei, and C. glabrata. Anti-Candida activities were evaluated by well-diffusion
method and the minimum inhibitory concentrations (MIC) were determined using the
two-fold serial broth microdilution technique of concentration ranging from 500.0
mg/mL to 3.9 mg/mL. After 24 hours of incubation, the minimum fungicidal
concentration (MFC) was determined by sub-culturing the wells which showed no turbidity onto Sabouraud dextrose agar (SDA). The presence of potential phytochemicals group in the crude extract was screened by phytochemical qualitative
tests. The methanolic extract showed moderate (50% of inhibition zone) anti-Candida
activity against C. albicans and C. krusei. C. albicans was the most susceptible among
the tested Candida species and showed a low MIC value (78 mg/mL). The extract was
also fungicidal to C. albicans (MFC/MIC ratio of 4.0). Flavonoid, tannin, triterpenoid
and steroid were the major phytochemicals of the crude extract. This study suggested
that methanolic Oroxylum indicum leaves extract has weak anti-Candida activity. |
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