Metabolomics Analysis Of Blood And Urine To Identify Alcohol-Dependence Biomarkers
Alcohol misuse is a ravaging public health and social problem. Alcoholdependence (AD) is a phase of alcohol misuse in which the drinker consumes excessive amount of alcohol and have a continuous urge to consume alcohol. The current methods of alcohol dependence diagnosis are questionnaires and so...
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| Format: | Thesis |
| Language: | English |
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2015
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| Online Access: | http://eprints.usm.my/61164/ http://eprints.usm.my/61164/1/24%20Pages%20from%2000001792372.pdf |
| _version_ | 1848884636211478528 |
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| author | Mostafa, Hamza Mohamed Amin |
| author_facet | Mostafa, Hamza Mohamed Amin |
| author_sort | Mostafa, Hamza Mohamed Amin |
| building | USM Institutional Repository |
| collection | Online Access |
| description | Alcohol misuse is a ravaging public health and social problem. Alcoholdependence
(AD) is a phase of alcohol misuse in which the drinker consumes
excessive amount of alcohol and have a continuous urge to consume alcohol. The
current methods of alcohol dependence diagnosis are questionnaires and some
biomarkers. However, both methods lack specificity and sensitivity. Metabolomics is
a novel scientific field which may provide a novel method for the diagnosis of
alcohol-dependence by using a sensitive and specific technique such as nuclear
magnetic resonance (NMR). Our aim was to identify the metabolic fingerprint
(metabotype) that is able to discriminate between AD, non-AD alcohol drinkers and
controls using metabolomics approach. The reproducibility of the outcomes was also
investigated. Blood and urine samples were collected from 30 alcohol-dependent
(mean age: 45.7), 54 social drinkers (mean age: 39.5) and 60 controls (mean age:
37.1). Plasma was separated by centrifugation, then both plasma and urine samples
were mixed with phosphate buffer which was prepared by dissolving di-sodium
hydrogen phosphate (Na2HP04), sodium dihydrogen phosphate (NaH2P04), sodium
3-(trimethylsilyl)-propionate-2,2,3,3- |
| first_indexed | 2025-11-15T19:09:51Z |
| format | Thesis |
| id | usm-61164 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T19:09:51Z |
| publishDate | 2015 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-611642024-09-19T04:05:05Z http://eprints.usm.my/61164/ Metabolomics Analysis Of Blood And Urine To Identify Alcohol-Dependence Biomarkers Mostafa, Hamza Mohamed Amin RS1-441 Pharmacy and materia medica Alcohol misuse is a ravaging public health and social problem. Alcoholdependence (AD) is a phase of alcohol misuse in which the drinker consumes excessive amount of alcohol and have a continuous urge to consume alcohol. The current methods of alcohol dependence diagnosis are questionnaires and some biomarkers. However, both methods lack specificity and sensitivity. Metabolomics is a novel scientific field which may provide a novel method for the diagnosis of alcohol-dependence by using a sensitive and specific technique such as nuclear magnetic resonance (NMR). Our aim was to identify the metabolic fingerprint (metabotype) that is able to discriminate between AD, non-AD alcohol drinkers and controls using metabolomics approach. The reproducibility of the outcomes was also investigated. Blood and urine samples were collected from 30 alcohol-dependent (mean age: 45.7), 54 social drinkers (mean age: 39.5) and 60 controls (mean age: 37.1). Plasma was separated by centrifugation, then both plasma and urine samples were mixed with phosphate buffer which was prepared by dissolving di-sodium hydrogen phosphate (Na2HP04), sodium dihydrogen phosphate (NaH2P04), sodium 3-(trimethylsilyl)-propionate-2,2,3,3- 2015-07 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/61164/1/24%20Pages%20from%2000001792372.pdf Mostafa, Hamza Mohamed Amin (2015) Metabolomics Analysis Of Blood And Urine To Identify Alcohol-Dependence Biomarkers. Masters thesis, Perpustakaan Hamzah Sendut. |
| spellingShingle | RS1-441 Pharmacy and materia medica Mostafa, Hamza Mohamed Amin Metabolomics Analysis Of Blood And Urine To Identify Alcohol-Dependence Biomarkers |
| title | Metabolomics Analysis Of Blood And Urine To Identify Alcohol-Dependence Biomarkers |
| title_full | Metabolomics Analysis Of Blood And Urine To Identify Alcohol-Dependence Biomarkers |
| title_fullStr | Metabolomics Analysis Of Blood And Urine To Identify Alcohol-Dependence Biomarkers |
| title_full_unstemmed | Metabolomics Analysis Of Blood And Urine To Identify Alcohol-Dependence Biomarkers |
| title_short | Metabolomics Analysis Of Blood And Urine To Identify Alcohol-Dependence Biomarkers |
| title_sort | metabolomics analysis of blood and urine to identify alcohol-dependence biomarkers |
| topic | RS1-441 Pharmacy and materia medica |
| url | http://eprints.usm.my/61164/ http://eprints.usm.my/61164/1/24%20Pages%20from%2000001792372.pdf |