Unveiling safety profile of Pereskia bleo leaf extract: A comprehensive study on female toxicity and teratogenicity in Sprague Dawley rats

Pereskia bleo (P. bleo), renowned for its traditional medicinal use in various countries, is valued for its therapeutic potential. While medicinal plants hold immense promise, concerns regarding potential adverse effects on normal cells and teratogenicity remain. This study evaluated the influence o...

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Main Authors: Khalaf, Taif Kareem, Nazri, Nor Amalia, Abdulrazak, Mohammed Hussain, Bakar, Ainul Bahiyah Abu, Ezumi, Mohd Fuad Wan, Ismail, Norzila
Format: Article
Language:English
Published: Journal of Chemical Health Risks 2024
Subjects:
Online Access:http://eprints.usm.my/60833/
http://eprints.usm.my/60833/1/Published-Dr._Norzila_Ismail_student-Manuscript_30-05-2024%5B3%5D.pdf
_version_ 1848884550380290048
author Khalaf, Taif Kareem
Nazri, Nor Amalia
Abdulrazak, Mohammed Hussain
Bakar, Ainul Bahiyah Abu
Ezumi, Mohd Fuad Wan
Ismail, Norzila
author_facet Khalaf, Taif Kareem
Nazri, Nor Amalia
Abdulrazak, Mohammed Hussain
Bakar, Ainul Bahiyah Abu
Ezumi, Mohd Fuad Wan
Ismail, Norzila
author_sort Khalaf, Taif Kareem
building USM Institutional Repository
collection Online Access
description Pereskia bleo (P. bleo), renowned for its traditional medicinal use in various countries, is valued for its therapeutic potential. While medicinal plants hold immense promise, concerns regarding potential adverse effects on normal cells and teratogenicity remain. This study evaluated the influence of methanol extract of Pereskia bleo (MEPB) leaves on female toxicity and teratogenicity in Sprague Dawley rats. Female rats (n=40) were divided into four groups receiving MEPB doses (0, 250, 500, and 1000 mg/kg/day) during pre-mating, mating, and gestation periods. Gas Chromatography-Mass Spectrometry (GC-MS) analysis exhibited that MEPB leaves contain phenolic compounds, terpenoids, phytosterols, and fatty acids. Remarkably, all the animals exhibited regular oestrous cycles, maintaining body weight, and showed no signs of toxicity or abnormal behavior. Visceral organ weights and histological analysis revealed no significant alterations, attesting to the extract's safety. Pregnancy outcomes, including corpora lutea, implantation sites, percentages of pre- and post- implantation death, gravid uterine weight, number of live and dead fetuses, fetal body weight, and fetal sex ratio, remained unaffected. Furthermore, meticulous fetal examinations confirmed that MEPB did not impact foetal parameters or induce deformities. Importantly, daily MEPB consumption (250-1000 mg/kg) from pre-pregnancy to pregnancy did not compromise maternal or fetal well-being. These results underscore the lack of embryotoxic or teratogenic effects of MEPB, highlighting its potential for safe therapeutic applications.
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spelling usm-608332024-09-11T04:12:24Z http://eprints.usm.my/60833/ Unveiling safety profile of Pereskia bleo leaf extract: A comprehensive study on female toxicity and teratogenicity in Sprague Dawley rats Khalaf, Taif Kareem Nazri, Nor Amalia Abdulrazak, Mohammed Hussain Bakar, Ainul Bahiyah Abu Ezumi, Mohd Fuad Wan Ismail, Norzila RA Public aspects of medicine RA440-440.87 Study and teaching. Research Pereskia bleo (P. bleo), renowned for its traditional medicinal use in various countries, is valued for its therapeutic potential. While medicinal plants hold immense promise, concerns regarding potential adverse effects on normal cells and teratogenicity remain. This study evaluated the influence of methanol extract of Pereskia bleo (MEPB) leaves on female toxicity and teratogenicity in Sprague Dawley rats. Female rats (n=40) were divided into four groups receiving MEPB doses (0, 250, 500, and 1000 mg/kg/day) during pre-mating, mating, and gestation periods. Gas Chromatography-Mass Spectrometry (GC-MS) analysis exhibited that MEPB leaves contain phenolic compounds, terpenoids, phytosterols, and fatty acids. Remarkably, all the animals exhibited regular oestrous cycles, maintaining body weight, and showed no signs of toxicity or abnormal behavior. Visceral organ weights and histological analysis revealed no significant alterations, attesting to the extract's safety. Pregnancy outcomes, including corpora lutea, implantation sites, percentages of pre- and post- implantation death, gravid uterine weight, number of live and dead fetuses, fetal body weight, and fetal sex ratio, remained unaffected. Furthermore, meticulous fetal examinations confirmed that MEPB did not impact foetal parameters or induce deformities. Importantly, daily MEPB consumption (250-1000 mg/kg) from pre-pregnancy to pregnancy did not compromise maternal or fetal well-being. These results underscore the lack of embryotoxic or teratogenic effects of MEPB, highlighting its potential for safe therapeutic applications. Journal of Chemical Health Risks 2024-05-28 Article PeerReviewed application/pdf en http://eprints.usm.my/60833/1/Published-Dr._Norzila_Ismail_student-Manuscript_30-05-2024%5B3%5D.pdf Khalaf, Taif Kareem and Nazri, Nor Amalia and Abdulrazak, Mohammed Hussain and Bakar, Ainul Bahiyah Abu and Ezumi, Mohd Fuad Wan and Ismail, Norzila (2024) Unveiling safety profile of Pereskia bleo leaf extract: A comprehensive study on female toxicity and teratogenicity in Sprague Dawley rats. Journal of Chemical Health Risks, 14 (3). pp. 740-755. ISSN 2251-6727
spellingShingle RA Public aspects of medicine
RA440-440.87 Study and teaching. Research
Khalaf, Taif Kareem
Nazri, Nor Amalia
Abdulrazak, Mohammed Hussain
Bakar, Ainul Bahiyah Abu
Ezumi, Mohd Fuad Wan
Ismail, Norzila
Unveiling safety profile of Pereskia bleo leaf extract: A comprehensive study on female toxicity and teratogenicity in Sprague Dawley rats
title Unveiling safety profile of Pereskia bleo leaf extract: A comprehensive study on female toxicity and teratogenicity in Sprague Dawley rats
title_full Unveiling safety profile of Pereskia bleo leaf extract: A comprehensive study on female toxicity and teratogenicity in Sprague Dawley rats
title_fullStr Unveiling safety profile of Pereskia bleo leaf extract: A comprehensive study on female toxicity and teratogenicity in Sprague Dawley rats
title_full_unstemmed Unveiling safety profile of Pereskia bleo leaf extract: A comprehensive study on female toxicity and teratogenicity in Sprague Dawley rats
title_short Unveiling safety profile of Pereskia bleo leaf extract: A comprehensive study on female toxicity and teratogenicity in Sprague Dawley rats
title_sort unveiling safety profile of pereskia bleo leaf extract: a comprehensive study on female toxicity and teratogenicity in sprague dawley rats
topic RA Public aspects of medicine
RA440-440.87 Study and teaching. Research
url http://eprints.usm.my/60833/
http://eprints.usm.my/60833/1/Published-Dr._Norzila_Ismail_student-Manuscript_30-05-2024%5B3%5D.pdf