Expression of toll-like receptor (TLR) 2 on macrophage infected with rBCG clone expressing MSP-1C of Plasmodium falciparum and its effect on IL-12 and IL-lp production
Toll like receptor (TLR) is a pattern recognition receptor (PRRs) that involve in innate immune response against invading pathogen during phagocytosis. TLR controls the transcription of inflammatory response by the infected cells. Among the TLRs identified, TLR2 is one of the receptor that involv...
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| Format: | Monograph |
| Language: | English |
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Universiti Sains Malaysia
2015
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| Online Access: | http://eprints.usm.my/60415/ http://eprints.usm.my/60415/1/NURSYAZANA%20AQILAH%20BINTI%20ALI%20-%20e.pdf |
| _version_ | 1848884439891836928 |
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| author | Ali, Nursyazana Aqilah |
| author_facet | Ali, Nursyazana Aqilah |
| author_sort | Ali, Nursyazana Aqilah |
| building | USM Institutional Repository |
| collection | Online Access |
| description | Toll like receptor (TLR) is a pattern recognition receptor (PRRs) that involve in innate
immune response against invading pathogen during phagocytosis. TLR controls the
transcription of inflammatory response by the infected cells. Among the TLRs identified,
TLR2 is one of the receptor that involved in the recognition of mycobacterium and malaria parasites by macrophages. This study was conducted to determine the expression of the
TLR2 protein in a mouse macrophage cell line infected with BCG and recombinant BCG
(rBCG) expressing the C-terminus of merozoite surface protein (MSP-1C) of Plasmodium
falciparum by Western blot. The role of the TLR in the production of pro-inflammatory
cytokine production such as IL-12 and IL-ip was also evaluated in the supernatant of infected cells in the presence or absence of TLR2 inhibitor and LPS+IFN-y stimulation using ELISA. The result demonstrated that both BCG- and rBCG-infected macrophages
express significant TLR2 protein. However, the expression of TLR2 by the rBCG-infected
macrophages was significantly higher than those expressed by BCG-infected macrophages.
For the cytokine production, the presence of TLR2 inhibitor does not affect the production
of IL-12, but capable to reduce the production of IL-ip by the macrophage infected with
both BCG and rBCG. In conclusion, our results indicate that the macrophage expresses
TLR2 receptor during BCG and rBCG infection. However, this TLR2 receptor only
important for the production of IL-ip cytokine but not the production of IL-12 cytokine by
the macrophages. |
| first_indexed | 2025-11-15T19:06:44Z |
| format | Monograph |
| id | usm-60415 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T19:06:44Z |
| publishDate | 2015 |
| publisher | Universiti Sains Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-604152024-05-06T08:00:49Z http://eprints.usm.my/60415/ Expression of toll-like receptor (TLR) 2 on macrophage infected with rBCG clone expressing MSP-1C of Plasmodium falciparum and its effect on IL-12 and IL-lp production Ali, Nursyazana Aqilah R Medicine (General) RA440-440.87 Study and teaching. Research RC Internal medicine Toll like receptor (TLR) is a pattern recognition receptor (PRRs) that involve in innate immune response against invading pathogen during phagocytosis. TLR controls the transcription of inflammatory response by the infected cells. Among the TLRs identified, TLR2 is one of the receptor that involved in the recognition of mycobacterium and malaria parasites by macrophages. This study was conducted to determine the expression of the TLR2 protein in a mouse macrophage cell line infected with BCG and recombinant BCG (rBCG) expressing the C-terminus of merozoite surface protein (MSP-1C) of Plasmodium falciparum by Western blot. The role of the TLR in the production of pro-inflammatory cytokine production such as IL-12 and IL-ip was also evaluated in the supernatant of infected cells in the presence or absence of TLR2 inhibitor and LPS+IFN-y stimulation using ELISA. The result demonstrated that both BCG- and rBCG-infected macrophages express significant TLR2 protein. However, the expression of TLR2 by the rBCG-infected macrophages was significantly higher than those expressed by BCG-infected macrophages. For the cytokine production, the presence of TLR2 inhibitor does not affect the production of IL-12, but capable to reduce the production of IL-ip by the macrophage infected with both BCG and rBCG. In conclusion, our results indicate that the macrophage expresses TLR2 receptor during BCG and rBCG infection. However, this TLR2 receptor only important for the production of IL-ip cytokine but not the production of IL-12 cytokine by the macrophages. Universiti Sains Malaysia 2015 Monograph NonPeerReviewed application/pdf en http://eprints.usm.my/60415/1/NURSYAZANA%20AQILAH%20BINTI%20ALI%20-%20e.pdf Ali, Nursyazana Aqilah (2015) Expression of toll-like receptor (TLR) 2 on macrophage infected with rBCG clone expressing MSP-1C of Plasmodium falciparum and its effect on IL-12 and IL-lp production. Project Report. Universiti Sains Malaysia. (Submitted) |
| spellingShingle | R Medicine (General) RA440-440.87 Study and teaching. Research RC Internal medicine Ali, Nursyazana Aqilah Expression of toll-like receptor (TLR) 2 on macrophage infected with rBCG clone expressing MSP-1C of Plasmodium falciparum and its effect on IL-12 and IL-lp production |
| title | Expression of toll-like receptor (TLR) 2 on macrophage
infected with rBCG clone expressing MSP-1C of Plasmodium falciparum
and its effect on IL-12 and IL-lp production |
| title_full | Expression of toll-like receptor (TLR) 2 on macrophage
infected with rBCG clone expressing MSP-1C of Plasmodium falciparum
and its effect on IL-12 and IL-lp production |
| title_fullStr | Expression of toll-like receptor (TLR) 2 on macrophage
infected with rBCG clone expressing MSP-1C of Plasmodium falciparum
and its effect on IL-12 and IL-lp production |
| title_full_unstemmed | Expression of toll-like receptor (TLR) 2 on macrophage
infected with rBCG clone expressing MSP-1C of Plasmodium falciparum
and its effect on IL-12 and IL-lp production |
| title_short | Expression of toll-like receptor (TLR) 2 on macrophage
infected with rBCG clone expressing MSP-1C of Plasmodium falciparum
and its effect on IL-12 and IL-lp production |
| title_sort | expression of toll-like receptor (tlr) 2 on macrophage
infected with rbcg clone expressing msp-1c of plasmodium falciparum
and its effect on il-12 and il-lp production |
| topic | R Medicine (General) RA440-440.87 Study and teaching. Research RC Internal medicine |
| url | http://eprints.usm.my/60415/ http://eprints.usm.my/60415/1/NURSYAZANA%20AQILAH%20BINTI%20ALI%20-%20e.pdf |