Purification, Characterization, And Microemulsion Spray Formulation Of Enterocin Secreted By Enterococcus Faeciumcc2 Against Oral Pathogen Streptococcus Mutansukmcc 1019
Dental caries is the most common noncommunicable disease worldwide and Streptococcus mutans is the main oral pathogen which contributes to it. Using of chlorhexidine mouthwash in the inhibition of S. mutans may cause some side effects to human beings and also harmful to aquatic life. Bacteriocin-lik...
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| Format: | Thesis |
| Language: | English |
| Published: |
2023
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| Online Access: | http://eprints.usm.my/60318/ http://eprints.usm.my/60318/1/NG%20ZHANG%20JIN%20-%20TESIS%20cut.pdf |
| _version_ | 1848884413347135488 |
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| author | Ng, Zhang Jin |
| author_facet | Ng, Zhang Jin |
| author_sort | Ng, Zhang Jin |
| building | USM Institutional Repository |
| collection | Online Access |
| description | Dental caries is the most common noncommunicable disease worldwide and Streptococcus mutans is the main oral pathogen which contributes to it. Using of chlorhexidine mouthwash in the inhibition of S. mutans may cause some side effects to human beings and also harmful to aquatic life. Bacteriocin-like inhibitory substance (BLIS) from lactic acid bacteria can be the replacement for chlorhexidine in the inhibition of S. mutans. In this study, out of the 61 LAB strains, the BLIS produced by Enterococcus faecium CC2 showed highest inhibition activity against S. mutans UKMCC 1019. BLIS produced by E. faecium CC2 have high tolerance to acidic, alkaline and high temperature environment. It is a proteinaceous nature substance that possess of 4406.655 AU/mL and 268.592 AU/mL inhibition activity against S. mutans UKMCC 1019 and Candida albicans, respectively. Under TEM, cell lysis of S. mutans UKMCC 1019 was observed. Partial purification of BLIS was done by ammonium sulphate precipitation, following by ionic liquid based aqueous two-phase system (ILATPS) and polyethylene glycol (PEG) based aqueous two-phase system (ATPS), respectively. The highest purification factor, recovery yield and partition coefficient achieved from PEG based ATPS were higher than ILATPS. |
| first_indexed | 2025-11-15T19:06:18Z |
| format | Thesis |
| id | usm-60318 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T19:06:18Z |
| publishDate | 2023 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-603182024-04-01T04:19:53Z http://eprints.usm.my/60318/ Purification, Characterization, And Microemulsion Spray Formulation Of Enterocin Secreted By Enterococcus Faeciumcc2 Against Oral Pathogen Streptococcus Mutansukmcc 1019 Ng, Zhang Jin T1-995 Technology(General) Dental caries is the most common noncommunicable disease worldwide and Streptococcus mutans is the main oral pathogen which contributes to it. Using of chlorhexidine mouthwash in the inhibition of S. mutans may cause some side effects to human beings and also harmful to aquatic life. Bacteriocin-like inhibitory substance (BLIS) from lactic acid bacteria can be the replacement for chlorhexidine in the inhibition of S. mutans. In this study, out of the 61 LAB strains, the BLIS produced by Enterococcus faecium CC2 showed highest inhibition activity against S. mutans UKMCC 1019. BLIS produced by E. faecium CC2 have high tolerance to acidic, alkaline and high temperature environment. It is a proteinaceous nature substance that possess of 4406.655 AU/mL and 268.592 AU/mL inhibition activity against S. mutans UKMCC 1019 and Candida albicans, respectively. Under TEM, cell lysis of S. mutans UKMCC 1019 was observed. Partial purification of BLIS was done by ammonium sulphate precipitation, following by ionic liquid based aqueous two-phase system (ILATPS) and polyethylene glycol (PEG) based aqueous two-phase system (ATPS), respectively. The highest purification factor, recovery yield and partition coefficient achieved from PEG based ATPS were higher than ILATPS. 2023-07 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/60318/1/NG%20ZHANG%20JIN%20-%20TESIS%20cut.pdf Ng, Zhang Jin (2023) Purification, Characterization, And Microemulsion Spray Formulation Of Enterocin Secreted By Enterococcus Faeciumcc2 Against Oral Pathogen Streptococcus Mutansukmcc 1019. PhD thesis, Universiti Sains Malaysia. |
| spellingShingle | T1-995 Technology(General) Ng, Zhang Jin Purification, Characterization, And Microemulsion Spray Formulation Of Enterocin Secreted By Enterococcus Faeciumcc2 Against Oral Pathogen Streptococcus Mutansukmcc 1019 |
| title | Purification, Characterization, And Microemulsion Spray Formulation Of Enterocin Secreted By Enterococcus Faeciumcc2 Against Oral Pathogen Streptococcus Mutansukmcc 1019 |
| title_full | Purification, Characterization, And Microemulsion Spray Formulation Of Enterocin Secreted By Enterococcus Faeciumcc2 Against Oral Pathogen Streptococcus Mutansukmcc 1019 |
| title_fullStr | Purification, Characterization, And Microemulsion Spray Formulation Of Enterocin Secreted By Enterococcus Faeciumcc2 Against Oral Pathogen Streptococcus Mutansukmcc 1019 |
| title_full_unstemmed | Purification, Characterization, And Microemulsion Spray Formulation Of Enterocin Secreted By Enterococcus Faeciumcc2 Against Oral Pathogen Streptococcus Mutansukmcc 1019 |
| title_short | Purification, Characterization, And Microemulsion Spray Formulation Of Enterocin Secreted By Enterococcus Faeciumcc2 Against Oral Pathogen Streptococcus Mutansukmcc 1019 |
| title_sort | purification, characterization, and microemulsion spray formulation of enterocin secreted by enterococcus faeciumcc2 against oral pathogen streptococcus mutansukmcc 1019 |
| topic | T1-995 Technology(General) |
| url | http://eprints.usm.my/60318/ http://eprints.usm.my/60318/1/NG%20ZHANG%20JIN%20-%20TESIS%20cut.pdf |