Modifications Of Nk-92mi Cell Line Through Third Generation Lentiviral Vector
In this study, NK-92MI cell line was transduced with RRL lentivirus carrying the genes for either intracellular GFP or surface CD16 receptor, with the addition of polybrene polycation. Results showed that GFP expression averaged at 3.49 % without polybrene and averaged at 7.18 % when polybrene was a...
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| Format: | Thesis |
| Language: | English |
| Published: |
2023
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| Online Access: | http://eprints.usm.my/60178/ http://eprints.usm.my/60178/1/Pages%20from%20CHIN%20DING%20SHENG%20-%20TESIS.pdf |
| _version_ | 1848884372851130368 |
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| author | Chin, Ding Sheng |
| author_facet | Chin, Ding Sheng |
| author_sort | Chin, Ding Sheng |
| building | USM Institutional Repository |
| collection | Online Access |
| description | In this study, NK-92MI cell line was transduced with RRL lentivirus carrying the genes for either intracellular GFP or surface CD16 receptor, with the addition of polybrene polycation. Results showed that GFP expression averaged at 3.49 % without polybrene and averaged at 7.18 % when polybrene was added. Extracellular CD16 expression averaged at 1.03 % without polybrene and increased to an average of 3.12 % with polybrene. This study demonstrated the feasibility of using the third generation RRL lentiviral vector for NK cell transduction. |
| first_indexed | 2025-11-15T19:05:40Z |
| format | Thesis |
| id | usm-60178 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T19:05:40Z |
| publishDate | 2023 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-601782024-03-14T01:07:38Z http://eprints.usm.my/60178/ Modifications Of Nk-92mi Cell Line Through Third Generation Lentiviral Vector Chin, Ding Sheng R5-130.5 General works In this study, NK-92MI cell line was transduced with RRL lentivirus carrying the genes for either intracellular GFP or surface CD16 receptor, with the addition of polybrene polycation. Results showed that GFP expression averaged at 3.49 % without polybrene and averaged at 7.18 % when polybrene was added. Extracellular CD16 expression averaged at 1.03 % without polybrene and increased to an average of 3.12 % with polybrene. This study demonstrated the feasibility of using the third generation RRL lentiviral vector for NK cell transduction. 2023-01 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/60178/1/Pages%20from%20CHIN%20DING%20SHENG%20-%20TESIS.pdf Chin, Ding Sheng (2023) Modifications Of Nk-92mi Cell Line Through Third Generation Lentiviral Vector. Masters thesis, Universiti Sains Malaysia. |
| spellingShingle | R5-130.5 General works Chin, Ding Sheng Modifications Of Nk-92mi Cell Line Through Third Generation Lentiviral Vector |
| title | Modifications Of Nk-92mi Cell Line Through Third Generation Lentiviral Vector |
| title_full | Modifications Of Nk-92mi Cell Line Through Third Generation Lentiviral Vector |
| title_fullStr | Modifications Of Nk-92mi Cell Line Through Third Generation Lentiviral Vector |
| title_full_unstemmed | Modifications Of Nk-92mi Cell Line Through Third Generation Lentiviral Vector |
| title_short | Modifications Of Nk-92mi Cell Line Through Third Generation Lentiviral Vector |
| title_sort | modifications of nk-92mi cell line through third generation lentiviral vector |
| topic | R5-130.5 General works |
| url | http://eprints.usm.my/60178/ http://eprints.usm.my/60178/1/Pages%20from%20CHIN%20DING%20SHENG%20-%20TESIS.pdf |