Production And Characterization Of Recombinant Monoclonal Antibodies Against Toxocara Antigens
Toxocariasis is a neglected zoonotic parasitic disease caused by intestinal parasitic roundworms of dogs (Toxocara canis) and cats (Toxocara cati). Human toxocariasis has a global distribution and affects mostly people who are socioeconomically deprived. Clinicians face problems in diagnosing human...
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| Format: | Thesis |
| Language: | English |
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2023
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| Online Access: | http://eprints.usm.my/60086/ http://eprints.usm.my/60086/1/ZAMRINA%20BINTI%20MOHD%20BAHARUDEEN%20-%20TESIS%20cut.pdf |
| _version_ | 1848884347289993216 |
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| author | Baharudeen, Zamrina Mohd |
| author_facet | Baharudeen, Zamrina Mohd |
| author_sort | Baharudeen, Zamrina Mohd |
| building | USM Institutional Repository |
| collection | Online Access |
| description | Toxocariasis is a neglected zoonotic parasitic disease caused by intestinal parasitic roundworms of dogs (Toxocara canis) and cats (Toxocara cati). Human toxocariasis has a global distribution and affects mostly people who are socioeconomically deprived. Clinicians face problems in diagnosing human toxocariasis since the signs and symptoms are non-specific and similar to other helminthic infections. Most serodiagnostic methods for toxocariasis detection rely on IgG antibody-based assays using native antigen of T. canis, however the drawback is the lack of high diagnostic specificity due to cross-reactivity with antibodies to other helminths. Additionally, IgG is a long-lasting antibody, thus IgG assays may also detect past (cured) infections. Thus, there is a need to improve the serodiagnosis of toxocariasis and one good way is by developing an antigen detection assay. To develop such an assay, recombinant monoclonal antibodies (rmAbs) to Toxocara excretory-secretory (TES) antigens can be used as the capture reagent. In this study, two recombinant proteins namely T. canis (rTES-26) and T. cati (rTES-120 cati), were expressed and purified. The isolation of mAbs to the recombinant proteins was performed via biopanning using previously produced helminth phage display immune library. Five mAbs against rTES-26 antigen were successfully isolated. However, mAbs against rTES-120 cati antigen showed incomplete scFv sequences; hence, further analysis could not be performed. Henceforth, only the mAbs of the rTES-26 antigen were characterized based on gene family, length of sequence and amino acid distribution. |
| first_indexed | 2025-11-15T19:05:15Z |
| format | Thesis |
| id | usm-60086 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T19:05:15Z |
| publishDate | 2023 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-600862024-03-08T08:06:09Z http://eprints.usm.my/60086/ Production And Characterization Of Recombinant Monoclonal Antibodies Against Toxocara Antigens Baharudeen, Zamrina Mohd R5-920 Medicine (General) Toxocariasis is a neglected zoonotic parasitic disease caused by intestinal parasitic roundworms of dogs (Toxocara canis) and cats (Toxocara cati). Human toxocariasis has a global distribution and affects mostly people who are socioeconomically deprived. Clinicians face problems in diagnosing human toxocariasis since the signs and symptoms are non-specific and similar to other helminthic infections. Most serodiagnostic methods for toxocariasis detection rely on IgG antibody-based assays using native antigen of T. canis, however the drawback is the lack of high diagnostic specificity due to cross-reactivity with antibodies to other helminths. Additionally, IgG is a long-lasting antibody, thus IgG assays may also detect past (cured) infections. Thus, there is a need to improve the serodiagnosis of toxocariasis and one good way is by developing an antigen detection assay. To develop such an assay, recombinant monoclonal antibodies (rmAbs) to Toxocara excretory-secretory (TES) antigens can be used as the capture reagent. In this study, two recombinant proteins namely T. canis (rTES-26) and T. cati (rTES-120 cati), were expressed and purified. The isolation of mAbs to the recombinant proteins was performed via biopanning using previously produced helminth phage display immune library. Five mAbs against rTES-26 antigen were successfully isolated. However, mAbs against rTES-120 cati antigen showed incomplete scFv sequences; hence, further analysis could not be performed. Henceforth, only the mAbs of the rTES-26 antigen were characterized based on gene family, length of sequence and amino acid distribution. 2023-07 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/60086/1/ZAMRINA%20BINTI%20MOHD%20BAHARUDEEN%20-%20TESIS%20cut.pdf Baharudeen, Zamrina Mohd (2023) Production And Characterization Of Recombinant Monoclonal Antibodies Against Toxocara Antigens. Masters thesis, Universiti Sains Malaysia. |
| spellingShingle | R5-920 Medicine (General) Baharudeen, Zamrina Mohd Production And Characterization Of Recombinant Monoclonal Antibodies Against Toxocara Antigens |
| title | Production And Characterization Of Recombinant Monoclonal Antibodies Against Toxocara Antigens |
| title_full | Production And Characterization Of Recombinant Monoclonal Antibodies Against Toxocara Antigens |
| title_fullStr | Production And Characterization Of Recombinant Monoclonal Antibodies Against Toxocara Antigens |
| title_full_unstemmed | Production And Characterization Of Recombinant Monoclonal Antibodies Against Toxocara Antigens |
| title_short | Production And Characterization Of Recombinant Monoclonal Antibodies Against Toxocara Antigens |
| title_sort | production and characterization of recombinant monoclonal antibodies against toxocara antigens |
| topic | R5-920 Medicine (General) |
| url | http://eprints.usm.my/60086/ http://eprints.usm.my/60086/1/ZAMRINA%20BINTI%20MOHD%20BAHARUDEEN%20-%20TESIS%20cut.pdf |