Detection, genotyping & transcriptome analysis of s.typhi & s paratyphi form well water & its association with geographical clustering on enteric fever
A total number of 936 waters samples were collected from the typhoid locality in the year 2011-2013. S. Typhi was isolated from 2 samples whereby one them are from the outbreak cases in Pasir Mas, Kelantan dan the other one is the sporadic cases. Multiplex PCR showed S. Typhi positives in 19 cases...
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| Format: | Monograph |
| Language: | English |
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Pusat Pengajian Kesihatan
2015
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| Online Access: | http://eprints.usm.my/59824/ http://eprints.usm.my/59824/1/DR%20AZIAH%20ISMAIL%20-%20e.pdf |
| _version_ | 1848884275737264128 |
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| author | Ismail, Aziah |
| author_facet | Ismail, Aziah |
| author_sort | Ismail, Aziah |
| building | USM Institutional Repository |
| collection | Online Access |
| description | A total number of 936 waters samples were collected from the typhoid locality in the year 2011-2013. S. Typhi
was isolated from 2 samples whereby one them are from the outbreak cases in Pasir Mas, Kelantan dan the
other one is the sporadic cases. Multiplex PCR showed S. Typhi positives in 19 cases suggesting that the PCR
is more sensitive than the culture method. GIS analysis showed model of relationship between water positivity
and cases. The S. Typhi isolated from water samples from outbreak area was genotyped together with the S.
Typhi isolated from stools of food handlers in the same outbreak area. The pulse-field gel electrophoresis
(PFGE) showed similar pattern with the isolates from well water. The findings suggested that the carriers
among food handlers had contaminated the water and causing the outbreak. A representative S Typhi isolated
from stools of food handlers and one of the S. Typhi isolated from water samples were undergone genome and
RNA sequencing. The results showed that there are several genes that can differentiate between the two
isolates. Transciptome analysis showed that 1272 genes were upregulated when S. Typhi was incubated in well
water compared to the our genome analysis whereas 1065 genes were upregulated compared to CT18 genome
(reference genome sequence in GenBank, NCBI). Further downstream analysis will be continued using other
future grants. |
| first_indexed | 2025-11-15T19:04:07Z |
| format | Monograph |
| id | usm-59824 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T19:04:07Z |
| publishDate | 2015 |
| publisher | Pusat Pengajian Kesihatan |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-598242024-02-13T07:20:23Z http://eprints.usm.my/59824/ Detection, genotyping & transcriptome analysis of s.typhi & s paratyphi form well water & its association with geographical clustering on enteric fever Ismail, Aziah RC109-216 Infectious and parasitic diseases A total number of 936 waters samples were collected from the typhoid locality in the year 2011-2013. S. Typhi was isolated from 2 samples whereby one them are from the outbreak cases in Pasir Mas, Kelantan dan the other one is the sporadic cases. Multiplex PCR showed S. Typhi positives in 19 cases suggesting that the PCR is more sensitive than the culture method. GIS analysis showed model of relationship between water positivity and cases. The S. Typhi isolated from water samples from outbreak area was genotyped together with the S. Typhi isolated from stools of food handlers in the same outbreak area. The pulse-field gel electrophoresis (PFGE) showed similar pattern with the isolates from well water. The findings suggested that the carriers among food handlers had contaminated the water and causing the outbreak. A representative S Typhi isolated from stools of food handlers and one of the S. Typhi isolated from water samples were undergone genome and RNA sequencing. The results showed that there are several genes that can differentiate between the two isolates. Transciptome analysis showed that 1272 genes were upregulated when S. Typhi was incubated in well water compared to the our genome analysis whereas 1065 genes were upregulated compared to CT18 genome (reference genome sequence in GenBank, NCBI). Further downstream analysis will be continued using other future grants. Pusat Pengajian Kesihatan 2015 Monograph NonPeerReviewed application/pdf en http://eprints.usm.my/59824/1/DR%20AZIAH%20ISMAIL%20-%20e.pdf Ismail, Aziah (2015) Detection, genotyping & transcriptome analysis of s.typhi & s paratyphi form well water & its association with geographical clustering on enteric fever. Project Report. Pusat Pengajian Kesihatan. |
| spellingShingle | RC109-216 Infectious and parasitic diseases Ismail, Aziah Detection, genotyping & transcriptome analysis of s.typhi & s paratyphi form well water & its association with geographical clustering on enteric fever |
| title | Detection, genotyping & transcriptome
analysis of s.typhi & s paratyphi form well
water & its association with geographical
clustering on enteric fever |
| title_full | Detection, genotyping & transcriptome
analysis of s.typhi & s paratyphi form well
water & its association with geographical
clustering on enteric fever |
| title_fullStr | Detection, genotyping & transcriptome
analysis of s.typhi & s paratyphi form well
water & its association with geographical
clustering on enteric fever |
| title_full_unstemmed | Detection, genotyping & transcriptome
analysis of s.typhi & s paratyphi form well
water & its association with geographical
clustering on enteric fever |
| title_short | Detection, genotyping & transcriptome
analysis of s.typhi & s paratyphi form well
water & its association with geographical
clustering on enteric fever |
| title_sort | detection, genotyping & transcriptome
analysis of s.typhi & s paratyphi form well
water & its association with geographical
clustering on enteric fever |
| topic | RC109-216 Infectious and parasitic diseases |
| url | http://eprints.usm.my/59824/ http://eprints.usm.my/59824/1/DR%20AZIAH%20ISMAIL%20-%20e.pdf |