Development of integrated multisensor isothermal amplification with nurcleic acid lateral flow system for point of care biosensor assay
1 developed prototype provides a method of definitive detection multiplex for loop-mediated isothermal amplification AMP). Method of amplicons detection relates to a label-based lateral flow dipstick device. The device comprises an orbent pad and a reaction pad. The reaction pad immobilized with m...
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| Format: | Monograph |
| Language: | English |
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Pusat Pengajian Kesihatan
2016
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| Online Access: | http://eprints.usm.my/59771/ http://eprints.usm.my/59771/1/PROF%20MADYA%20DR%20CHAN%20YEA%20YEA%20-%20e.pdf |
| _version_ | 1848884260021207040 |
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| author | Yean, Chan Yean |
| author_facet | Yean, Chan Yean |
| author_sort | Yean, Chan Yean |
| building | USM Institutional Repository |
| collection | Online Access |
| description | 1 developed prototype provides a method of definitive detection multiplex for loop-mediated isothermal amplification
AMP). Method of amplicons detection relates to a label-based lateral flow dipstick device. The device comprises an
orbent pad and a reaction pad. The reaction pad immobilized with more than two sorts of antibodies to detect
tiple dual-labeled LAMP amplicons. A first line on the reaction pad, a chromatography control line, is an
Tunological component that capable of binding to an enzyme-labeled antibody. The second and third lines, known as
■ lines, are also an immunological component that capable of specifically binding to labeled site of LAMP amplicons
ch are another labeled site are bound to enzyme-labeled antibody to form the sandwich complexes. The enzymeeled antibody produces a visual red color by which it is pre-coated with gold nanoparticles. In specific, the present
totype simplifies the multi-steps of mLAMP reagent preparation, reduces the risk of carry-over contamination, ease of
A.MP amplicons detection and improved the credibility of the study. The ready-to-use thermostabilized mLAMP
gents can be potentially applied as a diagnostic kit for diagnosis of diseases on field or in clinical settings. |
| first_indexed | 2025-11-15T19:03:52Z |
| format | Monograph |
| id | usm-59771 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T19:03:52Z |
| publishDate | 2016 |
| publisher | Pusat Pengajian Kesihatan |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-597712024-03-27T07:00:14Z http://eprints.usm.my/59771/ Development of integrated multisensor isothermal amplification with nurcleic acid lateral flow system for point of care biosensor assay Yean, Chan Yean Q Science (General) RC109-216 Infectious and parasitic diseases 1 developed prototype provides a method of definitive detection multiplex for loop-mediated isothermal amplification AMP). Method of amplicons detection relates to a label-based lateral flow dipstick device. The device comprises an orbent pad and a reaction pad. The reaction pad immobilized with more than two sorts of antibodies to detect tiple dual-labeled LAMP amplicons. A first line on the reaction pad, a chromatography control line, is an Tunological component that capable of binding to an enzyme-labeled antibody. The second and third lines, known as ■ lines, are also an immunological component that capable of specifically binding to labeled site of LAMP amplicons ch are another labeled site are bound to enzyme-labeled antibody to form the sandwich complexes. The enzymeeled antibody produces a visual red color by which it is pre-coated with gold nanoparticles. In specific, the present totype simplifies the multi-steps of mLAMP reagent preparation, reduces the risk of carry-over contamination, ease of A.MP amplicons detection and improved the credibility of the study. The ready-to-use thermostabilized mLAMP gents can be potentially applied as a diagnostic kit for diagnosis of diseases on field or in clinical settings. Pusat Pengajian Kesihatan 2016 Monograph NonPeerReviewed application/pdf en http://eprints.usm.my/59771/1/PROF%20MADYA%20DR%20CHAN%20YEA%20YEA%20-%20e.pdf Yean, Chan Yean (2016) Development of integrated multisensor isothermal amplification with nurcleic acid lateral flow system for point of care biosensor assay. Project Report. Pusat Pengajian Kesihatan. (Submitted) |
| spellingShingle | Q Science (General) RC109-216 Infectious and parasitic diseases Yean, Chan Yean Development of integrated multisensor isothermal amplification with nurcleic acid lateral flow system for point of care biosensor assay |
| title | Development of integrated multisensor isothermal
amplification with nurcleic acid lateral flow
system for point of care biosensor assay |
| title_full | Development of integrated multisensor isothermal
amplification with nurcleic acid lateral flow
system for point of care biosensor assay |
| title_fullStr | Development of integrated multisensor isothermal
amplification with nurcleic acid lateral flow
system for point of care biosensor assay |
| title_full_unstemmed | Development of integrated multisensor isothermal
amplification with nurcleic acid lateral flow
system for point of care biosensor assay |
| title_short | Development of integrated multisensor isothermal
amplification with nurcleic acid lateral flow
system for point of care biosensor assay |
| title_sort | development of integrated multisensor isothermal
amplification with nurcleic acid lateral flow
system for point of care biosensor assay |
| topic | Q Science (General) RC109-216 Infectious and parasitic diseases |
| url | http://eprints.usm.my/59771/ http://eprints.usm.my/59771/1/PROF%20MADYA%20DR%20CHAN%20YEA%20YEA%20-%20e.pdf |