Development of integrated multisensor isothermal amplification with nurcleic acid lateral flow system for point of care biosensor assay
1 developed prototype provides a method of definitive detection multiplex for loop-mediated isothermal amplification AMP). Method of amplicons detection relates to a label-based lateral flow dipstick device. The device comprises an orbent pad and a reaction pad. The reaction pad immobilized with m...
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| Format: | Monograph |
| Language: | English |
| Published: |
Pusat Pengajian Kesihatan
2016
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| Online Access: | http://eprints.usm.my/59771/ http://eprints.usm.my/59771/1/PROF%20MADYA%20DR%20CHAN%20YEA%20YEA%20-%20e.pdf |
| Summary: | 1 developed prototype provides a method of definitive detection multiplex for loop-mediated isothermal amplification
AMP). Method of amplicons detection relates to a label-based lateral flow dipstick device. The device comprises an
orbent pad and a reaction pad. The reaction pad immobilized with more than two sorts of antibodies to detect
tiple dual-labeled LAMP amplicons. A first line on the reaction pad, a chromatography control line, is an
Tunological component that capable of binding to an enzyme-labeled antibody. The second and third lines, known as
■ lines, are also an immunological component that capable of specifically binding to labeled site of LAMP amplicons
ch are another labeled site are bound to enzyme-labeled antibody to form the sandwich complexes. The enzymeeled antibody produces a visual red color by which it is pre-coated with gold nanoparticles. In specific, the present
totype simplifies the multi-steps of mLAMP reagent preparation, reduces the risk of carry-over contamination, ease of
A.MP amplicons detection and improved the credibility of the study. The ready-to-use thermostabilized mLAMP
gents can be potentially applied as a diagnostic kit for diagnosis of diseases on field or in clinical settings. |
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