In-vitro & in-vivo study of primary epidermal auto, allo and xenografts at different cell passages transplanted on burn wound in oryctolagus cuniculus
The goal of this study was to investigate the wound healing potential using primary epidermal auto-, allo-, and xenografts in rabbit ( Oryctolagus cuniculus). In this study, rabbit and rat skins were harvested and cultured in vitro. Cultured epithelial autograft, allograft and xenograft cells wer...
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| Format: | Monograph |
| Language: | English |
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Pusat Pengajian Kesihatan
2016
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| Online Access: | http://eprints.usm.my/59767/ http://eprints.usm.my/59767/1/DR%20NURUL%20ASMA%20ABDULLAH%20-%20e.pdf |
| _version_ | 1848884258802761728 |
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| author | Halim, Ahmad Sukari |
| author_facet | Halim, Ahmad Sukari |
| author_sort | Halim, Ahmad Sukari |
| building | USM Institutional Repository |
| collection | Online Access |
| description | The goal of this study was to investigate the wound healing potential using primary
epidermal auto-, allo-, and xenografts in rabbit ( Oryctolagus cuniculus). In this study, rabbit and
rat skins were harvested and cultured in vitro. Cultured epithelial autograft, allograft and
xenograft cells were sprayed onto the three freshly created wounds with one wound acting as a
control. The wounds were monitored every two days for four weeks. After four weeks, the
rabbits were euthanized; skin biopsies were taken from each healed wound and subjected to
hematoxylin and eosin staining followed by immunohistochemical staining using Pancytokeratin,
PCK-26 (Abeam, Germany) and Cytokeratin A1/A3 antibodies. All the examined
grafts showed favorable healing outcomes because the wounds appeared similar to normal skin
upon healing. The only observed difference was related to the thickness of the epidermis layer in
the case of the xenograft transplant, which was thinner compared with the autograft and allograft.
It was also noted that the healing rates of the wounds treated with the xenograft was similar to
wounds treated with autograft and allografts. The level of antigenicity of the 3 grafts appeared to
be the same, which is displayed by the similar healing rate of the grafts. |
| first_indexed | 2025-11-15T19:03:51Z |
| format | Monograph |
| id | usm-59767 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T19:03:51Z |
| publishDate | 2016 |
| publisher | Pusat Pengajian Kesihatan |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-597672024-03-27T06:34:24Z http://eprints.usm.my/59767/ In-vitro & in-vivo study of primary epidermal auto, allo and xenografts at different cell passages transplanted on burn wound in oryctolagus cuniculus Halim, Ahmad Sukari Q Science (General) RC Internal medicine The goal of this study was to investigate the wound healing potential using primary epidermal auto-, allo-, and xenografts in rabbit ( Oryctolagus cuniculus). In this study, rabbit and rat skins were harvested and cultured in vitro. Cultured epithelial autograft, allograft and xenograft cells were sprayed onto the three freshly created wounds with one wound acting as a control. The wounds were monitored every two days for four weeks. After four weeks, the rabbits were euthanized; skin biopsies were taken from each healed wound and subjected to hematoxylin and eosin staining followed by immunohistochemical staining using Pancytokeratin, PCK-26 (Abeam, Germany) and Cytokeratin A1/A3 antibodies. All the examined grafts showed favorable healing outcomes because the wounds appeared similar to normal skin upon healing. The only observed difference was related to the thickness of the epidermis layer in the case of the xenograft transplant, which was thinner compared with the autograft and allograft. It was also noted that the healing rates of the wounds treated with the xenograft was similar to wounds treated with autograft and allografts. The level of antigenicity of the 3 grafts appeared to be the same, which is displayed by the similar healing rate of the grafts. Pusat Pengajian Kesihatan 2016 Monograph NonPeerReviewed application/pdf en http://eprints.usm.my/59767/1/DR%20NURUL%20ASMA%20ABDULLAH%20-%20e.pdf Halim, Ahmad Sukari (2016) In-vitro & in-vivo study of primary epidermal auto, allo and xenografts at different cell passages transplanted on burn wound in oryctolagus cuniculus. Project Report. Pusat Pengajian Kesihatan. (Submitted) |
| spellingShingle | Q Science (General) RC Internal medicine Halim, Ahmad Sukari In-vitro & in-vivo study of primary epidermal auto, allo and xenografts at different cell passages transplanted on burn wound in oryctolagus cuniculus |
| title | In-vitro & in-vivo study of primary epidermal
auto, allo and xenografts at different cell
passages transplanted on burn wound in
oryctolagus cuniculus |
| title_full | In-vitro & in-vivo study of primary epidermal
auto, allo and xenografts at different cell
passages transplanted on burn wound in
oryctolagus cuniculus |
| title_fullStr | In-vitro & in-vivo study of primary epidermal
auto, allo and xenografts at different cell
passages transplanted on burn wound in
oryctolagus cuniculus |
| title_full_unstemmed | In-vitro & in-vivo study of primary epidermal
auto, allo and xenografts at different cell
passages transplanted on burn wound in
oryctolagus cuniculus |
| title_short | In-vitro & in-vivo study of primary epidermal
auto, allo and xenografts at different cell
passages transplanted on burn wound in
oryctolagus cuniculus |
| title_sort | in-vitro & in-vivo study of primary epidermal
auto, allo and xenografts at different cell
passages transplanted on burn wound in
oryctolagus cuniculus |
| topic | Q Science (General) RC Internal medicine |
| url | http://eprints.usm.my/59767/ http://eprints.usm.my/59767/1/DR%20NURUL%20ASMA%20ABDULLAH%20-%20e.pdf |