9th International conference on biosience, biochemistry & bioinformatics 2018
Transient receptor potential cation channel subfamily M member 4 (TRPM4) is overexpressed in activated B-cell-like subtype of diffuse large B-cell lymphoma (ABC-DLBCL) associated with poor survival. In this study, its functions in the disease and the potency of its inhibitor 9-phenanthrol were in...
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| Format: | Monograph |
| Language: | English |
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Pusat Pengajian Perubatan
2019
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| Online Access: | http://eprints.usm.my/59762/ http://eprints.usm.my/59762/1/DR%20WONG%20KAH%20KENG.pdf |
| _version_ | 1848884257718534144 |
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| author | Keng, Wong Kah |
| author_facet | Keng, Wong Kah |
| author_sort | Keng, Wong Kah |
| building | USM Institutional Repository |
| collection | Online Access |
| description | Transient receptor potential cation channel subfamily M member 4 (TRPM4) is
overexpressed in activated B-cell-like subtype of diffuse large B-cell lymphoma (ABC-DLBCL)
associated with poor survival. In this study, its functions in the disease and the potency of its
inhibitor 9-phenanthrol were investigated. The biological functions associated with TR.PM4 mRNA
expression were examined through Gene Set Enrichment Analysis (GSEA) in ABC-DLBCL cases
(n=15). The cytotoxicity of 9-phenanthrol in three ABC-DLBCL cell lines (SUDHL2, OCI-LY3,
OCI-LYIO) was tested at six different concentrations (0.0InM, 0.1 nM, InM, lOnM, 25nM, 50nM).
GSEA results showed that cell cycle gene sets conferred the highest number of gene sets
representing 42% (n=21/50) of the top 50 most significantly enriched gene sets ranked according to
false discovery rate (FDR; all 50 gene sets had FDRO.OI), followed by DNA replication (n=8/50;
16%) and RNA processing (n=8/50; 16%), suggesting the roles of TRPM4 in cell cycle progression
and cellular division of ABC-DLBCL. In terms of the cytotoxicity effects of 9-phenanthrol, the
resulting GI50 for all ABC-DLBCL cell lines ranged from 19nM-41,88nM. In conclusion, TRPM4 is
potentially involved in the cell cycle progression and cellular division of ABC-DLBCL cells, and
the TRPM4 inhibitor 9-phenanthrol was cytotoxic against ABC-DLBCL cells. |
| first_indexed | 2025-11-15T19:03:50Z |
| format | Monograph |
| id | usm-59762 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T19:03:50Z |
| publishDate | 2019 |
| publisher | Pusat Pengajian Perubatan |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-597622024-03-27T06:32:21Z http://eprints.usm.my/59762/ 9th International conference on biosience, biochemistry & bioinformatics 2018 Keng, Wong Kah Q1-390 Science (General) QD Chemistry QD415-436 Biochemistry R5-920 Medicine (General) Transient receptor potential cation channel subfamily M member 4 (TRPM4) is overexpressed in activated B-cell-like subtype of diffuse large B-cell lymphoma (ABC-DLBCL) associated with poor survival. In this study, its functions in the disease and the potency of its inhibitor 9-phenanthrol were investigated. The biological functions associated with TR.PM4 mRNA expression were examined through Gene Set Enrichment Analysis (GSEA) in ABC-DLBCL cases (n=15). The cytotoxicity of 9-phenanthrol in three ABC-DLBCL cell lines (SUDHL2, OCI-LY3, OCI-LYIO) was tested at six different concentrations (0.0InM, 0.1 nM, InM, lOnM, 25nM, 50nM). GSEA results showed that cell cycle gene sets conferred the highest number of gene sets representing 42% (n=21/50) of the top 50 most significantly enriched gene sets ranked according to false discovery rate (FDR; all 50 gene sets had FDRO.OI), followed by DNA replication (n=8/50; 16%) and RNA processing (n=8/50; 16%), suggesting the roles of TRPM4 in cell cycle progression and cellular division of ABC-DLBCL. In terms of the cytotoxicity effects of 9-phenanthrol, the resulting GI50 for all ABC-DLBCL cell lines ranged from 19nM-41,88nM. In conclusion, TRPM4 is potentially involved in the cell cycle progression and cellular division of ABC-DLBCL cells, and the TRPM4 inhibitor 9-phenanthrol was cytotoxic against ABC-DLBCL cells. Pusat Pengajian Perubatan 2019 Monograph NonPeerReviewed application/pdf en http://eprints.usm.my/59762/1/DR%20WONG%20KAH%20KENG.pdf Keng, Wong Kah (2019) 9th International conference on biosience, biochemistry & bioinformatics 2018. Project Report. Pusat Pengajian Perubatan. (Submitted) |
| spellingShingle | Q1-390 Science (General) QD Chemistry QD415-436 Biochemistry R5-920 Medicine (General) Keng, Wong Kah 9th International conference on biosience, biochemistry & bioinformatics 2018 |
| title | 9th International conference on biosience,
biochemistry & bioinformatics 2018 |
| title_full | 9th International conference on biosience,
biochemistry & bioinformatics 2018 |
| title_fullStr | 9th International conference on biosience,
biochemistry & bioinformatics 2018 |
| title_full_unstemmed | 9th International conference on biosience,
biochemistry & bioinformatics 2018 |
| title_short | 9th International conference on biosience,
biochemistry & bioinformatics 2018 |
| title_sort | 9th international conference on biosience,
biochemistry & bioinformatics 2018 |
| topic | Q1-390 Science (General) QD Chemistry QD415-436 Biochemistry R5-920 Medicine (General) |
| url | http://eprints.usm.my/59762/ http://eprints.usm.my/59762/1/DR%20WONG%20KAH%20KENG.pdf |