Characterization Of Astaxanthinrich Xanthophyllomyces Dendrorhous Extract From A Hyperproducing Mutant And Its Effects On Breast Cancer Cells
Astaxanthin is a carotenoid with multiple health benefits including antioxidant and anticancer properties. An astaxanthin-hyperproducing X. dendrorhous mutant was generated due to the growing preference for natural pigment. This work aimed to study the astaxanthin-rich X. dendrorhous extracts and...
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| Format: | Thesis |
| Language: | English |
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2022
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| Online Access: | http://eprints.usm.my/59551/ http://eprints.usm.my/59551/1/24%20Pages%20from%20KHAW%20SHIN%20YUAN.pdf |
| _version_ | 1848884202622156800 |
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| author | Khaw, Shin Yuan |
| author_facet | Khaw, Shin Yuan |
| author_sort | Khaw, Shin Yuan |
| building | USM Institutional Repository |
| collection | Online Access |
| description | Astaxanthin is a carotenoid with multiple health benefits including antioxidant
and anticancer properties. An astaxanthin-hyperproducing X. dendrorhous mutant was
generated due to the growing preference for natural pigment. This work aimed to study
the astaxanthin-rich X. dendrorhous extracts and its effect on MCF-7 and MDA-MB-
231 cells. Spectrometric, TLC and HPLC analysis evidenced that mutant M34
produced astaxanthin in higher quantity and purity than the wild type. Mutant
astaxanthin extract showed a higher scavenging activity compared to wild type
astaxanthin extract in DPPH assay. MTT assays proved that wild type and M34
astaxanthin extracts were not toxic towards the non-cancer origin MCF-10A cells.
Wild type and mutant astaxanthin extracts exhibit growth-inhibitory effect in dosedependent
manner in MCF-7 and MDA-MB-231 cells. Mutant astaxanthin extract
showed a lower IC50 than wild type extract and was more effective in inhibiting both
MCF-7 and MDA-MB-231 cells. The IC50 values of wild type and mutant astaxanthin
extracts did not exceed the required upper limit of 30 μg/ml. |
| first_indexed | 2025-11-15T19:02:58Z |
| format | Thesis |
| id | usm-59551 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T19:02:58Z |
| publishDate | 2022 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-595512023-10-26T02:25:44Z http://eprints.usm.my/59551/ Characterization Of Astaxanthinrich Xanthophyllomyces Dendrorhous Extract From A Hyperproducing Mutant And Its Effects On Breast Cancer Cells Khaw, Shin Yuan R5-920 Medicine (General) Astaxanthin is a carotenoid with multiple health benefits including antioxidant and anticancer properties. An astaxanthin-hyperproducing X. dendrorhous mutant was generated due to the growing preference for natural pigment. This work aimed to study the astaxanthin-rich X. dendrorhous extracts and its effect on MCF-7 and MDA-MB- 231 cells. Spectrometric, TLC and HPLC analysis evidenced that mutant M34 produced astaxanthin in higher quantity and purity than the wild type. Mutant astaxanthin extract showed a higher scavenging activity compared to wild type astaxanthin extract in DPPH assay. MTT assays proved that wild type and M34 astaxanthin extracts were not toxic towards the non-cancer origin MCF-10A cells. Wild type and mutant astaxanthin extracts exhibit growth-inhibitory effect in dosedependent manner in MCF-7 and MDA-MB-231 cells. Mutant astaxanthin extract showed a lower IC50 than wild type extract and was more effective in inhibiting both MCF-7 and MDA-MB-231 cells. The IC50 values of wild type and mutant astaxanthin extracts did not exceed the required upper limit of 30 μg/ml. 2022-12 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/59551/1/24%20Pages%20from%20KHAW%20SHIN%20YUAN.pdf Khaw, Shin Yuan (2022) Characterization Of Astaxanthinrich Xanthophyllomyces Dendrorhous Extract From A Hyperproducing Mutant And Its Effects On Breast Cancer Cells. Masters thesis, Perpustakaan Hamzah Sendut. |
| spellingShingle | R5-920 Medicine (General) Khaw, Shin Yuan Characterization Of Astaxanthinrich Xanthophyllomyces Dendrorhous Extract From A Hyperproducing Mutant And Its Effects On Breast Cancer Cells |
| title | Characterization Of Astaxanthinrich Xanthophyllomyces Dendrorhous Extract From A Hyperproducing Mutant And Its Effects On Breast Cancer Cells |
| title_full | Characterization Of Astaxanthinrich Xanthophyllomyces Dendrorhous Extract From A Hyperproducing Mutant And Its Effects On Breast Cancer Cells |
| title_fullStr | Characterization Of Astaxanthinrich Xanthophyllomyces Dendrorhous Extract From A Hyperproducing Mutant And Its Effects On Breast Cancer Cells |
| title_full_unstemmed | Characterization Of Astaxanthinrich Xanthophyllomyces Dendrorhous Extract From A Hyperproducing Mutant And Its Effects On Breast Cancer Cells |
| title_short | Characterization Of Astaxanthinrich Xanthophyllomyces Dendrorhous Extract From A Hyperproducing Mutant And Its Effects On Breast Cancer Cells |
| title_sort | characterization of astaxanthinrich xanthophyllomyces dendrorhous extract from a hyperproducing mutant and its effects on breast cancer cells |
| topic | R5-920 Medicine (General) |
| url | http://eprints.usm.my/59551/ http://eprints.usm.my/59551/1/24%20Pages%20from%20KHAW%20SHIN%20YUAN.pdf |