Molecular Characterization of Fish Species Using Mitochondrial DNA 12S rRNA.
This study was undertaken to study the capability of a set of new primers (unpublished) to amplify partial sequence of mitochondrial DNA (mtDNA) 12S rRNA gene of marine and freshwater fish species. The primers namely Forward: 5’ - CAA ACT GGG ATT AGA TAC CCC AC - 3’, and Reverse: 5’ - TAC RCT TA...
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| Format: | Monograph |
| Language: | English |
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Universiti Sains Malaysia
2015
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| Online Access: | http://eprints.usm.my/59081/ http://eprints.usm.my/59081/1/FATIN%20SOLEHA%20BINTI%20FERDAUS%20-%20e24.pdf |
| Summary: | This study was undertaken to study the capability of a set of new primers
(unpublished) to amplify partial sequence of mitochondrial DNA (mtDNA) 12S
rRNA gene of marine and freshwater fish species. The primers namely Forward:
5’ - CAA ACT GGG ATT AGA TAC CCC AC - 3’, and Reverse: 5’ - TAC RCT
TAC CAT GTT ACG ACT T - 3’ were able to amplify the seventeen fish species
chosen for this study. Pairwise comparison of the sequence data of the 17 fish
species were undertaken to study the intra-species variations in the partial
sequence of mtDNA 12S rRNA gene. The Thunnus tonggol and Scomberomorus
commerson showed the highest similarity that is 89.37%. Diagramma pictum and
Siganus canaliculatus showed the second highest similarity with 86.26%. The
lowest similarity seen between Leptobarbus hoevenii and Nemipterus bathybius
with 67.77%.
A total of 1354 nucleotide positional polymorphisms were recorded. The
transition (T<->C and A<->G) 628 out of 1354 and the number of transversion
was 514. The lowest number of nucleotide polymorphism was observed in
Siganus canaliculatus 8.3% and Nemipterus bathybius has showed the highest
nucleotide polymorphism with 26.81%. An unrooted neighbor joining tree (NJ) was
constructed to study their genetic relationship. |
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