The evaluation of program death 1 and PD-1 ligand expressions in histological subtypes of primary extranodal non hodgkin lymphoma

Introduction: Emergence of Programmed death 1 (PD-1) and its ligands, PD-L1 immunotherapy provide new insight in the treatment modality of malignancies. The responsiveness of the patients are associated with the expressions of the PD-L1 and PD-1 protein. We aimed to evaluate the expression of PD-...

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Main Author: Mohaidin, Farhana Mohammad
Format: Thesis
Language:English
Published: 2021
Subjects:
Online Access:http://eprints.usm.my/58572/
http://eprints.usm.my/58572/1/FARHANA%20MOHAMMAD%20MOHAIDIN-24%20pages.pdf
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author Mohaidin, Farhana Mohammad
author_facet Mohaidin, Farhana Mohammad
author_sort Mohaidin, Farhana Mohammad
building USM Institutional Repository
collection Online Access
description Introduction: Emergence of Programmed death 1 (PD-1) and its ligands, PD-L1 immunotherapy provide new insight in the treatment modality of malignancies. The responsiveness of the patients are associated with the expressions of the PD-L1 and PD-1 protein. We aimed to evaluate the expression of PD-1 in the tumour microenvironment and PD-L1 in the tumour cells with histological subtypes of primary extranodal non-Hodgkin lymphoma (peNHL). Methodology: A retrospective cross-sectional study using 87 archived formalin fixed paraffin-embedded tissue blocks of patients diagnosed with peNHL. Samples were stained for PD-1 and PD-L1 by immunohistochemistry method and the proteins expressions were evaluated microscopically. The association between expression of the PD-1 and PD-L1 with subtypes of peNHL were statistically analysed. Results: Majority of the cases are negative expression of PD-L1 in the tumour cells (46, 52.9%), however majority are positive for PD-1 expression in the tumour microenvironment (57, 65.5%). Significant associations were found between PD-1 and PD-L1 expression with subtypes of peNHL (p<0.05) and higher expression was found in DLBCL. Conclusion: Our study, the first in Malaysia to explore expression of PD-1 and PD-L1 in peNHL, demonstrates significant association of PD-1 and PD-L1 expressions with subtypes of peNHL which may impact treatment of these patients in the future.
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spelling usm-585722023-05-18T02:28:28Z http://eprints.usm.my/58572/ The evaluation of program death 1 and PD-1 ligand expressions in histological subtypes of primary extranodal non hodgkin lymphoma Mohaidin, Farhana Mohammad RC Internal medicine Introduction: Emergence of Programmed death 1 (PD-1) and its ligands, PD-L1 immunotherapy provide new insight in the treatment modality of malignancies. The responsiveness of the patients are associated with the expressions of the PD-L1 and PD-1 protein. We aimed to evaluate the expression of PD-1 in the tumour microenvironment and PD-L1 in the tumour cells with histological subtypes of primary extranodal non-Hodgkin lymphoma (peNHL). Methodology: A retrospective cross-sectional study using 87 archived formalin fixed paraffin-embedded tissue blocks of patients diagnosed with peNHL. Samples were stained for PD-1 and PD-L1 by immunohistochemistry method and the proteins expressions were evaluated microscopically. The association between expression of the PD-1 and PD-L1 with subtypes of peNHL were statistically analysed. Results: Majority of the cases are negative expression of PD-L1 in the tumour cells (46, 52.9%), however majority are positive for PD-1 expression in the tumour microenvironment (57, 65.5%). Significant associations were found between PD-1 and PD-L1 expression with subtypes of peNHL (p<0.05) and higher expression was found in DLBCL. Conclusion: Our study, the first in Malaysia to explore expression of PD-1 and PD-L1 in peNHL, demonstrates significant association of PD-1 and PD-L1 expressions with subtypes of peNHL which may impact treatment of these patients in the future. 2021 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/58572/1/FARHANA%20MOHAMMAD%20MOHAIDIN-24%20pages.pdf Mohaidin, Farhana Mohammad (2021) The evaluation of program death 1 and PD-1 ligand expressions in histological subtypes of primary extranodal non hodgkin lymphoma. Masters thesis, Universiti Sains Malaysia.
spellingShingle RC Internal medicine
Mohaidin, Farhana Mohammad
The evaluation of program death 1 and PD-1 ligand expressions in histological subtypes of primary extranodal non hodgkin lymphoma
title The evaluation of program death 1 and PD-1 ligand expressions in histological subtypes of primary extranodal non hodgkin lymphoma
title_full The evaluation of program death 1 and PD-1 ligand expressions in histological subtypes of primary extranodal non hodgkin lymphoma
title_fullStr The evaluation of program death 1 and PD-1 ligand expressions in histological subtypes of primary extranodal non hodgkin lymphoma
title_full_unstemmed The evaluation of program death 1 and PD-1 ligand expressions in histological subtypes of primary extranodal non hodgkin lymphoma
title_short The evaluation of program death 1 and PD-1 ligand expressions in histological subtypes of primary extranodal non hodgkin lymphoma
title_sort evaluation of program death 1 and pd-1 ligand expressions in histological subtypes of primary extranodal non hodgkin lymphoma
topic RC Internal medicine
url http://eprints.usm.my/58572/
http://eprints.usm.my/58572/1/FARHANA%20MOHAMMAD%20MOHAIDIN-24%20pages.pdf