Methanolic extract of Abrus precatorius promotes breast cancer MDA-MB-231 cell death by inducing cell cycle arrest at G0/G1 and upregulating Bax
Objective: To determine the anti-proliferative activity of Abrus precatorius (A. precatorius) leaf extracts and their effect on cell death. Methods: A. precatorius leaves were extracted successively with hexane, ethyl acetate and methanol by Soxhlet extraction. Aqueous extract was prepared by dec...
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| Format: | Article |
| Language: | English |
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Elsevier
2019
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| Online Access: | http://eprints.usm.my/57031/ http://eprints.usm.my/57031/1/Wan%20Suriyani%20Wan%20Ibrahim-Eprints.pdf |
| _version_ | 1848883515527004160 |
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| author | Ibrahim, Wan Suriyani Wan |
| author_facet | Ibrahim, Wan Suriyani Wan |
| author_sort | Ibrahim, Wan Suriyani Wan |
| building | USM Institutional Repository |
| collection | Online Access |
| description | Objective: To determine the anti-proliferative activity of Abrus precatorius (A. precatorius) leaf
extracts and their effect on cell death.
Methods: A. precatorius leaves were extracted successively with hexane, ethyl acetate
and methanol by Soxhlet extraction. Aqueous extract was prepared by decoction at 50 .
Extracts of A. precatorius leaves were used to treat selected cancer and normal cell lines for
72 h. Furthermore, 3-(4,5-dimethyl thiazol-2-yl) 2,5-diphenyl tetrazolium bromide assay
was performed to determine cell viability. Analysis of cell cycle arrest, apoptosis assay and
apoptosis protein expressions were determined by flow cytometry.
Results: Methanolic extract of A. precatorius leaves showed the lowest IC50 on MDA-MB-231
cells at (26.40依5.40) μg/mL. Flow cytometry analysis revealed that cell arrest occurred at G0/
G1 phase and the apoptosis assay showed the occurrence of early apoptosis at 48 h in MDAMB-
231 cells treated with methanolic extract of A. precatorius leaves. Methanolic extract
of A. precatorius leaves induced apoptosis by upregulation of Bax, p53 and caspase-3 and
downregulation of Bcl-2.
Conclusions: Methanolic extract of A. precatorius leaves promotes MDA-MB-231 cell death
by inducing cell cycle arrest and apoptosis possibly via the mitochondrial-related pathway. |
| first_indexed | 2025-11-15T18:52:02Z |
| format | Article |
| id | usm-57031 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T18:52:02Z |
| publishDate | 2019 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-570312023-03-01T08:08:36Z http://eprints.usm.my/57031/ Methanolic extract of Abrus precatorius promotes breast cancer MDA-MB-231 cell death by inducing cell cycle arrest at G0/G1 and upregulating Bax Ibrahim, Wan Suriyani Wan R Medicine RC254-282 Neoplasms. Tumors. Oncology (including Cancer) Objective: To determine the anti-proliferative activity of Abrus precatorius (A. precatorius) leaf extracts and their effect on cell death. Methods: A. precatorius leaves were extracted successively with hexane, ethyl acetate and methanol by Soxhlet extraction. Aqueous extract was prepared by decoction at 50 . Extracts of A. precatorius leaves were used to treat selected cancer and normal cell lines for 72 h. Furthermore, 3-(4,5-dimethyl thiazol-2-yl) 2,5-diphenyl tetrazolium bromide assay was performed to determine cell viability. Analysis of cell cycle arrest, apoptosis assay and apoptosis protein expressions were determined by flow cytometry. Results: Methanolic extract of A. precatorius leaves showed the lowest IC50 on MDA-MB-231 cells at (26.40依5.40) μg/mL. Flow cytometry analysis revealed that cell arrest occurred at G0/ G1 phase and the apoptosis assay showed the occurrence of early apoptosis at 48 h in MDAMB- 231 cells treated with methanolic extract of A. precatorius leaves. Methanolic extract of A. precatorius leaves induced apoptosis by upregulation of Bax, p53 and caspase-3 and downregulation of Bcl-2. Conclusions: Methanolic extract of A. precatorius leaves promotes MDA-MB-231 cell death by inducing cell cycle arrest and apoptosis possibly via the mitochondrial-related pathway. Elsevier 2019 Article NonPeerReviewed application/pdf en http://eprints.usm.my/57031/1/Wan%20Suriyani%20Wan%20Ibrahim-Eprints.pdf Ibrahim, Wan Suriyani Wan (2019) Methanolic extract of Abrus precatorius promotes breast cancer MDA-MB-231 cell death by inducing cell cycle arrest at G0/G1 and upregulating Bax. Asian Pacific Journal of Tropical Biomedicine, 9 (6). pp. 249-256. ISSN 2221-1691 (Submitted) |
| spellingShingle | R Medicine RC254-282 Neoplasms. Tumors. Oncology (including Cancer) Ibrahim, Wan Suriyani Wan Methanolic extract of Abrus precatorius promotes breast cancer MDA-MB-231 cell death by inducing cell cycle arrest at G0/G1 and upregulating Bax |
| title | Methanolic extract of Abrus precatorius promotes breast cancer MDA-MB-231 cell
death by inducing cell cycle arrest at G0/G1 and upregulating Bax |
| title_full | Methanolic extract of Abrus precatorius promotes breast cancer MDA-MB-231 cell
death by inducing cell cycle arrest at G0/G1 and upregulating Bax |
| title_fullStr | Methanolic extract of Abrus precatorius promotes breast cancer MDA-MB-231 cell
death by inducing cell cycle arrest at G0/G1 and upregulating Bax |
| title_full_unstemmed | Methanolic extract of Abrus precatorius promotes breast cancer MDA-MB-231 cell
death by inducing cell cycle arrest at G0/G1 and upregulating Bax |
| title_short | Methanolic extract of Abrus precatorius promotes breast cancer MDA-MB-231 cell
death by inducing cell cycle arrest at G0/G1 and upregulating Bax |
| title_sort | methanolic extract of abrus precatorius promotes breast cancer mda-mb-231 cell
death by inducing cell cycle arrest at g0/g1 and upregulating bax |
| topic | R Medicine RC254-282 Neoplasms. Tumors. Oncology (including Cancer) |
| url | http://eprints.usm.my/57031/ http://eprints.usm.my/57031/1/Wan%20Suriyani%20Wan%20Ibrahim-Eprints.pdf |