Morphological, Pathogenic, Genetic And Molecular Variabilities Of Fusarium Spp., The Pathogens Of Asparagus Crown And Root Rot In Malaysia And Brunei Darussalam

Asparagus (Asparagus officinalis) is becoming more important in South East Asia (SEA) and quickly becoming a preferred vegetable. All varieties planted throughout SEA have been and now are still facing the most destructive disease i.e. asparagus crown and root rot. The main aim of the thesis was...

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Main Author: Al-Amodi, Mohamed Othman Saeed
Format: Thesis
Language:English
Published: 2006
Subjects:
Online Access:http://eprints.usm.my/56227/
http://eprints.usm.my/56227/1/00001648347%20MOSA-A.pdf
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author Al-Amodi, Mohamed Othman Saeed
author_facet Al-Amodi, Mohamed Othman Saeed
author_sort Al-Amodi, Mohamed Othman Saeed
building USM Institutional Repository
collection Online Access
description Asparagus (Asparagus officinalis) is becoming more important in South East Asia (SEA) and quickly becoming a preferred vegetable. All varieties planted throughout SEA have been and now are still facing the most destructive disease i.e. asparagus crown and root rot. The main aim of the thesis was to collect and isolate Fusarium spp. from asparagus plants showing crown and root rot symptoms and their soils in Malaysia and Brunei Darussalam. The other objectives were to evaluate pathogenicity and genetic diversity within the Fusarium spp. and their variability using peR-based RAPD. A total of 110 isolates comprising five species of Fusarium have been isolated from six sampling areas in Malaysia and one in Brunei Darussalam. The five species identified were F. proliferatum, F. oxysporum, F. solani, F. semitectum and F. /ongipes, based on morphological characteristics. F. proliteretum and F. oxysporum represented the highest percentage (830/0). Pathogenicity tests of F. proiiteretum and F. oxysporum isolates by inoculating healthy asparagus seedlings var. UC157 in the greenhouse confirmed that all isolates tested were pathogenic. The typical symptoms were initially observed as yellowing of leaves and branches. Infected plants were stunted with reddishbrown discoloration and shrivelled roots. Sliced crowns and stems clearly showed reddish-brown discolorations of the infected tissues. Heavily infected plants collapsed and died.
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format Thesis
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institution Universiti Sains Malaysia
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language English
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publishDate 2006
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spelling usm-562272023-01-05T07:37:28Z http://eprints.usm.my/56227/ Morphological, Pathogenic, Genetic And Molecular Variabilities Of Fusarium Spp., The Pathogens Of Asparagus Crown And Root Rot In Malaysia And Brunei Darussalam Al-Amodi, Mohamed Othman Saeed QH1-278.5 Natural history (General) Asparagus (Asparagus officinalis) is becoming more important in South East Asia (SEA) and quickly becoming a preferred vegetable. All varieties planted throughout SEA have been and now are still facing the most destructive disease i.e. asparagus crown and root rot. The main aim of the thesis was to collect and isolate Fusarium spp. from asparagus plants showing crown and root rot symptoms and their soils in Malaysia and Brunei Darussalam. The other objectives were to evaluate pathogenicity and genetic diversity within the Fusarium spp. and their variability using peR-based RAPD. A total of 110 isolates comprising five species of Fusarium have been isolated from six sampling areas in Malaysia and one in Brunei Darussalam. The five species identified were F. proliferatum, F. oxysporum, F. solani, F. semitectum and F. /ongipes, based on morphological characteristics. F. proliteretum and F. oxysporum represented the highest percentage (830/0). Pathogenicity tests of F. proiiteretum and F. oxysporum isolates by inoculating healthy asparagus seedlings var. UC157 in the greenhouse confirmed that all isolates tested were pathogenic. The typical symptoms were initially observed as yellowing of leaves and branches. Infected plants were stunted with reddishbrown discoloration and shrivelled roots. Sliced crowns and stems clearly showed reddish-brown discolorations of the infected tissues. Heavily infected plants collapsed and died. 2006-04 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/56227/1/00001648347%20MOSA-A.pdf Al-Amodi, Mohamed Othman Saeed (2006) Morphological, Pathogenic, Genetic And Molecular Variabilities Of Fusarium Spp., The Pathogens Of Asparagus Crown And Root Rot In Malaysia And Brunei Darussalam. PhD thesis, Perpustakaan Hamzah Sendut.
spellingShingle QH1-278.5 Natural history (General)
Al-Amodi, Mohamed Othman Saeed
Morphological, Pathogenic, Genetic And Molecular Variabilities Of Fusarium Spp., The Pathogens Of Asparagus Crown And Root Rot In Malaysia And Brunei Darussalam
title Morphological, Pathogenic, Genetic And Molecular Variabilities Of Fusarium Spp., The Pathogens Of Asparagus Crown And Root Rot In Malaysia And Brunei Darussalam
title_full Morphological, Pathogenic, Genetic And Molecular Variabilities Of Fusarium Spp., The Pathogens Of Asparagus Crown And Root Rot In Malaysia And Brunei Darussalam
title_fullStr Morphological, Pathogenic, Genetic And Molecular Variabilities Of Fusarium Spp., The Pathogens Of Asparagus Crown And Root Rot In Malaysia And Brunei Darussalam
title_full_unstemmed Morphological, Pathogenic, Genetic And Molecular Variabilities Of Fusarium Spp., The Pathogens Of Asparagus Crown And Root Rot In Malaysia And Brunei Darussalam
title_short Morphological, Pathogenic, Genetic And Molecular Variabilities Of Fusarium Spp., The Pathogens Of Asparagus Crown And Root Rot In Malaysia And Brunei Darussalam
title_sort morphological, pathogenic, genetic and molecular variabilities of fusarium spp., the pathogens of asparagus crown and root rot in malaysia and brunei darussalam
topic QH1-278.5 Natural history (General)
url http://eprints.usm.my/56227/
http://eprints.usm.my/56227/1/00001648347%20MOSA-A.pdf