Synthesis Of Zinc Oxide Nanoparticles Using Gallic Acid Characterization And Evaluation Of Antioxidant Activity
Zinc oxide (ZnO) nanoparticles are widely used in biomedical industries due to their antioxidant, anti-cancer, anti-inflammatory, antibacterial and antifungal properties. The synthesis of ZnO nanoparticles via the green synthesis pathway has become the focus of the researcher over the past years due...
| Main Author: | |
|---|---|
| Format: | Monograph |
| Language: | English |
| Published: |
Universiti Sains Malaysia
2022
|
| Subjects: | |
| Online Access: | http://eprints.usm.my/55709/ http://eprints.usm.my/55709/1/Synthesis%20Of%20Zinc%20Oxide%20Nanoparticles%20Using%20Gallic%20Acid%20Characterization%20And%20Evaluation%20Of%20Antioxidant%20Activity.pdf |
| _version_ | 1848883156759871488 |
|---|---|
| author | Kabeer, Saiful Munir Sultan Syed Ahmad |
| author_facet | Kabeer, Saiful Munir Sultan Syed Ahmad |
| author_sort | Kabeer, Saiful Munir Sultan Syed Ahmad |
| building | USM Institutional Repository |
| collection | Online Access |
| description | Zinc oxide (ZnO) nanoparticles are widely used in biomedical industries due to their antioxidant, anti-cancer, anti-inflammatory, antibacterial and antifungal properties. The synthesis of ZnO nanoparticles via the green synthesis pathway has become the focus of the researcher over the past years due to its being environmentally benign, less harmful and non-toxic. In this present study, the synthesis of ZnO nanoparticles via a chemically green synthesis pathway using the chemical precipitation technique was done. A precursor of zinc acetate dihydrate and gallic acid as a reducing and capping agent was used. The effect of varying the pH of solutions from 7 to 11 was studied to synthesize ZnO nanoparticles. Besides, the molar ratios of gallic acid to zinc acetate dihydrate were also studied by changing from 1.0% to 4.0% during the synthesis process. The synthesized ZnO nanoparticles were characterized under different techniques, including Ultraviolet-visible (UV-Vis) spectroscopy, Fourier-transform infrared spectroscopy (FT-IR), Energy-dispersive X-ray spectroscopy (EDX) and Scanning electron microscope (SEM). The morphology and particle size were observed using SEM under both synthesis conditions. The particle sizes were estimated at 269.65 ± 8.23 nm, 234.91 ± 7.51 nm and 118.76 ± 1.27 nm for pH 9, 10 and 11, respectively. The results illustrated that the synthesis under pH 7 and 8 led to agglomeration. Moreover, the particle sizes were estimated at 118.76 ± 1.27 nm, 201.85 ± 16.06 nm, 198.49 ± 15.25 nm, 174.86 ± 6.93 nm, and 99.70 ± 2.78 nm for various molar ratio gallic acid to zinc
precursor 1.0, 1.5 3.0, 3.5 and 4.0%, respectively. The results also revealed that varying molar ratios had influenced the different types of shapes of ZnO nanoparticles. Finally, the optimum from both synthesis conditions (pH 11 and GA: Zn of 4.0%) was used to study antioxidant activity. The ZnO nanoparticles exhibited good antioxidant activity, with IC50 values of 28.88 μg/ml and 25.40 μg/ml for DPPH and ABTS, respectively. This suggested that ZnO nanoparticles could have potential uses in biomedical fields and act as an abiotic elicitor to enhance the biosynthesis of secondary metabolites in "in vitro" plant cell cultures. |
| first_indexed | 2025-11-15T18:46:20Z |
| format | Monograph |
| id | usm-55709 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T18:46:20Z |
| publishDate | 2022 |
| publisher | Universiti Sains Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-557092022-11-21T08:24:17Z http://eprints.usm.my/55709/ Synthesis Of Zinc Oxide Nanoparticles Using Gallic Acid Characterization And Evaluation Of Antioxidant Activity Kabeer, Saiful Munir Sultan Syed Ahmad T Technology TP155-156 Chemical engineering Zinc oxide (ZnO) nanoparticles are widely used in biomedical industries due to their antioxidant, anti-cancer, anti-inflammatory, antibacterial and antifungal properties. The synthesis of ZnO nanoparticles via the green synthesis pathway has become the focus of the researcher over the past years due to its being environmentally benign, less harmful and non-toxic. In this present study, the synthesis of ZnO nanoparticles via a chemically green synthesis pathway using the chemical precipitation technique was done. A precursor of zinc acetate dihydrate and gallic acid as a reducing and capping agent was used. The effect of varying the pH of solutions from 7 to 11 was studied to synthesize ZnO nanoparticles. Besides, the molar ratios of gallic acid to zinc acetate dihydrate were also studied by changing from 1.0% to 4.0% during the synthesis process. The synthesized ZnO nanoparticles were characterized under different techniques, including Ultraviolet-visible (UV-Vis) spectroscopy, Fourier-transform infrared spectroscopy (FT-IR), Energy-dispersive X-ray spectroscopy (EDX) and Scanning electron microscope (SEM). The morphology and particle size were observed using SEM under both synthesis conditions. The particle sizes were estimated at 269.65 ± 8.23 nm, 234.91 ± 7.51 nm and 118.76 ± 1.27 nm for pH 9, 10 and 11, respectively. The results illustrated that the synthesis under pH 7 and 8 led to agglomeration. Moreover, the particle sizes were estimated at 118.76 ± 1.27 nm, 201.85 ± 16.06 nm, 198.49 ± 15.25 nm, 174.86 ± 6.93 nm, and 99.70 ± 2.78 nm for various molar ratio gallic acid to zinc precursor 1.0, 1.5 3.0, 3.5 and 4.0%, respectively. The results also revealed that varying molar ratios had influenced the different types of shapes of ZnO nanoparticles. Finally, the optimum from both synthesis conditions (pH 11 and GA: Zn of 4.0%) was used to study antioxidant activity. The ZnO nanoparticles exhibited good antioxidant activity, with IC50 values of 28.88 μg/ml and 25.40 μg/ml for DPPH and ABTS, respectively. This suggested that ZnO nanoparticles could have potential uses in biomedical fields and act as an abiotic elicitor to enhance the biosynthesis of secondary metabolites in "in vitro" plant cell cultures. Universiti Sains Malaysia 2022-07-01 Monograph NonPeerReviewed application/pdf en http://eprints.usm.my/55709/1/Synthesis%20Of%20Zinc%20Oxide%20Nanoparticles%20Using%20Gallic%20Acid%20Characterization%20And%20Evaluation%20Of%20Antioxidant%20Activity.pdf Kabeer, Saiful Munir Sultan Syed Ahmad (2022) Synthesis Of Zinc Oxide Nanoparticles Using Gallic Acid Characterization And Evaluation Of Antioxidant Activity. Project Report. Universiti Sains Malaysia, Pusat Pengajian Kejuruteraan Kimia. (Submitted) |
| spellingShingle | T Technology TP155-156 Chemical engineering Kabeer, Saiful Munir Sultan Syed Ahmad Synthesis Of Zinc Oxide Nanoparticles Using Gallic Acid Characterization And Evaluation Of Antioxidant Activity |
| title | Synthesis Of Zinc Oxide Nanoparticles Using Gallic Acid Characterization And Evaluation Of Antioxidant Activity |
| title_full | Synthesis Of Zinc Oxide Nanoparticles Using Gallic Acid Characterization And Evaluation Of Antioxidant Activity |
| title_fullStr | Synthesis Of Zinc Oxide Nanoparticles Using Gallic Acid Characterization And Evaluation Of Antioxidant Activity |
| title_full_unstemmed | Synthesis Of Zinc Oxide Nanoparticles Using Gallic Acid Characterization And Evaluation Of Antioxidant Activity |
| title_short | Synthesis Of Zinc Oxide Nanoparticles Using Gallic Acid Characterization And Evaluation Of Antioxidant Activity |
| title_sort | synthesis of zinc oxide nanoparticles using gallic acid characterization and evaluation of antioxidant activity |
| topic | T Technology TP155-156 Chemical engineering |
| url | http://eprints.usm.my/55709/ http://eprints.usm.my/55709/1/Synthesis%20Of%20Zinc%20Oxide%20Nanoparticles%20Using%20Gallic%20Acid%20Characterization%20And%20Evaluation%20Of%20Antioxidant%20Activity.pdf |