The expression of ppars gamma in the cervical cancer line, bela and the effects of specific PPAR ligand on cell growth

To quantify the mRNA expression levels of PPARy in the cervical cancer cell line, HeLa, using real-time PCR. To correlate the findings in (1) with the corresponding protein expression levels by Western blotting. To correlate the transcriptional and translational levels of PPARy with markers of...

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Bibliographic Details
Main Author: Yaacob, Nik Soriani
Format: Monograph
Language:English
Published: Pusat Pengajian Sains Perubatan 2013
Subjects:
Online Access:http://eprints.usm.my/54920/
http://eprints.usm.my/54920/1/NIK%20SORIANI%20YAACOB.pdf
Description
Summary:To quantify the mRNA expression levels of PPARy in the cervical cancer cell line, HeLa, using real-time PCR. To correlate the findings in (1) with the corresponding protein expression levels by Western blotting. To correlate the transcriptional and translational levels of PPARy with markers of apoptosis in these cells. To determine whether addition of specific PPARy ligands to the tumour cell line would modulate the above parameters.