Screening and characterization of 10398a polymorphism in invasive breast cancer in Malay population of peninsular Malaysia
The rnitochd~drial DNA (mtDNA) 10398 polymorphism is hypothesised to alter a mitochondrial subunit of the electron transfer chain and is associated with several neurodegenerative disorders and cancers. Methods: In this study, an mtDNApolymorphism at nucleotide position 10398 was screened in 101...
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| Format: | Monograph |
| Language: | English |
| Published: |
Pusat Pengajian Sains Kesihatan
2013
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| Online Access: | http://eprints.usm.my/54878/ http://eprints.usm.my/54878/1/MADYA%20DR.%20ZAFARINA%20ZAINUDDIN%20-%20e.pdf |
| _version_ | 1848882927493971968 |
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| author | Zafarina, Zainuddin |
| author_facet | Zafarina, Zainuddin |
| author_sort | Zafarina, Zainuddin |
| building | USM Institutional Repository |
| collection | Online Access |
| description | The rnitochd~drial DNA (mtDNA) 10398 polymorphism is hypothesised
to alter a mitochondrial subunit of the electron transfer chain and is associated with several
neurodegenerative disorders and cancers.
Methods: In this study, an mtDNApolymorphism at nucleotide position 10398 was screened
in 101 Malay female patients with invasive breast cancer and 90 age-matched healthy female controls
using minisequencing analysis.
Results: The Malay women with the 10398G variant showed a significantly increased risk of
invasive breast cancer (OR = 2.29, 95% CI 1.25-4.20, P = 0.007). lnmlunohistochemistry analysis
was conducted to investigate the effect of this polymorphism on the levels of apoptosis in breast
cancer cells. The level ofBax (a pro-apoptotic protein) expression was significantly higher than that
of Bcl-2 (an anti-apoptotic protein) in patients carrying the G allele (P = 0.016) but not in those
carrying the A allele (P = 0.48).
Conclusion: Based on these findings, we propose that the mtDNA 10398 polymorphism may
be a potential risk marker for breast cancer susceptibility in the Malay population. |
| first_indexed | 2025-11-15T18:42:41Z |
| format | Monograph |
| id | usm-54878 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T18:42:41Z |
| publishDate | 2013 |
| publisher | Pusat Pengajian Sains Kesihatan |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-548782022-09-22T06:56:04Z http://eprints.usm.my/54878/ Screening and characterization of 10398a polymorphism in invasive breast cancer in Malay population of peninsular Malaysia Zafarina, Zainuddin R Medicine (General) The rnitochd~drial DNA (mtDNA) 10398 polymorphism is hypothesised to alter a mitochondrial subunit of the electron transfer chain and is associated with several neurodegenerative disorders and cancers. Methods: In this study, an mtDNApolymorphism at nucleotide position 10398 was screened in 101 Malay female patients with invasive breast cancer and 90 age-matched healthy female controls using minisequencing analysis. Results: The Malay women with the 10398G variant showed a significantly increased risk of invasive breast cancer (OR = 2.29, 95% CI 1.25-4.20, P = 0.007). lnmlunohistochemistry analysis was conducted to investigate the effect of this polymorphism on the levels of apoptosis in breast cancer cells. The level ofBax (a pro-apoptotic protein) expression was significantly higher than that of Bcl-2 (an anti-apoptotic protein) in patients carrying the G allele (P = 0.016) but not in those carrying the A allele (P = 0.48). Conclusion: Based on these findings, we propose that the mtDNA 10398 polymorphism may be a potential risk marker for breast cancer susceptibility in the Malay population. Pusat Pengajian Sains Kesihatan 2013 Monograph NonPeerReviewed application/pdf en http://eprints.usm.my/54878/1/MADYA%20DR.%20ZAFARINA%20ZAINUDDIN%20-%20e.pdf Zafarina, Zainuddin (2013) Screening and characterization of 10398a polymorphism in invasive breast cancer in Malay population of peninsular Malaysia. Other. Pusat Pengajian Sains Kesihatan. (Submitted) |
| spellingShingle | R Medicine (General) Zafarina, Zainuddin Screening and characterization of 10398a polymorphism in invasive breast cancer in Malay population of peninsular Malaysia |
| title | Screening and characterization of 10398a
polymorphism in invasive breast cancer in
Malay population of peninsular Malaysia |
| title_full | Screening and characterization of 10398a
polymorphism in invasive breast cancer in
Malay population of peninsular Malaysia |
| title_fullStr | Screening and characterization of 10398a
polymorphism in invasive breast cancer in
Malay population of peninsular Malaysia |
| title_full_unstemmed | Screening and characterization of 10398a
polymorphism in invasive breast cancer in
Malay population of peninsular Malaysia |
| title_short | Screening and characterization of 10398a
polymorphism in invasive breast cancer in
Malay population of peninsular Malaysia |
| title_sort | screening and characterization of 10398a
polymorphism in invasive breast cancer in
malay population of peninsular malaysia |
| topic | R Medicine (General) |
| url | http://eprints.usm.my/54878/ http://eprints.usm.my/54878/1/MADYA%20DR.%20ZAFARINA%20ZAINUDDIN%20-%20e.pdf |