On The 2,6,6 – Trimethylcyclohex-2-Ene 1,4-Dione Biotransformation Beyond The Exponential Phase Of Baker’s Yeast Type-Ii Growth Curve
The reduction of ketoisophorone as a substrate has long become an interest for its valuable products as well as its interesting mechanism through biotransformation. A study of biotransformation of ketoisophorone beyond the exponential phase by non-growing cells has been conducted since there is la...
| Main Author: | |
|---|---|
| Format: | Monograph |
| Language: | English |
| Published: |
Universiti Sains Malaysia
2018
|
| Subjects: | |
| Online Access: | http://eprints.usm.my/53933/ http://eprints.usm.my/53933/1/On%20The%202%2C6%2C6%20%E2%80%93%20Trimethylcyclohex-2-Ene%201%2C4-Dione%20Biotransformation%20Beyond%20The%20Exponential%20Phase%20Of%20Baker%E2%80%99s%20Yeast%20Type-Ii%20Growth%20Curve_Siti%20Umiyah%20Othman_K4_2018.pdf |
| _version_ | 1848882664143060992 |
|---|---|
| author | Othman, Siti Umiyah |
| author_facet | Othman, Siti Umiyah |
| author_sort | Othman, Siti Umiyah |
| building | USM Institutional Repository |
| collection | Online Access |
| description | The reduction of ketoisophorone as a substrate has long become an interest for its valuable products as well as its interesting mechanism through biotransformation. A study
of biotransformation of ketoisophorone beyond the exponential phase by non-growing cells has been conducted since there is lack of number of research had been done about biotransformation of ketoisophorone during this phase. The main purpose of this research is to study the growth profile of Saccharomyces cerevisiae and to investigate the amount of
product which is actinol formed during stationary phase biotransformation. From the first objective, the growth profile was obtained and substrate introduction (ketoisophorone) was
deduced to be made at the 12th hour. Biotransformation was carried out in shake-flask by adding non-growing cells of S.cerevisiae into culture medium. A model of 7820 Gas
Chromatography was used to analyze the consumption of ketoisophorone, formation of (6R)-levodione as intermediate and (4R,6R)-actinol as the product. The results showed that
production of both (6R)-levodione and actinol was only produced in a small amount. It may be due to no or low activity of carbonyl reductase or the conversion of substrate to product
was too fast. For the product formation, actinol is strongly depends on the concentration of ketoisophorone. As the concentration of ketoisophorone is lower, the product formation
will be lower too. As the time passes, concentration of substrate is reduced as it was consumed by the cells, so the actinol formation was reduced too. |
| first_indexed | 2025-11-15T18:38:30Z |
| format | Monograph |
| id | usm-53933 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T18:38:30Z |
| publishDate | 2018 |
| publisher | Universiti Sains Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-539332022-08-09T08:45:04Z http://eprints.usm.my/53933/ On The 2,6,6 – Trimethylcyclohex-2-Ene 1,4-Dione Biotransformation Beyond The Exponential Phase Of Baker’s Yeast Type-Ii Growth Curve Othman, Siti Umiyah T Technology TP Chemical Technology The reduction of ketoisophorone as a substrate has long become an interest for its valuable products as well as its interesting mechanism through biotransformation. A study of biotransformation of ketoisophorone beyond the exponential phase by non-growing cells has been conducted since there is lack of number of research had been done about biotransformation of ketoisophorone during this phase. The main purpose of this research is to study the growth profile of Saccharomyces cerevisiae and to investigate the amount of product which is actinol formed during stationary phase biotransformation. From the first objective, the growth profile was obtained and substrate introduction (ketoisophorone) was deduced to be made at the 12th hour. Biotransformation was carried out in shake-flask by adding non-growing cells of S.cerevisiae into culture medium. A model of 7820 Gas Chromatography was used to analyze the consumption of ketoisophorone, formation of (6R)-levodione as intermediate and (4R,6R)-actinol as the product. The results showed that production of both (6R)-levodione and actinol was only produced in a small amount. It may be due to no or low activity of carbonyl reductase or the conversion of substrate to product was too fast. For the product formation, actinol is strongly depends on the concentration of ketoisophorone. As the concentration of ketoisophorone is lower, the product formation will be lower too. As the time passes, concentration of substrate is reduced as it was consumed by the cells, so the actinol formation was reduced too. Universiti Sains Malaysia 2018-06-01 Monograph NonPeerReviewed application/pdf en http://eprints.usm.my/53933/1/On%20The%202%2C6%2C6%20%E2%80%93%20Trimethylcyclohex-2-Ene%201%2C4-Dione%20Biotransformation%20Beyond%20The%20Exponential%20Phase%20Of%20Baker%E2%80%99s%20Yeast%20Type-Ii%20Growth%20Curve_Siti%20Umiyah%20Othman_K4_2018.pdf Othman, Siti Umiyah (2018) On The 2,6,6 – Trimethylcyclohex-2-Ene 1,4-Dione Biotransformation Beyond The Exponential Phase Of Baker’s Yeast Type-Ii Growth Curve. Project Report. Universiti Sains Malaysia, Pusat Pengajian Kejuruteraan Kimia. (Submitted) |
| spellingShingle | T Technology TP Chemical Technology Othman, Siti Umiyah On The 2,6,6 – Trimethylcyclohex-2-Ene 1,4-Dione Biotransformation Beyond The Exponential Phase Of Baker’s Yeast Type-Ii Growth Curve |
| title | On The 2,6,6 – Trimethylcyclohex-2-Ene 1,4-Dione Biotransformation Beyond The Exponential Phase Of Baker’s Yeast Type-Ii Growth Curve |
| title_full | On The 2,6,6 – Trimethylcyclohex-2-Ene 1,4-Dione Biotransformation Beyond The Exponential Phase Of Baker’s Yeast Type-Ii Growth Curve |
| title_fullStr | On The 2,6,6 – Trimethylcyclohex-2-Ene 1,4-Dione Biotransformation Beyond The Exponential Phase Of Baker’s Yeast Type-Ii Growth Curve |
| title_full_unstemmed | On The 2,6,6 – Trimethylcyclohex-2-Ene 1,4-Dione Biotransformation Beyond The Exponential Phase Of Baker’s Yeast Type-Ii Growth Curve |
| title_short | On The 2,6,6 – Trimethylcyclohex-2-Ene 1,4-Dione Biotransformation Beyond The Exponential Phase Of Baker’s Yeast Type-Ii Growth Curve |
| title_sort | on the 2,6,6 – trimethylcyclohex-2-ene 1,4-dione biotransformation beyond the exponential phase of baker’s yeast type-ii growth curve |
| topic | T Technology TP Chemical Technology |
| url | http://eprints.usm.my/53933/ http://eprints.usm.my/53933/1/On%20The%202%2C6%2C6%20%E2%80%93%20Trimethylcyclohex-2-Ene%201%2C4-Dione%20Biotransformation%20Beyond%20The%20Exponential%20Phase%20Of%20Baker%E2%80%99s%20Yeast%20Type-Ii%20Growth%20Curve_Siti%20Umiyah%20Othman_K4_2018.pdf |