On 2,6,6-Trimethylcyclohex-2-Ene-1,4-Dione Biotransformation Beyond The Stationary Phase Of Baker’s Yeast Type III
The reduction of ketoisophorone by the whole cell, Baker’s yeast cell type II has been researched recently. One of the major product of the biotransformation reduction of 2,6,6- trimethylcyclohex-2-ene-1,4-dione (ketoisophorone) is (4R,6R)-4-hydroxy-2,6,6-trimethylcyclohexanone or also named as (4R...
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| Format: | Monograph |
| Language: | English |
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Universiti Sains Malaysia
2018
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| Online Access: | http://eprints.usm.my/53930/ http://eprints.usm.my/53930/1/On%202%2C6%2C6-Trimethylcyclohex-2-Ene-1%2C4-Dione%20Biotransformation%20Beyond%20The%20Stationary%20Phase%20Of%20Baker%E2%80%99s%20Yeast%20Type%20III_Mohd%20Ridzwan%20Remlee_K4_2018.pdf |
| _version_ | 1848882663355580416 |
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| author | Remlee, Mohd Ridzwan |
| author_facet | Remlee, Mohd Ridzwan |
| author_sort | Remlee, Mohd Ridzwan |
| building | USM Institutional Repository |
| collection | Online Access |
| description | The reduction of ketoisophorone by the whole cell, Baker’s yeast cell type II has been researched recently. One of the major product of the biotransformation reduction of 2,6,6-
trimethylcyclohex-2-ene-1,4-dione (ketoisophorone) is (4R,6R)-4-hydroxy-2,6,6-trimethylcyclohexanone or also named as (4R,6R)-actinol using Saccharomyces cerevisiae in the present of glucose as the carbon source. The biotransformation of
ketoisophorone (KIP) forms levodione also named as 6R-dihydro-oxoisophorone (DOIP), which is intermediate before further reduction into actinol (ACT). This experiment only focussed on the death phase of S. cerevisiae fermentation where the substrate, ketoisophorone(KIP) was introduced and the time duration of 22h of death phase which is started from 38th hour until 60th of fermentation of yeast cells. The availability and
stability of cofactor, nicotinamide adenine dinucleotide (NADH) or nicotinamide adenine dinucleotide phosphate (NADPH) by the presence of glucose through Kreb’s cycle process was essential in the biotransformation of ketoisophorone during that phase was determined by the reading of absorbance using UV-Vis spectrophotometer. This work was also investigate the amount of substrate and intermediate present during 22 h of reaction in order to determine the activity of the two types of ezymes within the yeast cells which is responsible for this biotransformation which is carbonyl reductase (CBR) and enoate reductase (ER) to produce a chiral alcohol of (4R,6R)-actinol. The result showed that the death phase produced the intermediate as well as product which is actinol with the aid of cofactor availability during the reaction of biotransformation. |
| first_indexed | 2025-11-15T18:38:30Z |
| format | Monograph |
| id | usm-53930 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T18:38:30Z |
| publishDate | 2018 |
| publisher | Universiti Sains Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-539302022-08-09T08:35:57Z http://eprints.usm.my/53930/ On 2,6,6-Trimethylcyclohex-2-Ene-1,4-Dione Biotransformation Beyond The Stationary Phase Of Baker’s Yeast Type III Remlee, Mohd Ridzwan T Technology TP Chemical Technology The reduction of ketoisophorone by the whole cell, Baker’s yeast cell type II has been researched recently. One of the major product of the biotransformation reduction of 2,6,6- trimethylcyclohex-2-ene-1,4-dione (ketoisophorone) is (4R,6R)-4-hydroxy-2,6,6-trimethylcyclohexanone or also named as (4R,6R)-actinol using Saccharomyces cerevisiae in the present of glucose as the carbon source. The biotransformation of ketoisophorone (KIP) forms levodione also named as 6R-dihydro-oxoisophorone (DOIP), which is intermediate before further reduction into actinol (ACT). This experiment only focussed on the death phase of S. cerevisiae fermentation where the substrate, ketoisophorone(KIP) was introduced and the time duration of 22h of death phase which is started from 38th hour until 60th of fermentation of yeast cells. The availability and stability of cofactor, nicotinamide adenine dinucleotide (NADH) or nicotinamide adenine dinucleotide phosphate (NADPH) by the presence of glucose through Kreb’s cycle process was essential in the biotransformation of ketoisophorone during that phase was determined by the reading of absorbance using UV-Vis spectrophotometer. This work was also investigate the amount of substrate and intermediate present during 22 h of reaction in order to determine the activity of the two types of ezymes within the yeast cells which is responsible for this biotransformation which is carbonyl reductase (CBR) and enoate reductase (ER) to produce a chiral alcohol of (4R,6R)-actinol. The result showed that the death phase produced the intermediate as well as product which is actinol with the aid of cofactor availability during the reaction of biotransformation. Universiti Sains Malaysia 2018-06-01 Monograph NonPeerReviewed application/pdf en http://eprints.usm.my/53930/1/On%202%2C6%2C6-Trimethylcyclohex-2-Ene-1%2C4-Dione%20Biotransformation%20Beyond%20The%20Stationary%20Phase%20Of%20Baker%E2%80%99s%20Yeast%20Type%20III_Mohd%20Ridzwan%20Remlee_K4_2018.pdf Remlee, Mohd Ridzwan (2018) On 2,6,6-Trimethylcyclohex-2-Ene-1,4-Dione Biotransformation Beyond The Stationary Phase Of Baker’s Yeast Type III. Project Report. Universiti Sains Malaysia, Pusat Pengajian Kejuruteraan Kimia. (Submitted) |
| spellingShingle | T Technology TP Chemical Technology Remlee, Mohd Ridzwan On 2,6,6-Trimethylcyclohex-2-Ene-1,4-Dione Biotransformation Beyond The Stationary Phase Of Baker’s Yeast Type III |
| title | On 2,6,6-Trimethylcyclohex-2-Ene-1,4-Dione Biotransformation Beyond The Stationary Phase Of Baker’s Yeast Type III |
| title_full | On 2,6,6-Trimethylcyclohex-2-Ene-1,4-Dione Biotransformation Beyond The Stationary Phase Of Baker’s Yeast Type III |
| title_fullStr | On 2,6,6-Trimethylcyclohex-2-Ene-1,4-Dione Biotransformation Beyond The Stationary Phase Of Baker’s Yeast Type III |
| title_full_unstemmed | On 2,6,6-Trimethylcyclohex-2-Ene-1,4-Dione Biotransformation Beyond The Stationary Phase Of Baker’s Yeast Type III |
| title_short | On 2,6,6-Trimethylcyclohex-2-Ene-1,4-Dione Biotransformation Beyond The Stationary Phase Of Baker’s Yeast Type III |
| title_sort | on 2,6,6-trimethylcyclohex-2-ene-1,4-dione biotransformation beyond the stationary phase of baker’s yeast type iii |
| topic | T Technology TP Chemical Technology |
| url | http://eprints.usm.my/53930/ http://eprints.usm.my/53930/1/On%202%2C6%2C6-Trimethylcyclohex-2-Ene-1%2C4-Dione%20Biotransformation%20Beyond%20The%20Stationary%20Phase%20Of%20Baker%E2%80%99s%20Yeast%20Type%20III_Mohd%20Ridzwan%20Remlee_K4_2018.pdf |