CB1 cannabinoid receptor in brain mesolimbic system of mitragynine-sensitised albina wastar rats as a candidate molecular terget in mitragyna speciosa (KETUM) addiction
Emerging new psychoactive substances impose public health implications worldwide, and include plants' phytochemicals such as Mitragynine (MG) from Mitragyna speciosa (or 'Ketum'). Studies have demonstrated the reciprocal interaction between endocannabinoid (ECB) system and opioid sy...
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| Format: | Monograph |
| Language: | English |
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Pusat Pengajian Sains Perubatan, Universiti Sains Malaysia
2015
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| Online Access: | http://eprints.usm.my/53465/ http://eprints.usm.my/53465/1/DR.%20MUZAIMI%20MUSTAPHA-Eprints.pdf |
| _version_ | 1848882536144437248 |
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| author | Mustapha, Muzaimi |
| author_facet | Mustapha, Muzaimi |
| author_sort | Mustapha, Muzaimi |
| building | USM Institutional Repository |
| collection | Online Access |
| description | Emerging new psychoactive substances impose public health implications worldwide, and include plants'
phytochemicals such as Mitragynine (MG) from Mitragyna speciosa (or 'Ketum'). Studies have demonstrated
the reciprocal interaction between endocannabinoid (ECB) system and opioid systems in the modulation of
brain-reward and neurobiological mechanisms underlying drug addiction. To date the neurochemical basis of
Ketum abuse potential remains elusive. This research attempted to implicate ECB system in the abuse
liabilities of emerging psychoactive plant, Ketum and its opioid-like alkaloid, mitragynine (MG). We had
demonstrated the locomotor and behavioural effects of MG-sensitised Swiss albino mice in lntelliCage® using
an approach of context-independent sensitisation. Animals were later sacrificed for immunohistochemical
localisation of CB1 receptor in the brain with confirmatory analysis using Western blotting of the brain
homogenates. Adult Albino Swiss mice were subjected to experimental groups of MG alone; MG + NIDA-
41020 (CB1 receptor antagonist); Morphine sulphate; .0.-9-Tetrahydrocannabinol + NIDA-41020; and vehicle.
Daily intraperitoneal injection (i.p) of MG (5 to 25 mg/kg; 5mg/kg increment) at a 6-day interval was
administered for 30 days, and plus once daily, 20mg NIDA-41 020 (oral). Findings to date showed
neuroadaptive chronic exposure of morphine, and works are in its final phase for comparison with MG and in
the presence of CB1 antagonist. Data analysis to date provide supportive ground for the first time to our
knowledge, to indicate the involvement of CB1 receptor as the neural target of Ketum misuse potential. (Two
manuscripts in preparation for potential publication in indexed-journals). |
| first_indexed | 2025-11-15T18:36:28Z |
| format | Monograph |
| id | usm-53465 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T18:36:28Z |
| publishDate | 2015 |
| publisher | Pusat Pengajian Sains Perubatan, Universiti Sains Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-534652022-07-20T01:48:36Z http://eprints.usm.my/53465/ CB1 cannabinoid receptor in brain mesolimbic system of mitragynine-sensitised albina wastar rats as a candidate molecular terget in mitragyna speciosa (KETUM) addiction Mustapha, Muzaimi R Medicine Emerging new psychoactive substances impose public health implications worldwide, and include plants' phytochemicals such as Mitragynine (MG) from Mitragyna speciosa (or 'Ketum'). Studies have demonstrated the reciprocal interaction between endocannabinoid (ECB) system and opioid systems in the modulation of brain-reward and neurobiological mechanisms underlying drug addiction. To date the neurochemical basis of Ketum abuse potential remains elusive. This research attempted to implicate ECB system in the abuse liabilities of emerging psychoactive plant, Ketum and its opioid-like alkaloid, mitragynine (MG). We had demonstrated the locomotor and behavioural effects of MG-sensitised Swiss albino mice in lntelliCage® using an approach of context-independent sensitisation. Animals were later sacrificed for immunohistochemical localisation of CB1 receptor in the brain with confirmatory analysis using Western blotting of the brain homogenates. Adult Albino Swiss mice were subjected to experimental groups of MG alone; MG + NIDA- 41020 (CB1 receptor antagonist); Morphine sulphate; .0.-9-Tetrahydrocannabinol + NIDA-41020; and vehicle. Daily intraperitoneal injection (i.p) of MG (5 to 25 mg/kg; 5mg/kg increment) at a 6-day interval was administered for 30 days, and plus once daily, 20mg NIDA-41 020 (oral). Findings to date showed neuroadaptive chronic exposure of morphine, and works are in its final phase for comparison with MG and in the presence of CB1 antagonist. Data analysis to date provide supportive ground for the first time to our knowledge, to indicate the involvement of CB1 receptor as the neural target of Ketum misuse potential. (Two manuscripts in preparation for potential publication in indexed-journals). Pusat Pengajian Sains Perubatan, Universiti Sains Malaysia 2015 Monograph NonPeerReviewed application/pdf en http://eprints.usm.my/53465/1/DR.%20MUZAIMI%20MUSTAPHA-Eprints.pdf Mustapha, Muzaimi (2015) CB1 cannabinoid receptor in brain mesolimbic system of mitragynine-sensitised albina wastar rats as a candidate molecular terget in mitragyna speciosa (KETUM) addiction. Other. Pusat Pengajian Sains Perubatan, Universiti Sains Malaysia. (Submitted) |
| spellingShingle | R Medicine Mustapha, Muzaimi CB1 cannabinoid receptor in brain mesolimbic system of mitragynine-sensitised albina wastar rats as a candidate molecular terget in mitragyna speciosa (KETUM) addiction |
| title | CB1 cannabinoid receptor in brain mesolimbic
system of mitragynine-sensitised albina wastar
rats as a candidate molecular terget in
mitragyna speciosa (KETUM) addiction |
| title_full | CB1 cannabinoid receptor in brain mesolimbic
system of mitragynine-sensitised albina wastar
rats as a candidate molecular terget in
mitragyna speciosa (KETUM) addiction |
| title_fullStr | CB1 cannabinoid receptor in brain mesolimbic
system of mitragynine-sensitised albina wastar
rats as a candidate molecular terget in
mitragyna speciosa (KETUM) addiction |
| title_full_unstemmed | CB1 cannabinoid receptor in brain mesolimbic
system of mitragynine-sensitised albina wastar
rats as a candidate molecular terget in
mitragyna speciosa (KETUM) addiction |
| title_short | CB1 cannabinoid receptor in brain mesolimbic
system of mitragynine-sensitised albina wastar
rats as a candidate molecular terget in
mitragyna speciosa (KETUM) addiction |
| title_sort | cb1 cannabinoid receptor in brain mesolimbic
system of mitragynine-sensitised albina wastar
rats as a candidate molecular terget in
mitragyna speciosa (ketum) addiction |
| topic | R Medicine |
| url | http://eprints.usm.my/53465/ http://eprints.usm.my/53465/1/DR.%20MUZAIMI%20MUSTAPHA-Eprints.pdf |