Expression Of PhaC1 And PhaC2 From Pseudomonas Sp. USM4-55 In Escherichia Coli Expression System
Polyhydroxyalkanoates (PHAs) are synthesized intracelullarly by a variety of microorganisms as reserve materials for carbon and energy source. PHA synthase (PhaC) is the key enzyme in the polymerization of PHAs. Pseudomonas sp. USM4-55 is a local isolate which is able to produce a blend of short...
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| Format: | Thesis |
| Language: | English |
| Published: |
2007
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| Online Access: | http://eprints.usm.my/53174/ http://eprints.usm.my/53174/1/Tesis%20expression%20of%20phaC1%20cut.pdf |
| _version_ | 1848882454043033600 |
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| author | Ghani, Balqis Ab. |
| author_facet | Ghani, Balqis Ab. |
| author_sort | Ghani, Balqis Ab. |
| building | USM Institutional Repository |
| collection | Online Access |
| description | Polyhydroxyalkanoates (PHAs) are synthesized intracelullarly by a variety of microorganisms as reserve materials for carbon and energy source.
PHA synthase (PhaC) is the key enzyme in the polymerization of PHAs.
Pseudomonas sp. USM4-55 is a local isolate which is able to produce a blend
of short chain length and medium chain length of polymer. Genes coding for
PhaC (phaC1 and phaC2) from Pseudomonas sp. USM4-55 were amplified
(each with a size of 1.7 kb) from the genomic DNA, cloned into pGEMR-T for
sequencing and then expressed. For expression studies, pQE-30 was used as
the expression vector under the control of PT5 promoter. These clones were
named pQE-C1 and pQE-C2, and were induced with 0.01 mM of isopropyl-p-Dthiogalactoside
(IPTG) at ODeoo of 0.6. |
| first_indexed | 2025-11-15T18:35:10Z |
| format | Thesis |
| id | usm-53174 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T18:35:10Z |
| publishDate | 2007 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-531742022-06-29T02:52:05Z http://eprints.usm.my/53174/ Expression Of PhaC1 And PhaC2 From Pseudomonas Sp. USM4-55 In Escherichia Coli Expression System Ghani, Balqis Ab. QH1 Natural history (General - Including nature conservation, geographical distribution) Polyhydroxyalkanoates (PHAs) are synthesized intracelullarly by a variety of microorganisms as reserve materials for carbon and energy source. PHA synthase (PhaC) is the key enzyme in the polymerization of PHAs. Pseudomonas sp. USM4-55 is a local isolate which is able to produce a blend of short chain length and medium chain length of polymer. Genes coding for PhaC (phaC1 and phaC2) from Pseudomonas sp. USM4-55 were amplified (each with a size of 1.7 kb) from the genomic DNA, cloned into pGEMR-T for sequencing and then expressed. For expression studies, pQE-30 was used as the expression vector under the control of PT5 promoter. These clones were named pQE-C1 and pQE-C2, and were induced with 0.01 mM of isopropyl-p-Dthiogalactoside (IPTG) at ODeoo of 0.6. 2007-09 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/53174/1/Tesis%20expression%20of%20phaC1%20cut.pdf Ghani, Balqis Ab. (2007) Expression Of PhaC1 And PhaC2 From Pseudomonas Sp. USM4-55 In Escherichia Coli Expression System. Masters thesis, Universiti Sains Malaysia. |
| spellingShingle | QH1 Natural history (General - Including nature conservation, geographical distribution) Ghani, Balqis Ab. Expression Of PhaC1 And PhaC2 From Pseudomonas Sp. USM4-55 In Escherichia Coli Expression System |
| title | Expression Of PhaC1 And PhaC2 From
Pseudomonas Sp. USM4-55 In
Escherichia Coli Expression System |
| title_full | Expression Of PhaC1 And PhaC2 From
Pseudomonas Sp. USM4-55 In
Escherichia Coli Expression System |
| title_fullStr | Expression Of PhaC1 And PhaC2 From
Pseudomonas Sp. USM4-55 In
Escherichia Coli Expression System |
| title_full_unstemmed | Expression Of PhaC1 And PhaC2 From
Pseudomonas Sp. USM4-55 In
Escherichia Coli Expression System |
| title_short | Expression Of PhaC1 And PhaC2 From
Pseudomonas Sp. USM4-55 In
Escherichia Coli Expression System |
| title_sort | expression of phac1 and phac2 from
pseudomonas sp. usm4-55 in
escherichia coli expression system |
| topic | QH1 Natural history (General - Including nature conservation, geographical distribution) |
| url | http://eprints.usm.my/53174/ http://eprints.usm.my/53174/1/Tesis%20expression%20of%20phaC1%20cut.pdf |