Biosynthesis Of Silver Nanoparticles Using Plant Extract Of Colubrina Asiatica And Its Characterization
Biosynthesis of silver nanoparticles (AgNPs) using plant extract (PE) has gained great interest recently, mainly due to their tremendous availability and various arrays of reducing metabolites. In this study, we investigated the reduction ability of aqueous extract of Colubrina asiatica in order to...
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| Format: | Monograph |
| Language: | English |
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Universiti Sains Malaysia
2017
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| Online Access: | http://eprints.usm.my/53137/ http://eprints.usm.my/53137/1/Biosynthesis%20Of%20Silver%20Nanoparticles%20Using%20Plant%20Extract%20Of%20Colubrina%20Asiatica%20And%20Its%20Characterization_Nor%20Ezaney%20Ibrahim_K4_2017.pdf |
| Summary: | Biosynthesis of silver nanoparticles (AgNPs) using plant extract (PE) has gained great interest recently, mainly due to their tremendous availability and various arrays of reducing metabolites. In this study, we investigated the reduction ability of aqueous extract of Colubrina asiatica in order to synthesize AgNPs. Optimal synthesis of AgNPs was investigated using UV-Visible Spectroscopy, Transmission Electron Microscopy (TEM), Fourier Transform Infrared (FTIR) and Dynamic Light Scattering (DLS). Different parameters which include concentration of PE solution, concentration of silver nitrate (AgNO3), ratio of PE to the AgNO3 solution and incubation period that affect the synthesis of AgNPs was investigated. Colubrina asiatica can reduces silver ions to silver nanoparticles within 24 hr as indicated by the color changes from pale yellow to yellowish brown. The UV-Vis spectrum of AgNPs revealed a characteristic Surface Plasmon Resonance (SPR) peak at 445 nm. Transmission electron microscope (TEM) confirmed the spherical nature and the crystallinity of nanoparticles. Fourier Transform Infrared (FTIR) spectra prove that various functional groups of biomolecules capping the nanoparticles. The average size of nanoparticles was 97 nm as determined by DLS. This study presents a comprehensive investigation of the differential behavioural of PE and AgNO3 to synthesize biologically stable AgNPs. |
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