Development Of Multiplex Real-Time Reverse Transcription Pcr (qRT-PCR) Assay For Detection And Subtyping Of Avian Influenza Virus
Avian flu is a serious disease that always give rise to devastated consequences in poultry industry. This disease is caused by influenza A virus bird’s strain. The genome of avian strain of influenza A virus (AIV) exhibits high mutation rate by means of antigenic drift and shift, leading to the emer...
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| Format: | Thesis |
| Language: | English |
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2020
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| Online Access: | http://eprints.usm.my/52088/ http://eprints.usm.my/52088/1/Pages%20from%20Development%20Of%20Multiplex%20Real-Time%20Reverse%20Transcription%20Pcr%20%28qRT-PCR%29.pdf |
| _version_ | 1848882162759106560 |
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| author | Yee, May Ye |
| author_facet | Yee, May Ye |
| author_sort | Yee, May Ye |
| building | USM Institutional Repository |
| collection | Online Access |
| description | Avian flu is a serious disease that always give rise to devastated consequences in poultry industry. This disease is caused by influenza A virus bird’s strain. The genome of avian strain of influenza A virus (AIV) exhibits high mutation rate by means of antigenic drift and shift, leading to the emergence of historic pandemics and epidemics. The first line of defence against avian influenza relies on rapid diagnosis. Current singleplex real-time RT-PCR (qRT-PCR) assay is not cost effective and internal control is not included. In this study, an in-house, two-stage multiplex qRT-PCR assay was developed for simultaneous detection and subtyping of H5, H7 and H9 subtype of AIV, the three most common subtypes isolated from poultry. |
| first_indexed | 2025-11-15T18:30:32Z |
| format | Thesis |
| id | usm-52088 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T18:30:32Z |
| publishDate | 2020 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-520882022-05-26T04:48:05Z http://eprints.usm.my/52088/ Development Of Multiplex Real-Time Reverse Transcription Pcr (qRT-PCR) Assay For Detection And Subtyping Of Avian Influenza Virus Yee, May Ye R735-854 Medical education. Medical schools. Research Avian flu is a serious disease that always give rise to devastated consequences in poultry industry. This disease is caused by influenza A virus bird’s strain. The genome of avian strain of influenza A virus (AIV) exhibits high mutation rate by means of antigenic drift and shift, leading to the emergence of historic pandemics and epidemics. The first line of defence against avian influenza relies on rapid diagnosis. Current singleplex real-time RT-PCR (qRT-PCR) assay is not cost effective and internal control is not included. In this study, an in-house, two-stage multiplex qRT-PCR assay was developed for simultaneous detection and subtyping of H5, H7 and H9 subtype of AIV, the three most common subtypes isolated from poultry. 2020-11 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/52088/1/Pages%20from%20Development%20Of%20Multiplex%20Real-Time%20Reverse%20Transcription%20Pcr%20%28qRT-PCR%29.pdf Yee, May Ye (2020) Development Of Multiplex Real-Time Reverse Transcription Pcr (qRT-PCR) Assay For Detection And Subtyping Of Avian Influenza Virus. Masters thesis, Universiti Sains Malaysia. |
| spellingShingle | R735-854 Medical education. Medical schools. Research Yee, May Ye Development Of Multiplex Real-Time Reverse Transcription Pcr (qRT-PCR) Assay For Detection And Subtyping Of Avian Influenza Virus |
| title | Development Of Multiplex Real-Time Reverse Transcription Pcr (qRT-PCR) Assay For Detection And Subtyping Of Avian Influenza Virus |
| title_full | Development Of Multiplex Real-Time Reverse Transcription Pcr (qRT-PCR) Assay For Detection And Subtyping Of Avian Influenza Virus |
| title_fullStr | Development Of Multiplex Real-Time Reverse Transcription Pcr (qRT-PCR) Assay For Detection And Subtyping Of Avian Influenza Virus |
| title_full_unstemmed | Development Of Multiplex Real-Time Reverse Transcription Pcr (qRT-PCR) Assay For Detection And Subtyping Of Avian Influenza Virus |
| title_short | Development Of Multiplex Real-Time Reverse Transcription Pcr (qRT-PCR) Assay For Detection And Subtyping Of Avian Influenza Virus |
| title_sort | development of multiplex real-time reverse transcription pcr (qrt-pcr) assay for detection and subtyping of avian influenza virus |
| topic | R735-854 Medical education. Medical schools. Research |
| url | http://eprints.usm.my/52088/ http://eprints.usm.my/52088/1/Pages%20from%20Development%20Of%20Multiplex%20Real-Time%20Reverse%20Transcription%20Pcr%20%28qRT-PCR%29.pdf |