Understanding the role of interleukin-17 A in bone remodelling mechanism of stem cells of human exfoliated deciduous teeth-derived osteoblast cells
Interleukin-HA (IL-17A) enhances the osteogenic differentiation and increases osteoprotegerin (OPG)/receptor activator of NF-KB ligand (RANKL) expression of the stem cells. from human deciduous teeth (SHED). The OPG/RANKL system is essential in bone metabolism where RANKL augments bone resorption...
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| Format: | Article |
| Language: | English |
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Pusat Pengajian Sains Kesihatan, Universiti Sains Malaysia
2019
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| Online Access: | http://eprints.usm.my/51677/ http://eprints.usm.my/51677/1/DR.%20NURUL%20ASMA%20ABDULLAH-Eprints.pdf |
| _version_ | 1848882051042770944 |
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| author | Abdullah, Nurul Asma |
| author_facet | Abdullah, Nurul Asma |
| author_sort | Abdullah, Nurul Asma |
| building | USM Institutional Repository |
| collection | Online Access |
| description | Interleukin-HA (IL-17A) enhances the osteogenic differentiation and increases osteoprotegerin
(OPG)/receptor activator of NF-KB ligand (RANKL) expression of the stem cells. from human
deciduous teeth (SHED). The OPG/RANKL system is essential in bone metabolism where
RANKL augments bone resorption whereas OPG favours osteogenesis. This study investigates
the effects of IL-17A on the osteogenic differentiation and its effect on the RANKUOPG
signalling pathway. IL-17A enhanced proliferation and alkaline phosphatase activity in SHED in
a dose-dependent manner. IL-17A also increased calcium deposition which indicated
mineralization activity. Moreover, the expressions of ALP, COL 1A1, RUNX2, OCN, OPN and
OPG were significantly up-regulated when treated with IL-17A In contrast, IL-17A downregulated
RANKL expression in SHED. Interestingly, the OPG/RANKL ratio was significantly
higher in IL-17A-treated groups. We also demonstrated that IL-17A activated MAPK signalling
pathway by significant up-regulation of upstream activators and downstream targets of ERK,
P38 and JNK pathway. We conclude that IL-17A demonstrates osteo-stimulating effects; thus
suggests the use of IL-17A in bone substitutes to promote bone regeneration. |
| first_indexed | 2025-11-15T18:28:46Z |
| format | Article |
| id | usm-51677 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T18:28:46Z |
| publishDate | 2019 |
| publisher | Pusat Pengajian Sains Kesihatan, Universiti Sains Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-516772022-02-27T04:05:42Z http://eprints.usm.my/51677/ Understanding the role of interleukin-17 A in bone remodelling mechanism of stem cells of human exfoliated deciduous teeth-derived osteoblast cells Abdullah, Nurul Asma R Medicine Interleukin-HA (IL-17A) enhances the osteogenic differentiation and increases osteoprotegerin (OPG)/receptor activator of NF-KB ligand (RANKL) expression of the stem cells. from human deciduous teeth (SHED). The OPG/RANKL system is essential in bone metabolism where RANKL augments bone resorption whereas OPG favours osteogenesis. This study investigates the effects of IL-17A on the osteogenic differentiation and its effect on the RANKUOPG signalling pathway. IL-17A enhanced proliferation and alkaline phosphatase activity in SHED in a dose-dependent manner. IL-17A also increased calcium deposition which indicated mineralization activity. Moreover, the expressions of ALP, COL 1A1, RUNX2, OCN, OPN and OPG were significantly up-regulated when treated with IL-17A In contrast, IL-17A downregulated RANKL expression in SHED. Interestingly, the OPG/RANKL ratio was significantly higher in IL-17A-treated groups. We also demonstrated that IL-17A activated MAPK signalling pathway by significant up-regulation of upstream activators and downstream targets of ERK, P38 and JNK pathway. We conclude that IL-17A demonstrates osteo-stimulating effects; thus suggests the use of IL-17A in bone substitutes to promote bone regeneration. Pusat Pengajian Sains Kesihatan, Universiti Sains Malaysia 2019 Article NonPeerReviewed application/pdf en http://eprints.usm.my/51677/1/DR.%20NURUL%20ASMA%20ABDULLAH-Eprints.pdf Abdullah, Nurul Asma (2019) Understanding the role of interleukin-17 A in bone remodelling mechanism of stem cells of human exfoliated deciduous teeth-derived osteoblast cells. Understanding the role of interleukin-17 A in bone remodelling mechanism of stem cells of human exfoliated deciduous teeth-derived osteoblast cells. |
| spellingShingle | R Medicine Abdullah, Nurul Asma Understanding the role of interleukin-17 A in bone remodelling mechanism of stem cells of human exfoliated deciduous teeth-derived osteoblast cells |
| title | Understanding the role of interleukin-17 A in
bone remodelling mechanism of stem cells of human
exfoliated deciduous teeth-derived osteoblast cells |
| title_full | Understanding the role of interleukin-17 A in
bone remodelling mechanism of stem cells of human
exfoliated deciduous teeth-derived osteoblast cells |
| title_fullStr | Understanding the role of interleukin-17 A in
bone remodelling mechanism of stem cells of human
exfoliated deciduous teeth-derived osteoblast cells |
| title_full_unstemmed | Understanding the role of interleukin-17 A in
bone remodelling mechanism of stem cells of human
exfoliated deciduous teeth-derived osteoblast cells |
| title_short | Understanding the role of interleukin-17 A in
bone remodelling mechanism of stem cells of human
exfoliated deciduous teeth-derived osteoblast cells |
| title_sort | understanding the role of interleukin-17 a in
bone remodelling mechanism of stem cells of human
exfoliated deciduous teeth-derived osteoblast cells |
| topic | R Medicine |
| url | http://eprints.usm.my/51677/ http://eprints.usm.my/51677/1/DR.%20NURUL%20ASMA%20ABDULLAH-Eprints.pdf |