Surface Functionalization And Characterization Of Antimicrobial Porous P(3hb-Co-4hb) Scaffold With Collagen Peptide For Biomedical Applications
P(3-hydroxybutyrate-co-4-hydroxybutyrate), [P(3HB-co-4HB)] is a bacterial derived biopolymer known for its high levels of biocompatability. However, surface of P(3HB-co-4HB) is found to be hydrophobic with minimal recognition sites for cell attachment. This makes it less desirable candidate to be ta...
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| Format: | Thesis |
| Language: | English |
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2020
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| Online Access: | http://eprints.usm.my/51671/ http://eprints.usm.my/51671/1/TANA%20POORANI%20AP%20GURUSAMY%20-%20TESIS%20cut.pdf |
| _version_ | 1848882049390215168 |
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| author | Gurusamy, Tana Poorani |
| author_facet | Gurusamy, Tana Poorani |
| author_sort | Gurusamy, Tana Poorani |
| building | USM Institutional Repository |
| collection | Online Access |
| description | P(3-hydroxybutyrate-co-4-hydroxybutyrate), [P(3HB-co-4HB)] is a bacterial derived biopolymer known for its high levels of biocompatability. However, surface of P(3HB-co-4HB) is found to be hydrophobic with minimal recognition sites for cell attachment. This makes it less desirable candidate to be tailored as scaffolds for tissue engineering and biomedical applications. Due to this, this research was undertaken to fabricate P(3HB-co-4HB) with collagen peptides scaffolds using physiochemical modifications to further enhance their surface wettabilty as well as to support cell growth for biomedical applications. In this case, a bacterial strain, Cupriavidus malaysienesis sp. USMAA 1020 transformant, was found to synthesize P(3HB-co-4HB) from 4-hydroxybutyrate (4HB) precursor substrates, namely 1,4-butanediol and 1,6-hexanediol, respectatively. Firstly, a porous P(3HB-co-4HB) was fabricated through salt leaching and freeze drying techniques. This was then followed by the incorporation of collagen peptides at various concentrations (2.5 wt% - 12.5 wt%) to P(3HB-co-4HB) using two different modifications technique, amiolysis and collagen-coating. As a result, three types of P(3HB-co-4HB) scaffolds was fabricated including silver sulfadiazine(SSD) coated/collagen peptide-coated P(3HB-co-4HB) scaffolds, silver sulfadiazine blend/collagen peptide-coated P(3HB-co-4HB) scaffold and silver sulfadiazine blend/aminolysed P(3HB-co-4HB). |
| first_indexed | 2025-11-15T18:28:44Z |
| format | Thesis |
| id | usm-51671 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T18:28:44Z |
| publishDate | 2020 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-516712022-02-25T08:56:38Z http://eprints.usm.my/51671/ Surface Functionalization And Characterization Of Antimicrobial Porous P(3hb-Co-4hb) Scaffold With Collagen Peptide For Biomedical Applications Gurusamy, Tana Poorani QH1-278.5 Natural history (General) P(3-hydroxybutyrate-co-4-hydroxybutyrate), [P(3HB-co-4HB)] is a bacterial derived biopolymer known for its high levels of biocompatability. However, surface of P(3HB-co-4HB) is found to be hydrophobic with minimal recognition sites for cell attachment. This makes it less desirable candidate to be tailored as scaffolds for tissue engineering and biomedical applications. Due to this, this research was undertaken to fabricate P(3HB-co-4HB) with collagen peptides scaffolds using physiochemical modifications to further enhance their surface wettabilty as well as to support cell growth for biomedical applications. In this case, a bacterial strain, Cupriavidus malaysienesis sp. USMAA 1020 transformant, was found to synthesize P(3HB-co-4HB) from 4-hydroxybutyrate (4HB) precursor substrates, namely 1,4-butanediol and 1,6-hexanediol, respectatively. Firstly, a porous P(3HB-co-4HB) was fabricated through salt leaching and freeze drying techniques. This was then followed by the incorporation of collagen peptides at various concentrations (2.5 wt% - 12.5 wt%) to P(3HB-co-4HB) using two different modifications technique, amiolysis and collagen-coating. As a result, three types of P(3HB-co-4HB) scaffolds was fabricated including silver sulfadiazine(SSD) coated/collagen peptide-coated P(3HB-co-4HB) scaffolds, silver sulfadiazine blend/collagen peptide-coated P(3HB-co-4HB) scaffold and silver sulfadiazine blend/aminolysed P(3HB-co-4HB). 2020-05 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/51671/1/TANA%20POORANI%20AP%20GURUSAMY%20-%20TESIS%20cut.pdf Gurusamy, Tana Poorani (2020) Surface Functionalization And Characterization Of Antimicrobial Porous P(3hb-Co-4hb) Scaffold With Collagen Peptide For Biomedical Applications. Masters thesis, Universiti Sains Malaysia. |
| spellingShingle | QH1-278.5 Natural history (General) Gurusamy, Tana Poorani Surface Functionalization And Characterization Of Antimicrobial Porous P(3hb-Co-4hb) Scaffold With Collagen Peptide For Biomedical Applications |
| title | Surface Functionalization And Characterization Of Antimicrobial Porous P(3hb-Co-4hb) Scaffold With Collagen Peptide For Biomedical Applications |
| title_full | Surface Functionalization And Characterization Of Antimicrobial Porous P(3hb-Co-4hb) Scaffold With Collagen Peptide For Biomedical Applications |
| title_fullStr | Surface Functionalization And Characterization Of Antimicrobial Porous P(3hb-Co-4hb) Scaffold With Collagen Peptide For Biomedical Applications |
| title_full_unstemmed | Surface Functionalization And Characterization Of Antimicrobial Porous P(3hb-Co-4hb) Scaffold With Collagen Peptide For Biomedical Applications |
| title_short | Surface Functionalization And Characterization Of Antimicrobial Porous P(3hb-Co-4hb) Scaffold With Collagen Peptide For Biomedical Applications |
| title_sort | surface functionalization and characterization of antimicrobial porous p(3hb-co-4hb) scaffold with collagen peptide for biomedical applications |
| topic | QH1-278.5 Natural history (General) |
| url | http://eprints.usm.my/51671/ http://eprints.usm.my/51671/1/TANA%20POORANI%20AP%20GURUSAMY%20-%20TESIS%20cut.pdf |