Elucidating the roles of annexin-like molecules in the protozoan parasite giardia intestinalis: an approach to the control of giardiasis

Giardiasis is a disease caused by protozoan parasite Giardia intestinalis. Herein, we sought to examine the role of alpha-giardins in response to hydrogen peroxide (H202) treatment in G. intestinalis. Results showed that Giard1a treated with increased concentration of H20 2 demonstrated significa...

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Main Author: Candy, Chuah
Format: Article
Language:English
Published: Pusat Pengajian Sains Perubatan, Universiti Sains Malaysia 2019
Subjects:
Online Access:http://eprints.usm.my/51603/
http://eprints.usm.my/51603/1/DR.%20CHUAH%20CANDY-Eprints.pdf
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author Candy, Chuah
author_facet Candy, Chuah
author_sort Candy, Chuah
building USM Institutional Repository
collection Online Access
description Giardiasis is a disease caused by protozoan parasite Giardia intestinalis. Herein, we sought to examine the role of alpha-giardins in response to hydrogen peroxide (H202) treatment in G. intestinalis. Results showed that Giard1a treated with increased concentration of H20 2 demonstrated significant reduction in cell viability and significant increase reactive oxygen species production. We found that alpha-2 and alpha-3 giardin showed significant up-regulated transcriptional expression in H,O,-treated Giardia at 1 h time-point, whereas gene expression level of alpha-7.2 and alpha-7.3 giardin up-regulated significantly at 4h time-point Alpha-11 giardin demonstrated up-regulated gene expression at a later time-point (8h and 24h) in H,02- treated Giardia. Together these findings suggest that alpha giardins could serve as potential promising target for future drug design and vaccine development
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spelling usm-516032022-02-22T01:52:49Z http://eprints.usm.my/51603/ Elucidating the roles of annexin-like molecules in the protozoan parasite giardia intestinalis: an approach to the control of giardiasis Candy, Chuah R Medicine Giardiasis is a disease caused by protozoan parasite Giardia intestinalis. Herein, we sought to examine the role of alpha-giardins in response to hydrogen peroxide (H202) treatment in G. intestinalis. Results showed that Giard1a treated with increased concentration of H20 2 demonstrated significant reduction in cell viability and significant increase reactive oxygen species production. We found that alpha-2 and alpha-3 giardin showed significant up-regulated transcriptional expression in H,O,-treated Giardia at 1 h time-point, whereas gene expression level of alpha-7.2 and alpha-7.3 giardin up-regulated significantly at 4h time-point Alpha-11 giardin demonstrated up-regulated gene expression at a later time-point (8h and 24h) in H,02- treated Giardia. Together these findings suggest that alpha giardins could serve as potential promising target for future drug design and vaccine development Pusat Pengajian Sains Perubatan, Universiti Sains Malaysia 2019 Article NonPeerReviewed application/pdf en http://eprints.usm.my/51603/1/DR.%20CHUAH%20CANDY-Eprints.pdf Candy, Chuah (2019) Elucidating the roles of annexin-like molecules in the protozoan parasite giardia intestinalis: an approach to the control of giardiasis. Elucidating the roles of annexin-like molecules in the protozoan parasite giardia intestinalis: an approach to the control of giardiasis.
spellingShingle R Medicine
Candy, Chuah
Elucidating the roles of annexin-like molecules in the protozoan parasite giardia intestinalis: an approach to the control of giardiasis
title Elucidating the roles of annexin-like molecules in the protozoan parasite giardia intestinalis: an approach to the control of giardiasis
title_full Elucidating the roles of annexin-like molecules in the protozoan parasite giardia intestinalis: an approach to the control of giardiasis
title_fullStr Elucidating the roles of annexin-like molecules in the protozoan parasite giardia intestinalis: an approach to the control of giardiasis
title_full_unstemmed Elucidating the roles of annexin-like molecules in the protozoan parasite giardia intestinalis: an approach to the control of giardiasis
title_short Elucidating the roles of annexin-like molecules in the protozoan parasite giardia intestinalis: an approach to the control of giardiasis
title_sort elucidating the roles of annexin-like molecules in the protozoan parasite giardia intestinalis: an approach to the control of giardiasis
topic R Medicine
url http://eprints.usm.my/51603/
http://eprints.usm.my/51603/1/DR.%20CHUAH%20CANDY-Eprints.pdf