Recombinant salmonella typhi ty21a as vaccine candidate against tuberculosis
A recombinant Salmonella Typhi Ty21 a vaccine was developed where the candidate [rSTvaciii(M2)] displaying a multi-epitopes mycobacterial antigen called the Vaciii(M2) (containing ubiquitin and 4 immunogenic mycobacterial epitopes of ESAT-6, PhoSI, Hsp 16.3, and MTB 8.4 genes) on the surface of S...
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| Format: | Thesis |
| Language: | English |
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2008
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| Online Access: | http://eprints.usm.my/51525/ http://eprints.usm.my/51525/1/BADRUL%20SYAM%20BIN%20MAT%20ZAINUDDIN%20-%2024%20pages.pdf |
| _version_ | 1848882010495385600 |
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| author | Badrul Syam, Mat Zainuddin |
| author_facet | Badrul Syam, Mat Zainuddin |
| author_sort | Badrul Syam, Mat Zainuddin |
| building | USM Institutional Repository |
| collection | Online Access |
| description | A recombinant Salmonella Typhi Ty21 a vaccine was developed where the candidate
[rSTvaciii(M2)] displaying a multi-epitopes mycobacterial antigen called the
Vaciii(M2) (containing ubiquitin and 4 immunogenic mycobacterial epitopes of
ESAT-6, PhoSI, Hsp 16.3, and MTB 8.4 genes) on the surface of Salmonella Typhi
Ty21a (Ty2la) using PgsA protein as a carrier. The expression of Vaciii(M2)
protein on the surface of Ty21 a was verified by Western blot. A DNA vaccine was
constructed namely as pVaxVaclll, encoding the Vaclll gene. Immunization in
BALB/c mice with pVaxVaclll and rSTvaciii(M2) either as homologous or
heterologous vaccination, induced strong Th 1-type responses based on high levels of
in vitro IFN-y but low levels of IL-4 secretion. However, the heterologous primeboost
vaccination using pVaxVaclll followed by rSTvaciii(M2) induced lower IFNy
production compared to homologous rSTvaciii(M2) or pVaxVaclll vaccination.
Since the protective immunity against tuberculosis required Th 1-type response, these
rSTvaclii(M2) and pVaxVaclll have potential as effective vaccine candidates
against tuberculosis. |
| first_indexed | 2025-11-15T18:28:07Z |
| format | Thesis |
| id | usm-51525 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T18:28:07Z |
| publishDate | 2008 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-515252022-02-15T01:38:06Z http://eprints.usm.my/51525/ Recombinant salmonella typhi ty21a as vaccine candidate against tuberculosis Badrul Syam, Mat Zainuddin R Medicine (General) A recombinant Salmonella Typhi Ty21 a vaccine was developed where the candidate [rSTvaciii(M2)] displaying a multi-epitopes mycobacterial antigen called the Vaciii(M2) (containing ubiquitin and 4 immunogenic mycobacterial epitopes of ESAT-6, PhoSI, Hsp 16.3, and MTB 8.4 genes) on the surface of Salmonella Typhi Ty21a (Ty2la) using PgsA protein as a carrier. The expression of Vaciii(M2) protein on the surface of Ty21 a was verified by Western blot. A DNA vaccine was constructed namely as pVaxVaclll, encoding the Vaclll gene. Immunization in BALB/c mice with pVaxVaclll and rSTvaciii(M2) either as homologous or heterologous vaccination, induced strong Th 1-type responses based on high levels of in vitro IFN-y but low levels of IL-4 secretion. However, the heterologous primeboost vaccination using pVaxVaclll followed by rSTvaciii(M2) induced lower IFNy production compared to homologous rSTvaciii(M2) or pVaxVaclll vaccination. Since the protective immunity against tuberculosis required Th 1-type response, these rSTvaclii(M2) and pVaxVaclll have potential as effective vaccine candidates against tuberculosis. 2008-10 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/51525/1/BADRUL%20SYAM%20BIN%20MAT%20ZAINUDDIN%20-%2024%20pages.pdf Badrul Syam, Mat Zainuddin (2008) Recombinant salmonella typhi ty21a as vaccine candidate against tuberculosis. Masters thesis, Universiti Sains Malaysia. |
| spellingShingle | R Medicine (General) Badrul Syam, Mat Zainuddin Recombinant salmonella typhi ty21a as vaccine candidate against tuberculosis |
| title | Recombinant salmonella typhi ty21a as
vaccine candidate against
tuberculosis |
| title_full | Recombinant salmonella typhi ty21a as
vaccine candidate against
tuberculosis |
| title_fullStr | Recombinant salmonella typhi ty21a as
vaccine candidate against
tuberculosis |
| title_full_unstemmed | Recombinant salmonella typhi ty21a as
vaccine candidate against
tuberculosis |
| title_short | Recombinant salmonella typhi ty21a as
vaccine candidate against
tuberculosis |
| title_sort | recombinant salmonella typhi ty21a as
vaccine candidate against
tuberculosis |
| topic | R Medicine (General) |
| url | http://eprints.usm.my/51525/ http://eprints.usm.my/51525/1/BADRUL%20SYAM%20BIN%20MAT%20ZAINUDDIN%20-%2024%20pages.pdf |