Screening of antiproliferative activity of artocarpus sp. towards hela cell line and caov-3 cell line
Artocarpus sp. has been used as traditional folk medicine in Southeast Asia for treating ulcer, abscess, and diarrhea. This species also been reported to be used for treatment of inflammation, and malarial fever. In this study, three Artocarpus sp. extract were used to treat two types of cancer c...
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| Format: | Monograph |
| Language: | English |
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Universiti Sains Malaysia
2009
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| Online Access: | http://eprints.usm.my/51128/ http://eprints.usm.my/51128/1/KHAIRUN%20NISA%20IBRAHIM%20ASRI%20-%2024%20pages.pdf |
| _version_ | 1848881905322164224 |
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| author | Khairun Nisa, Ibrahim Asri |
| author_facet | Khairun Nisa, Ibrahim Asri |
| author_sort | Khairun Nisa, Ibrahim Asri |
| building | USM Institutional Repository |
| collection | Online Access |
| description | Artocarpus sp. has been used as traditional folk medicine in Southeast Asia for treating
ulcer, abscess, and diarrhea. This species also been reported to be used for treatment of
inflammation, and malarial fever. In this study, three Artocarpus sp. extract were used to
treat two types of cancer cell lines that are cervical cancer cell line (HeLa) and ovarian
cancer cell line (CaOv-3) to screen the antiproliferative activity. The lowest (the best IC50
value) for inhibitory concentration at 50% of cells population (IC50) shows 6.3lJ.1g/mL.
Treatment of CaOv-3 with Artocarpus extract with the lowest ICso value at specific
incubation time that is 24 hr, 48hr, and 72hr resulted in inducing apoptosis have been
determined by Hoechst stain. Result showed the DNA fragmentation event in the treated
cells compared to untreated cells. Treatment of normal cell line, MDCK with the
Artocarpus extract with the lowest IC50 value showed no cytoselective effect in the extract.
However the extract inhibits cell growth at high concentration equal to I OJ.lg/mL. These
results proposed that Artocarpus sp.exhibit antiproliferative activity on HeLa and CaOv-3
which inducing apoptosis in CaOv-3 cells. |
| first_indexed | 2025-11-15T18:26:27Z |
| format | Monograph |
| id | usm-51128 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T18:26:27Z |
| publishDate | 2009 |
| publisher | Universiti Sains Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-511282022-01-11T04:13:36Z http://eprints.usm.my/51128/ Screening of antiproliferative activity of artocarpus sp. towards hela cell line and caov-3 cell line Khairun Nisa, Ibrahim Asri R Medicine (General) Artocarpus sp. has been used as traditional folk medicine in Southeast Asia for treating ulcer, abscess, and diarrhea. This species also been reported to be used for treatment of inflammation, and malarial fever. In this study, three Artocarpus sp. extract were used to treat two types of cancer cell lines that are cervical cancer cell line (HeLa) and ovarian cancer cell line (CaOv-3) to screen the antiproliferative activity. The lowest (the best IC50 value) for inhibitory concentration at 50% of cells population (IC50) shows 6.3lJ.1g/mL. Treatment of CaOv-3 with Artocarpus extract with the lowest ICso value at specific incubation time that is 24 hr, 48hr, and 72hr resulted in inducing apoptosis have been determined by Hoechst stain. Result showed the DNA fragmentation event in the treated cells compared to untreated cells. Treatment of normal cell line, MDCK with the Artocarpus extract with the lowest IC50 value showed no cytoselective effect in the extract. However the extract inhibits cell growth at high concentration equal to I OJ.lg/mL. These results proposed that Artocarpus sp.exhibit antiproliferative activity on HeLa and CaOv-3 which inducing apoptosis in CaOv-3 cells. Universiti Sains Malaysia 2009 Monograph NonPeerReviewed application/pdf en http://eprints.usm.my/51128/1/KHAIRUN%20NISA%20IBRAHIM%20ASRI%20-%2024%20pages.pdf Khairun Nisa, Ibrahim Asri (2009) Screening of antiproliferative activity of artocarpus sp. towards hela cell line and caov-3 cell line. Other. Universiti Sains Malaysia. (Submitted) |
| spellingShingle | R Medicine (General) Khairun Nisa, Ibrahim Asri Screening of antiproliferative activity of artocarpus sp. towards hela cell line and caov-3 cell line |
| title | Screening of antiproliferative activity of
artocarpus sp. towards hela cell line and
caov-3 cell line |
| title_full | Screening of antiproliferative activity of
artocarpus sp. towards hela cell line and
caov-3 cell line |
| title_fullStr | Screening of antiproliferative activity of
artocarpus sp. towards hela cell line and
caov-3 cell line |
| title_full_unstemmed | Screening of antiproliferative activity of
artocarpus sp. towards hela cell line and
caov-3 cell line |
| title_short | Screening of antiproliferative activity of
artocarpus sp. towards hela cell line and
caov-3 cell line |
| title_sort | screening of antiproliferative activity of
artocarpus sp. towards hela cell line and
caov-3 cell line |
| topic | R Medicine (General) |
| url | http://eprints.usm.my/51128/ http://eprints.usm.my/51128/1/KHAIRUN%20NISA%20IBRAHIM%20ASRI%20-%2024%20pages.pdf |