The separation of phytochemical compounds of spica prunellae and pericarpium of citrus reticula tae by using a reverse phase hplc method
A reversed-phase HPLC-UV method was developed for the separation of phytochemicals in water, n-hexane, and methanol extracts of two of the very common used Chinese herbal medicine, Spica prunellae and pericarpium of Citrus reticulatae. The phytochemical components of these extracts were separated...
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| Format: | Monograph |
| Language: | English |
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Universiti Sains Malaysia
2009
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| Online Access: | http://eprints.usm.my/51106/ http://eprints.usm.my/51106/1/GAN%20CHIN%20CHEAR%20-%2024%20pages.pdf |
| _version_ | 1848881899775197184 |
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| author | Gan Chin, Chear |
| author_facet | Gan Chin, Chear |
| author_sort | Gan Chin, Chear |
| building | USM Institutional Repository |
| collection | Online Access |
| description | A reversed-phase HPLC-UV method was developed for the separation of
phytochemicals in water, n-hexane, and methanol extracts of two of the very common
used Chinese herbal medicine, Spica prunellae and pericarpium of Citrus reticulatae.
The phytochemical components of these extracts were separated by RP-HPLC on a
C-18 column and adjusting mobile phase (acetonitrile: methanol; 40: 60 v/ v), flow rate
at 0.8 mL/min.
The identification of phytochemicals separated by RP-HPLC with UV-detection at
280 nm and 320 nm is based on the comparison of peak retention times of some
previously published works. However, this needs to be confirmed by the use of standard
samples under the same experimental condition. |
| first_indexed | 2025-11-15T18:26:21Z |
| format | Monograph |
| id | usm-51106 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T18:26:21Z |
| publishDate | 2009 |
| publisher | Universiti Sains Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-511062022-01-09T08:25:47Z http://eprints.usm.my/51106/ The separation of phytochemical compounds of spica prunellae and pericarpium of citrus reticula tae by using a reverse phase hplc method Gan Chin, Chear R Medicine (General) A reversed-phase HPLC-UV method was developed for the separation of phytochemicals in water, n-hexane, and methanol extracts of two of the very common used Chinese herbal medicine, Spica prunellae and pericarpium of Citrus reticulatae. The phytochemical components of these extracts were separated by RP-HPLC on a C-18 column and adjusting mobile phase (acetonitrile: methanol; 40: 60 v/ v), flow rate at 0.8 mL/min. The identification of phytochemicals separated by RP-HPLC with UV-detection at 280 nm and 320 nm is based on the comparison of peak retention times of some previously published works. However, this needs to be confirmed by the use of standard samples under the same experimental condition. Universiti Sains Malaysia 2009 Monograph NonPeerReviewed application/pdf en http://eprints.usm.my/51106/1/GAN%20CHIN%20CHEAR%20-%2024%20pages.pdf Gan Chin, Chear (2009) The separation of phytochemical compounds of spica prunellae and pericarpium of citrus reticula tae by using a reverse phase hplc method. Other. Universiti Sains Malaysia. (Submitted) |
| spellingShingle | R Medicine (General) Gan Chin, Chear The separation of phytochemical compounds of spica prunellae and pericarpium of citrus reticula tae by using a reverse phase hplc method |
| title | The separation of phytochemical compounds of spica prunellae
and pericarpium of citrus reticula tae by using a reverse phase hplc method |
| title_full | The separation of phytochemical compounds of spica prunellae
and pericarpium of citrus reticula tae by using a reverse phase hplc method |
| title_fullStr | The separation of phytochemical compounds of spica prunellae
and pericarpium of citrus reticula tae by using a reverse phase hplc method |
| title_full_unstemmed | The separation of phytochemical compounds of spica prunellae
and pericarpium of citrus reticula tae by using a reverse phase hplc method |
| title_short | The separation of phytochemical compounds of spica prunellae
and pericarpium of citrus reticula tae by using a reverse phase hplc method |
| title_sort | separation of phytochemical compounds of spica prunellae
and pericarpium of citrus reticula tae by using a reverse phase hplc method |
| topic | R Medicine (General) |
| url | http://eprints.usm.my/51106/ http://eprints.usm.my/51106/1/GAN%20CHIN%20CHEAR%20-%2024%20pages.pdf |