Dna fragmentation in hela cells treated with Brucea javanica fruits extract
Fruits of Brucea javanica (B. javanica) have been used as traditional medicine in China as it has been shown to have antimalarial, antiplasmodial, antitumor or anticancer and antiamoebic. In this study, methanol extracts of B. javanica fruit (BJF) were treated on human cervical cancer cell (HeLa)...
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| Format: | Monograph |
| Language: | English |
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Pusat Pengajian Sains Perubatan Universiti Sains Malaysia
2008
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| Online Access: | http://eprints.usm.my/50706/ http://eprints.usm.my/50706/1/NOOR%20HIDAYU%20BINTI%20PATHNI%20-%2024%20pages.pdf |
| _version_ | 1848881793173815296 |
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| author | Noor Hidayu, Pathni |
| author_facet | Noor Hidayu, Pathni |
| author_sort | Noor Hidayu, Pathni |
| building | USM Institutional Repository |
| collection | Online Access |
| description | Fruits of Brucea javanica (B. javanica) have been used as traditional medicine in China as
it has been shown to have antimalarial, antiplasmodial, antitumor or anticancer and
antiamoebic. In this study, methanol extracts of B. javanica fruit (BJF) were treated on
human cervical cancer cell (HeLa). Antiproliferative activity of BJF methanol extracts on
HeLa cells showed ICso value of 13.34 J.l.g/ml. After that, TUNEL assay (Terminal
Deoxynucleotide Transferase dUTP Nick End Labeling) was done to detect the apoptosis
rate of BJF methanol extract on HeLa cells. The apoptosis rate for 24 h, 48 h and 72 h were
13.8%, 17.8% and 22.9% respectively. The percentage of apoptosis rate was time
dependent. These results proposed that BJF methanol extracts exhibits antiproliferative
effect on HeLa cells by inducing apoptosis. Therefore, it may be a potential candidate for
antiproliferative agent for the treatment of human cervical cancer cells. |
| first_indexed | 2025-11-15T18:24:40Z |
| format | Monograph |
| id | usm-50706 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T18:24:40Z |
| publishDate | 2008 |
| publisher | Pusat Pengajian Sains Perubatan Universiti Sains Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-507062021-11-21T08:31:38Z http://eprints.usm.my/50706/ Dna fragmentation in hela cells treated with Brucea javanica fruits extract Noor Hidayu, Pathni R Medicine (General) Fruits of Brucea javanica (B. javanica) have been used as traditional medicine in China as it has been shown to have antimalarial, antiplasmodial, antitumor or anticancer and antiamoebic. In this study, methanol extracts of B. javanica fruit (BJF) were treated on human cervical cancer cell (HeLa). Antiproliferative activity of BJF methanol extracts on HeLa cells showed ICso value of 13.34 J.l.g/ml. After that, TUNEL assay (Terminal Deoxynucleotide Transferase dUTP Nick End Labeling) was done to detect the apoptosis rate of BJF methanol extract on HeLa cells. The apoptosis rate for 24 h, 48 h and 72 h were 13.8%, 17.8% and 22.9% respectively. The percentage of apoptosis rate was time dependent. These results proposed that BJF methanol extracts exhibits antiproliferative effect on HeLa cells by inducing apoptosis. Therefore, it may be a potential candidate for antiproliferative agent for the treatment of human cervical cancer cells. Pusat Pengajian Sains Perubatan Universiti Sains Malaysia 2008 Monograph NonPeerReviewed application/pdf en http://eprints.usm.my/50706/1/NOOR%20HIDAYU%20BINTI%20PATHNI%20-%2024%20pages.pdf Noor Hidayu, Pathni (2008) Dna fragmentation in hela cells treated with Brucea javanica fruits extract. Other. Pusat Pengajian Sains Perubatan Universiti Sains Malaysia. (Submitted) |
| spellingShingle | R Medicine (General) Noor Hidayu, Pathni Dna fragmentation in hela cells treated with Brucea javanica fruits extract |
| title | Dna fragmentation in hela cells treated with Brucea
javanica fruits extract |
| title_full | Dna fragmentation in hela cells treated with Brucea
javanica fruits extract |
| title_fullStr | Dna fragmentation in hela cells treated with Brucea
javanica fruits extract |
| title_full_unstemmed | Dna fragmentation in hela cells treated with Brucea
javanica fruits extract |
| title_short | Dna fragmentation in hela cells treated with Brucea
javanica fruits extract |
| title_sort | dna fragmentation in hela cells treated with brucea
javanica fruits extract |
| topic | R Medicine (General) |
| url | http://eprints.usm.my/50706/ http://eprints.usm.my/50706/1/NOOR%20HIDAYU%20BINTI%20PATHNI%20-%2024%20pages.pdf |