Determining the significance of coagulase-negative Staphylococci (CoNS) from blood cultures by molecular approach
Background: Coagulase-negative staphylococci (CoNS) are a group of micro-organisms that are increasingly implicated as a cause of significant infection and the leading cause of blood stream infection (BSI). One important predictor of true BSI is the isolation of CoNS from multiple blood cultures,...
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| Format: | Article |
| Language: | English |
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Pusat Pengajian Sains Perubatan, Universiti Sains Malaysia
2011
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| Online Access: | http://eprints.usm.my/50656/ http://eprints.usm.my/50656/1/DR.%20ZAIDAH%20ABDUL%20RAHMAN-Eprints.pdf |
| Summary: | Background: Coagulase-negative staphylococci (CoNS) are a group of micro-organisms
that are increasingly implicated as a cause of significant infection and the leading cause
of blood stream infection (BSI). One important predictor of true BSI is the isolation of
CoNS from multiple blood cultures, presuming that the isolates represent the same
species.
Aim: Thus, the objective of this study was to determine the significance of repeated
CoNS isolated from blood cultures.
Methods: This was a prospective laboratory study which was initiated in June 2007 until
July 2008. CoNS isolates were obtained from patients who had two positive blood
cultures within a 14-day interval. CoNS were identified with the species level using an
API-Staph and antibiotics susceptibility testing was performed according to the CLSI
standard. Strain relatedness was confirmed using pulsed-field gel electrophoresis.
Findings: During the study period, 202 of CoNS were isolated from I 01 patients. The
most common species isolated was Staphylococcus epidermidis (59.0%). 83.2% of
patients isolated same species of Co NS from repeated blood cultures. Among the isolates
of same species, only 40. 7% had the same antibiogram. CoNS with the same species and
antibiogram had 93.3% probability of the same strain. 65.5% of patients were treated
with antibiotics, especially glycopeptides group.
Conclusion: Speciation and antibiogram of CoNS from repeated blood cultures are
adequate in determining the significance of repeated Co NS isolated from blood cultures. |
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