Study on the effect of black cumin Oil and LDL on lipopol ysaccharide (lps) - induced toll - like receptor activation in macrophage and dendritic cell lines
Lipid-laden macrophage has been reported to play various roles in atherogenesis. The focus was to elucidate effects of a natural product on the progression of monocytederived macrophage growth. Nigella saliva was selected as a form of treatment to macrophage cell growth in culture conditions due...
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| Format: | Article |
| Language: | English |
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Pusat Pengajian Sains Perubatan, Universiti Sains Malaysia
2011
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| Online Access: | http://eprints.usm.my/50342/ http://eprints.usm.my/50342/1/DR.%20SHAHRUL%20BARIYAH%20SAHUL%20HAMID-EPRINTS.pdf |
| _version_ | 1848881696284344320 |
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| author | Hamid, Shahrul Bariyah Sahul |
| author_facet | Hamid, Shahrul Bariyah Sahul |
| author_sort | Hamid, Shahrul Bariyah Sahul |
| building | USM Institutional Repository |
| collection | Online Access |
| description | Lipid-laden macrophage has been reported to play various roles in atherogenesis. The
focus was to elucidate effects of a natural product on the progression of monocytederived
macrophage growth. Nigella saliva was selected as a form of treatment to
macrophage cell growth in culture conditions due to various findings on its medical
benefits. Human monocytes were isolated and grown at 37°C and 5%C02 saturation for
5 days prior to treatment with Nigella sativa oil. The cells were plated and washed before
addition of ox-LDL (lOμg/ml) alone in untreated condition and combination of ox-LDL
(lOμg/ml) and (72 μg/ml) Nigella sativa oil in treated condition. The growth progression
was monitored every 24 hours for 3 days. Nigella sativa oil caused noticeble effect on
macrophage growth compared to monocyte especially 24 hours after treatment. The mean
was significant different between untreated and treated condition for both monocytes and
macrophages (p<0.001). This was shown by the delayed growth pattern as seen in
macrophage compared to monocytes. There was less oil red 0 staining in cells treated
with mixture of oxLDL and Nigella sativa compared to those treated with oxLDL alone.
The signalling of Nigella saliva has been reported to occur via TLR-4 receptor, where it
is significantly present on macrophage cell surface. This may be one of the factors
leading to the growth difference. Hence, progression of macrophage to foam cells could
possibly be controlled with the use of Nigella saliva oil. |
| first_indexed | 2025-11-15T18:23:07Z |
| format | Article |
| id | usm-50342 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T18:23:07Z |
| publishDate | 2011 |
| publisher | Pusat Pengajian Sains Perubatan, Universiti Sains Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-503422021-10-21T15:43:54Z http://eprints.usm.my/50342/ Study on the effect of black cumin Oil and LDL on lipopol ysaccharide (lps) - induced toll - like receptor activation in macrophage and dendritic cell lines Hamid, Shahrul Bariyah Sahul R Medicine Lipid-laden macrophage has been reported to play various roles in atherogenesis. The focus was to elucidate effects of a natural product on the progression of monocytederived macrophage growth. Nigella saliva was selected as a form of treatment to macrophage cell growth in culture conditions due to various findings on its medical benefits. Human monocytes were isolated and grown at 37°C and 5%C02 saturation for 5 days prior to treatment with Nigella sativa oil. The cells were plated and washed before addition of ox-LDL (lOμg/ml) alone in untreated condition and combination of ox-LDL (lOμg/ml) and (72 μg/ml) Nigella sativa oil in treated condition. The growth progression was monitored every 24 hours for 3 days. Nigella sativa oil caused noticeble effect on macrophage growth compared to monocyte especially 24 hours after treatment. The mean was significant different between untreated and treated condition for both monocytes and macrophages (p<0.001). This was shown by the delayed growth pattern as seen in macrophage compared to monocytes. There was less oil red 0 staining in cells treated with mixture of oxLDL and Nigella sativa compared to those treated with oxLDL alone. The signalling of Nigella saliva has been reported to occur via TLR-4 receptor, where it is significantly present on macrophage cell surface. This may be one of the factors leading to the growth difference. Hence, progression of macrophage to foam cells could possibly be controlled with the use of Nigella saliva oil. Pusat Pengajian Sains Perubatan, Universiti Sains Malaysia 2011 Article NonPeerReviewed application/pdf en http://eprints.usm.my/50342/1/DR.%20SHAHRUL%20BARIYAH%20SAHUL%20HAMID-EPRINTS.pdf Hamid, Shahrul Bariyah Sahul (2011) Study on the effect of black cumin Oil and LDL on lipopol ysaccharide (lps) - induced toll - like receptor activation in macrophage and dendritic cell lines. Study on the effect of black cumin Oil and LDL on lipopol ysaccharide (lps) - induced toll - like receptor activation in macrophage and dendritic cell lines. |
| spellingShingle | R Medicine Hamid, Shahrul Bariyah Sahul Study on the effect of black cumin Oil and LDL on lipopol ysaccharide (lps) - induced toll - like receptor activation in macrophage and dendritic cell lines |
| title | Study on the effect of black cumin
Oil and LDL on lipopol ysaccharide
(lps) - induced toll - like receptor
activation in macrophage and
dendritic cell lines |
| title_full | Study on the effect of black cumin
Oil and LDL on lipopol ysaccharide
(lps) - induced toll - like receptor
activation in macrophage and
dendritic cell lines |
| title_fullStr | Study on the effect of black cumin
Oil and LDL on lipopol ysaccharide
(lps) - induced toll - like receptor
activation in macrophage and
dendritic cell lines |
| title_full_unstemmed | Study on the effect of black cumin
Oil and LDL on lipopol ysaccharide
(lps) - induced toll - like receptor
activation in macrophage and
dendritic cell lines |
| title_short | Study on the effect of black cumin
Oil and LDL on lipopol ysaccharide
(lps) - induced toll - like receptor
activation in macrophage and
dendritic cell lines |
| title_sort | study on the effect of black cumin
oil and ldl on lipopol ysaccharide
(lps) - induced toll - like receptor
activation in macrophage and
dendritic cell lines |
| topic | R Medicine |
| url | http://eprints.usm.my/50342/ http://eprints.usm.my/50342/1/DR.%20SHAHRUL%20BARIYAH%20SAHUL%20HAMID-EPRINTS.pdf |