Hypervariable segment-! (HVS-I) Sequence Analysis Of Chinese Population of Peninsular Malaysia

Throughout Malaysia history, most of the Peninsular Malaysian Chinese are known to be populated by those migrated from the China during the British colonial era (Barbie, 1992). The present study was undertaken to obtain information about the distribution of the existing HVS-1 sequence variations...

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Main Author: Lee Loong, Chuen
Format: Monograph
Language:English
Published: PPSP, Universiti Sains Malaysia 2007
Subjects:
Online Access:http://eprints.usm.my/50207/
http://eprints.usm.my/50207/1/LEE%20LOONG%20CHUEN%20-%2024%20pages.pdf
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author Lee Loong, Chuen
author_facet Lee Loong, Chuen
author_sort Lee Loong, Chuen
building USM Institutional Repository
collection Online Access
description Throughout Malaysia history, most of the Peninsular Malaysian Chinese are known to be populated by those migrated from the China during the British colonial era (Barbie, 1992). The present study was undertaken to obtain information about the distribution of the existing HVS-1 sequence variations in the Chinese population of Peninsular Malaysia. A few studies have used RFLP analysis to characterize the mtDNA of Malaysian Chinese (Ismail, 2006; Mokhtaruddin, 2006; Ballinger et al., 1992). The primary purpose of this study is to provide HVS-1 sequence data by sequencing the HVS-1 of the 40 Chinese individuals from different parts of Peninsular Malaysia. The secondary purpose is classification of mtDNA haplogroup based on the HVS-1 sequence variation by alignment of samples sequence and Cambridge Reference Sequence (CRS) (Andrew et al., 1999). The samples were predicted to belong to the superhaplogroup M and haplogroup B in relatively high frequency as inferred from the study done by Ballinger et al., (1992). Analysis of HVS-1 within the control region (CR) of the mitochondrial genome revealed the presence of eight haplogroups: B, F, M, MC, MD, MG, N, and R. These haplogroups are the characteristics of Southeast Asian populations (Ballinger et al., 1992) and also found in significant proportion in Han Chinese of China (Y ao et al., 2002). Result shows that superhaplogroup M (includes MC, MD, and MG) is the most common haplogroup in the studied samples (55%), followed by haplogroup B (18%).
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spelling usm-502072021-10-11T02:48:28Z http://eprints.usm.my/50207/ Hypervariable segment-! (HVS-I) Sequence Analysis Of Chinese Population of Peninsular Malaysia Lee Loong, Chuen R Medicine (General) Throughout Malaysia history, most of the Peninsular Malaysian Chinese are known to be populated by those migrated from the China during the British colonial era (Barbie, 1992). The present study was undertaken to obtain information about the distribution of the existing HVS-1 sequence variations in the Chinese population of Peninsular Malaysia. A few studies have used RFLP analysis to characterize the mtDNA of Malaysian Chinese (Ismail, 2006; Mokhtaruddin, 2006; Ballinger et al., 1992). The primary purpose of this study is to provide HVS-1 sequence data by sequencing the HVS-1 of the 40 Chinese individuals from different parts of Peninsular Malaysia. The secondary purpose is classification of mtDNA haplogroup based on the HVS-1 sequence variation by alignment of samples sequence and Cambridge Reference Sequence (CRS) (Andrew et al., 1999). The samples were predicted to belong to the superhaplogroup M and haplogroup B in relatively high frequency as inferred from the study done by Ballinger et al., (1992). Analysis of HVS-1 within the control region (CR) of the mitochondrial genome revealed the presence of eight haplogroups: B, F, M, MC, MD, MG, N, and R. These haplogroups are the characteristics of Southeast Asian populations (Ballinger et al., 1992) and also found in significant proportion in Han Chinese of China (Y ao et al., 2002). Result shows that superhaplogroup M (includes MC, MD, and MG) is the most common haplogroup in the studied samples (55%), followed by haplogroup B (18%). PPSP, Universiti Sains Malaysia 2007-04 Monograph NonPeerReviewed application/pdf en http://eprints.usm.my/50207/1/LEE%20LOONG%20CHUEN%20-%2024%20pages.pdf Lee Loong, Chuen (2007) Hypervariable segment-! (HVS-I) Sequence Analysis Of Chinese Population of Peninsular Malaysia. Other. PPSP, Universiti Sains Malaysia. (Submitted)
spellingShingle R Medicine (General)
Lee Loong, Chuen
Hypervariable segment-! (HVS-I) Sequence Analysis Of Chinese Population of Peninsular Malaysia
title Hypervariable segment-! (HVS-I) Sequence Analysis Of Chinese Population of Peninsular Malaysia
title_full Hypervariable segment-! (HVS-I) Sequence Analysis Of Chinese Population of Peninsular Malaysia
title_fullStr Hypervariable segment-! (HVS-I) Sequence Analysis Of Chinese Population of Peninsular Malaysia
title_full_unstemmed Hypervariable segment-! (HVS-I) Sequence Analysis Of Chinese Population of Peninsular Malaysia
title_short Hypervariable segment-! (HVS-I) Sequence Analysis Of Chinese Population of Peninsular Malaysia
title_sort hypervariable segment-! (hvs-i) sequence analysis of chinese population of peninsular malaysia
topic R Medicine (General)
url http://eprints.usm.my/50207/
http://eprints.usm.my/50207/1/LEE%20LOONG%20CHUEN%20-%2024%20pages.pdf