The Effects Of Mitragynine From Mitragyna Speciosa On The Mrna Expression Of Cox-1 And Cox-2 In Lipopolysaccharide-Stimulated Raw264.7 Macrophage Cells
Mitragyna speciosa Korth (Rubiaceae) is one of the medicinal plants used traditionally to treat various types of diseases especially in Thailand and Malaysia. Its anti-inflammatory and analgesic properties of its crude form are well documented. In this study, the cellular mechanism involved in th...
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| Format: | Thesis |
| Language: | English |
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2014
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| Online Access: | http://eprints.usm.my/50168/ http://eprints.usm.my/50168/1/Zulkhurnain%20Bin%20Utar24.pdf |
| _version_ | 1848881650368249856 |
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| author | Utar, Zulkhurnain |
| author_facet | Utar, Zulkhurnain |
| author_sort | Utar, Zulkhurnain |
| building | USM Institutional Repository |
| collection | Online Access |
| description | Mitragyna speciosa Korth (Rubiaceae) is one of the medicinal plants used
traditionally to treat various types of diseases especially in Thailand and Malaysia.
Its anti-inflammatory and analgesic properties of its crude form are well documented.
In this study, the cellular mechanism involved in the anti-inflammatory effects of
mitragynine, the major bioactive constituent, was investigated. The effects of
mitragynine on the mRNA and protein expression of COX-1 and COX-2 and the
production of prostaglandin E2 (PGE2) were investigated in LPS-treated RAW264.7
macrophage cells. Quantitative RT-PCR was used to assess the mRNA expression of
COX-1 and COX-2. Protein expression of COX-1 and COX-2 were assessed using
Western blot analysis and the level of PGE2 production was quantified using
Parameter™ PGE2 Assay (R&D Systems). |
| first_indexed | 2025-11-15T18:22:23Z |
| format | Thesis |
| id | usm-50168 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T18:22:23Z |
| publishDate | 2014 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-501682021-10-06T05:42:39Z http://eprints.usm.my/50168/ The Effects Of Mitragynine From Mitragyna Speciosa On The Mrna Expression Of Cox-1 And Cox-2 In Lipopolysaccharide-Stimulated Raw264.7 Macrophage Cells Utar, Zulkhurnain RK1-715 Dentistry Mitragyna speciosa Korth (Rubiaceae) is one of the medicinal plants used traditionally to treat various types of diseases especially in Thailand and Malaysia. Its anti-inflammatory and analgesic properties of its crude form are well documented. In this study, the cellular mechanism involved in the anti-inflammatory effects of mitragynine, the major bioactive constituent, was investigated. The effects of mitragynine on the mRNA and protein expression of COX-1 and COX-2 and the production of prostaglandin E2 (PGE2) were investigated in LPS-treated RAW264.7 macrophage cells. Quantitative RT-PCR was used to assess the mRNA expression of COX-1 and COX-2. Protein expression of COX-1 and COX-2 were assessed using Western blot analysis and the level of PGE2 production was quantified using Parameter™ PGE2 Assay (R&D Systems). 2014-01 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/50168/1/Zulkhurnain%20Bin%20Utar24.pdf Utar, Zulkhurnain (2014) The Effects Of Mitragynine From Mitragyna Speciosa On The Mrna Expression Of Cox-1 And Cox-2 In Lipopolysaccharide-Stimulated Raw264.7 Macrophage Cells. Masters thesis, Universiti Sains Malaysia. |
| spellingShingle | RK1-715 Dentistry Utar, Zulkhurnain The Effects Of Mitragynine From Mitragyna Speciosa On The Mrna Expression Of Cox-1 And Cox-2 In Lipopolysaccharide-Stimulated Raw264.7 Macrophage Cells |
| title | The Effects Of Mitragynine From
Mitragyna Speciosa On The Mrna
Expression Of Cox-1 And Cox-2 In
Lipopolysaccharide-Stimulated
Raw264.7 Macrophage Cells |
| title_full | The Effects Of Mitragynine From
Mitragyna Speciosa On The Mrna
Expression Of Cox-1 And Cox-2 In
Lipopolysaccharide-Stimulated
Raw264.7 Macrophage Cells |
| title_fullStr | The Effects Of Mitragynine From
Mitragyna Speciosa On The Mrna
Expression Of Cox-1 And Cox-2 In
Lipopolysaccharide-Stimulated
Raw264.7 Macrophage Cells |
| title_full_unstemmed | The Effects Of Mitragynine From
Mitragyna Speciosa On The Mrna
Expression Of Cox-1 And Cox-2 In
Lipopolysaccharide-Stimulated
Raw264.7 Macrophage Cells |
| title_short | The Effects Of Mitragynine From
Mitragyna Speciosa On The Mrna
Expression Of Cox-1 And Cox-2 In
Lipopolysaccharide-Stimulated
Raw264.7 Macrophage Cells |
| title_sort | effects of mitragynine from
mitragyna speciosa on the mrna
expression of cox-1 and cox-2 in
lipopolysaccharide-stimulated
raw264.7 macrophage cells |
| topic | RK1-715 Dentistry |
| url | http://eprints.usm.my/50168/ http://eprints.usm.my/50168/1/Zulkhurnain%20Bin%20Utar24.pdf |