Evaluation of aqueous extract of syzygium polyanthum leaves as anti-hypertensive agent in spontaneous hypertensive rat (SHR)
Hypertension is associated with significant morbidity and mortality. The use of medicinal herbs as alternative medicines to manage hypertension is increasing exponentially. Syzygium polyanthum, has been claimed traditionally as an antihypertensive agent. This study aimed to determine the antihype...
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| Format: | Thesis |
| Language: | English |
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2021
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| Online Access: | http://eprints.usm.my/49881/ http://eprints.usm.my/49881/1/NURUL%20SYAHIDA%20BINTI%20RAMLI-FINAL%20THESIS%20P-SKD001217%28R%29%20PWD_-24%20pages.pdf |
| _version_ | 1848881570655502336 |
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| author | Ramli, Nurul Syahida |
| author_facet | Ramli, Nurul Syahida |
| author_sort | Ramli, Nurul Syahida |
| building | USM Institutional Repository |
| collection | Online Access |
| description | Hypertension is associated with significant morbidity and mortality. The use
of medicinal herbs as alternative medicines to manage hypertension is increasing
exponentially. Syzygium polyanthum, has been claimed traditionally as an
antihypertensive agent. This study aimed to determine the antihypertensive effects of
the aqueous extract of S. polyanthum (AESP) leaves and its mechanisms using
spontaneously hypertensive rats (SHR). The phytochemical profiling, antioxidant
properties and antihypertensive activity were evaluated. Fifty male SHR were divided
equally into 5 groups; untreated-SHR, Losartan-treated, 3 groups of AESP-treated
with different dosages (1500 mg/kg, 1750 mg/kg and 2250 mg/kg), and 10 male WKY
rats as control. All treatments were given orally for 12th weeks. Systolic blood pressure
(SBP) was measured biweekly. Whereas, the biochemical analysis, oxidative stress
markers and angiotensin-converting enzyme (ACE) level were evaluated at the end of
the study. The histology of thoracic aorta and kidney were assessed using
haematoxylin & eosin (H&E) staining, and scanning electron microscope (SEM).
AESP contains flavonoids and phenols with gallic acid detected. AESP showed high
in vitro antioxidant and ACE-inhibitory activities. In AESP-treated SHR; SBP reduced
significantly, improved renal function and oxidative stress markers. However, only
AESP (2250 mg/kg) significantly reduced ACE concentration. There was also
histology improvement of the thoracic aorta and renal in AESP-treated SHR. Hence,
the study suggests that antihypertensive properties of AESP are due to its antioxidant(mainly gallic acid) and ACE inhibitory activity. Thus, this study reveals the
antihypertensive mechanism of AESP exerted inhibitory activity through suppression
of the ACE action which might involved RAAS pathway. |
| first_indexed | 2025-11-15T18:21:07Z |
| format | Thesis |
| id | usm-49881 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T18:21:07Z |
| publishDate | 2021 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-498812021-09-12T03:01:38Z http://eprints.usm.my/49881/ Evaluation of aqueous extract of syzygium polyanthum leaves as anti-hypertensive agent in spontaneous hypertensive rat (SHR) Ramli, Nurul Syahida R Medicine Hypertension is associated with significant morbidity and mortality. The use of medicinal herbs as alternative medicines to manage hypertension is increasing exponentially. Syzygium polyanthum, has been claimed traditionally as an antihypertensive agent. This study aimed to determine the antihypertensive effects of the aqueous extract of S. polyanthum (AESP) leaves and its mechanisms using spontaneously hypertensive rats (SHR). The phytochemical profiling, antioxidant properties and antihypertensive activity were evaluated. Fifty male SHR were divided equally into 5 groups; untreated-SHR, Losartan-treated, 3 groups of AESP-treated with different dosages (1500 mg/kg, 1750 mg/kg and 2250 mg/kg), and 10 male WKY rats as control. All treatments were given orally for 12th weeks. Systolic blood pressure (SBP) was measured biweekly. Whereas, the biochemical analysis, oxidative stress markers and angiotensin-converting enzyme (ACE) level were evaluated at the end of the study. The histology of thoracic aorta and kidney were assessed using haematoxylin & eosin (H&E) staining, and scanning electron microscope (SEM). AESP contains flavonoids and phenols with gallic acid detected. AESP showed high in vitro antioxidant and ACE-inhibitory activities. In AESP-treated SHR; SBP reduced significantly, improved renal function and oxidative stress markers. However, only AESP (2250 mg/kg) significantly reduced ACE concentration. There was also histology improvement of the thoracic aorta and renal in AESP-treated SHR. Hence, the study suggests that antihypertensive properties of AESP are due to its antioxidant(mainly gallic acid) and ACE inhibitory activity. Thus, this study reveals the antihypertensive mechanism of AESP exerted inhibitory activity through suppression of the ACE action which might involved RAAS pathway. 2021-05 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/49881/1/NURUL%20SYAHIDA%20BINTI%20RAMLI-FINAL%20THESIS%20P-SKD001217%28R%29%20PWD_-24%20pages.pdf Ramli, Nurul Syahida (2021) Evaluation of aqueous extract of syzygium polyanthum leaves as anti-hypertensive agent in spontaneous hypertensive rat (SHR). PhD thesis, Universiti Sains Malaysia. |
| spellingShingle | R Medicine Ramli, Nurul Syahida Evaluation of aqueous extract of syzygium polyanthum leaves as anti-hypertensive agent in spontaneous hypertensive rat (SHR) |
| title | Evaluation of aqueous extract of syzygium polyanthum leaves as anti-hypertensive agent in spontaneous hypertensive rat (SHR) |
| title_full | Evaluation of aqueous extract of syzygium polyanthum leaves as anti-hypertensive agent in spontaneous hypertensive rat (SHR) |
| title_fullStr | Evaluation of aqueous extract of syzygium polyanthum leaves as anti-hypertensive agent in spontaneous hypertensive rat (SHR) |
| title_full_unstemmed | Evaluation of aqueous extract of syzygium polyanthum leaves as anti-hypertensive agent in spontaneous hypertensive rat (SHR) |
| title_short | Evaluation of aqueous extract of syzygium polyanthum leaves as anti-hypertensive agent in spontaneous hypertensive rat (SHR) |
| title_sort | evaluation of aqueous extract of syzygium polyanthum leaves as anti-hypertensive agent in spontaneous hypertensive rat (shr) |
| topic | R Medicine |
| url | http://eprints.usm.my/49881/ http://eprints.usm.my/49881/1/NURUL%20SYAHIDA%20BINTI%20RAMLI-FINAL%20THESIS%20P-SKD001217%28R%29%20PWD_-24%20pages.pdf |