Production Of Novel Recombinant Antipfhrp2 Vnar-G1 Protein Using Escherichia Coli Bl21(De3) Expression System
Malaria rapid diagnostic tests (RDTs) act as important antibody-based immunoassays for prompt malaria diagnosis. Conventional monoclonal antibodies (mAbs) are widely used in RDTs but it can be easily degraded at high ambient temperatures. Hence, the shark VNARS might be good alternatives to mAbs du...
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| Format: | Monograph |
| Language: | English |
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Universiti Sains Malaysia
2020
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| Online Access: | http://eprints.usm.my/49503/ http://eprints.usm.my/49503/1/Kok%20Boon%20Hui.pdf%20cut.pdf |
| _version_ | 1848881456518004736 |
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| author | Kok, Boon Hui |
| author_facet | Kok, Boon Hui |
| author_sort | Kok, Boon Hui |
| building | USM Institutional Repository |
| collection | Online Access |
| description | Malaria rapid diagnostic tests (RDTs) act as important antibody-based immunoassays for prompt malaria diagnosis. Conventional monoclonal antibodies (mAbs) are
widely used in RDTs but it can be easily degraded at high ambient temperatures. Hence, the shark VNARS might be good alternatives to mAbs due to its higher thermal
stability and binding affinity with antigens. In this study, the recombinant anti- PfHRP2 VNAR-G1 protein was produced in E. coli expression system through various
steps such as recombinant cell isolation, PCR, agarose gel electrophoresis, plasmid extraction, transformation and protein expression. Besides, the combinatorial effects of temperature and IPTG concentration towards the cell density of recombinant BL21(DE3) based on the absorbance readings and cell wet weights were investigated using software R. |
| first_indexed | 2025-11-15T18:19:19Z |
| format | Monograph |
| id | usm-49503 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T18:19:19Z |
| publishDate | 2020 |
| publisher | Universiti Sains Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-495032021-07-28T01:44:07Z http://eprints.usm.my/49503/ Production Of Novel Recombinant Antipfhrp2 Vnar-G1 Protein Using Escherichia Coli Bl21(De3) Expression System Kok, Boon Hui T1-995 Technology(General) Malaria rapid diagnostic tests (RDTs) act as important antibody-based immunoassays for prompt malaria diagnosis. Conventional monoclonal antibodies (mAbs) are widely used in RDTs but it can be easily degraded at high ambient temperatures. Hence, the shark VNARS might be good alternatives to mAbs due to its higher thermal stability and binding affinity with antigens. In this study, the recombinant anti- PfHRP2 VNAR-G1 protein was produced in E. coli expression system through various steps such as recombinant cell isolation, PCR, agarose gel electrophoresis, plasmid extraction, transformation and protein expression. Besides, the combinatorial effects of temperature and IPTG concentration towards the cell density of recombinant BL21(DE3) based on the absorbance readings and cell wet weights were investigated using software R. Universiti Sains Malaysia 2020-06 Monograph NonPeerReviewed application/pdf en http://eprints.usm.my/49503/1/Kok%20Boon%20Hui.pdf%20cut.pdf Kok, Boon Hui (2020) Production Of Novel Recombinant Antipfhrp2 Vnar-G1 Protein Using Escherichia Coli Bl21(De3) Expression System. Other. Universiti Sains Malaysia. (Submitted) |
| spellingShingle | T1-995 Technology(General) Kok, Boon Hui Production Of Novel Recombinant Antipfhrp2 Vnar-G1 Protein Using Escherichia Coli Bl21(De3) Expression System |
| title | Production Of Novel Recombinant Antipfhrp2
Vnar-G1 Protein Using Escherichia Coli Bl21(De3) Expression System |
| title_full | Production Of Novel Recombinant Antipfhrp2
Vnar-G1 Protein Using Escherichia Coli Bl21(De3) Expression System |
| title_fullStr | Production Of Novel Recombinant Antipfhrp2
Vnar-G1 Protein Using Escherichia Coli Bl21(De3) Expression System |
| title_full_unstemmed | Production Of Novel Recombinant Antipfhrp2
Vnar-G1 Protein Using Escherichia Coli Bl21(De3) Expression System |
| title_short | Production Of Novel Recombinant Antipfhrp2
Vnar-G1 Protein Using Escherichia Coli Bl21(De3) Expression System |
| title_sort | production of novel recombinant antipfhrp2
vnar-g1 protein using escherichia coli bl21(de3) expression system |
| topic | T1-995 Technology(General) |
| url | http://eprints.usm.my/49503/ http://eprints.usm.my/49503/1/Kok%20Boon%20Hui.pdf%20cut.pdf |