The expression of recombinant bacille calmette-guerin (rBCG) containing VPI gene of enterovirus 71 (EV71)
Enterovirus 71 (EV71) is a positive-sense single-stranded RNA, nonenveloped, icosahedrally symmetrical virus which was first detected in 1969 in United States. EV71 is associated with Hand-Foot-And-Mouth disease (HFMD) and some of EV71 infections may proceed to neurological diseases and even deat...
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| Format: | Monograph |
| Language: | English |
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Pusat Pengajian Sains Kesihatan, Universiti Sains Malaysia
2008
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| Online Access: | http://eprints.usm.my/49499/ http://eprints.usm.my/49499/1/GOH%20KAI%20WEY-24%20pages.pdf |
| _version_ | 1848881455466283008 |
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| author | Wey, Goh Kai |
| author_facet | Wey, Goh Kai |
| author_sort | Wey, Goh Kai |
| building | USM Institutional Repository |
| collection | Online Access |
| description | Enterovirus 71 (EV71) is a positive-sense single-stranded RNA, nonenveloped,
icosahedrally symmetrical virus which was first detected in 1969 in United States.
EV71 is associated with Hand-Foot-And-Mouth disease (HFMD) and some of EV71
infections may proceed to neurological diseases and even death because of neurogenic
pulmonary edema. To date, there is still no vaccine available against this disease.
Mycobacterium bovis Bacille Calmette-Guerin (BCG) has been suggested to be an
attractive vehicle for the delivery of foreign antigens to the immune system. The main
purpose of this study is to express a recombinant BCG (rBCG) containing ubiquitin
(UbGR)-VP1 fusion gene ofEV71. UbGR-VP1 protein was detected in the cell extract
but not in the supernatant of the recombinant clone by Western blot analysis using rabbit
polyclonal antibody specific to VP 1 protein. The immunogenicity of the rBCG is still
unknown and can only be confirmed if further study is done. |
| first_indexed | 2025-11-15T18:19:18Z |
| format | Monograph |
| id | usm-49499 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T18:19:18Z |
| publishDate | 2008 |
| publisher | Pusat Pengajian Sains Kesihatan, Universiti Sains Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-494992021-07-27T04:49:35Z http://eprints.usm.my/49499/ The expression of recombinant bacille calmette-guerin (rBCG) containing VPI gene of enterovirus 71 (EV71) Wey, Goh Kai R Medicine Enterovirus 71 (EV71) is a positive-sense single-stranded RNA, nonenveloped, icosahedrally symmetrical virus which was first detected in 1969 in United States. EV71 is associated with Hand-Foot-And-Mouth disease (HFMD) and some of EV71 infections may proceed to neurological diseases and even death because of neurogenic pulmonary edema. To date, there is still no vaccine available against this disease. Mycobacterium bovis Bacille Calmette-Guerin (BCG) has been suggested to be an attractive vehicle for the delivery of foreign antigens to the immune system. The main purpose of this study is to express a recombinant BCG (rBCG) containing ubiquitin (UbGR)-VP1 fusion gene ofEV71. UbGR-VP1 protein was detected in the cell extract but not in the supernatant of the recombinant clone by Western blot analysis using rabbit polyclonal antibody specific to VP 1 protein. The immunogenicity of the rBCG is still unknown and can only be confirmed if further study is done. Pusat Pengajian Sains Kesihatan, Universiti Sains Malaysia 2008 Monograph NonPeerReviewed application/pdf en http://eprints.usm.my/49499/1/GOH%20KAI%20WEY-24%20pages.pdf Wey, Goh Kai (2008) The expression of recombinant bacille calmette-guerin (rBCG) containing VPI gene of enterovirus 71 (EV71). Other. Pusat Pengajian Sains Kesihatan, Universiti Sains Malaysia. (Submitted) |
| spellingShingle | R Medicine Wey, Goh Kai The expression of recombinant bacille calmette-guerin (rBCG) containing VPI gene of enterovirus 71 (EV71) |
| title | The expression of recombinant
bacille calmette-guerin (rBCG) containing
VPI gene of enterovirus 71 (EV71) |
| title_full | The expression of recombinant
bacille calmette-guerin (rBCG) containing
VPI gene of enterovirus 71 (EV71) |
| title_fullStr | The expression of recombinant
bacille calmette-guerin (rBCG) containing
VPI gene of enterovirus 71 (EV71) |
| title_full_unstemmed | The expression of recombinant
bacille calmette-guerin (rBCG) containing
VPI gene of enterovirus 71 (EV71) |
| title_short | The expression of recombinant
bacille calmette-guerin (rBCG) containing
VPI gene of enterovirus 71 (EV71) |
| title_sort | expression of recombinant
bacille calmette-guerin (rbcg) containing
vpi gene of enterovirus 71 (ev71) |
| topic | R Medicine |
| url | http://eprints.usm.my/49499/ http://eprints.usm.my/49499/1/GOH%20KAI%20WEY-24%20pages.pdf |